Abstract: Since the early eighties, there has been a growing interest in the descriptive epidemiology of obsessive-compulsive disorder (OCD). In this narrative review, the authors describe the findings of a number of studies that employed selected instruments, such as the Diagnostic Interview Schedule, the Composite International Diagnostic Instrument, and the Schedule for Affective Disorders and Schizophrenia, to ascertain the prevalence and incidence rates for OCD in several different countries. We noted that there is a great heterogeneity of findings and that the potential reasons for this variability include not only the intrinsic characteristics of the population under study but also extrinsic factors (i.e., the several methodologically-informed decisions that are to be made before undertaking such investigations, such as the adoption of a specific diagnostic instrument). In order to further the knowledge on the epidemiology of OCD, it would be worthwhile to establish a global consensus regarding a standard assessment package for OCD, to produce more cross-culturally valid versions of the key research instruments, and to conduct studies specifically aimed at comparing the sociodemographic, clinical and prognostic aspects of OCD across different countries.

Abstract: Ninety-two surface sediment samples were collected in Guanabara Bay, one of the most prominent urban bays in SE Brazil, to investigate the spatial distribution of anthropogenic pollutants. The concentrations of heavy metals, organic carbon and particle size were examined in all samples. Large spatial variations of heavy metals and particle size were observed. The highest concentrations of heavy metals were found in the muddy sediments from the north western region of the bay near the main outlets of the most polluted rivers, municipal waste drainage systems and one of the major oil refineries. Another anomalous concentration of metals was found adjacent to Rio de Janeiro Harbour. The heavy metal concentrations decrease to the northeast, due to intact rivers and the mangrove systems in this area, and to the south where the sand fraction and open-marine processes dominate. The geochemical normalization of metal data to Li or Al has also demonstrated that the anthropogenic input of heavy metals have altered the natural sediment heavy metal distribution.

Abstract: The delayed x-ray detection technique was used to measure complete and incomplete fusion cross sections for the $^{9}\mathrm{Be}+^{144}\mathrm{Sm}$ reaction at sub- and near-barrier energies. Elastic and inelastic scattering for this system were also measured. Reaction cross sections were derived and the transfer cross sections of one neutron were calculated. The suppression of complete fusion above the barrier, of the order of 10%, is attributed to $^{9}\mathrm{Be}$ breakup and is considerably smaller than the value of 30% found for the $^{9}\mathrm{Be}+^{208}\mathrm{Pb}$ system.

Abstract: It is pointed out that the usual threshold anomaly, found operative in the energy behavior of the imaginary and real parts of the optical potential representing the elastic scattering of tightly bound nuclei at near- and below-barrier energies, suffers a drastic qualitative change in the case of the elastic scattering of weakly bound nuclei. Owing to the strong coupling to the breakup channel even at sub-barrier energies, the imaginary potential strength seems to increase as the energy is lowered to below the natural barrier threshold; this is accompanied by a decrease in the real potential strength. This feature is consistent with the dispersion relation. The system $^{6}\mathrm{Li}$+$^{208}\mathrm{Pb}$ is analyzed to illustrate this new phenomenon.

Abstract: We discuss shape (Pomeranchuk) instabilities of the Fermi surface of a two-dimensional Fermi system using bosonization. We consider in detail the quantum critical behavior of the transition of a two-dimensional Fermi fluid to a nematic state which breaks spontaneously the rotational invariance of the Fermi liquid. We show that higher dimensional bosonization reproduces the quantum critical behavior expected from the Hertz-Millis analysis, and verify that this theory has dynamic critical exponent $z=3$. Going beyond this framework, we study the behavior of the fermion degrees of freedom directly, and show that at quantum criticality as well as in the quantum nematic phase (except along a set of measure zero of symmetry-dictated directions) the quasiparticles of the normal Fermi liquid are generally wiped out. Instead, they exhibit short-ranged spatial correlations that decay faster than any power law, with the law $\ensuremath{\mid}x{\ensuremath{\mid}}^{\ensuremath{-}1}\mathrm{exp}(\ensuremath{-}\mathrm{const}\phantom{\rule{0.3em}{0ex}}\ensuremath{\mid}x{\ensuremath{\mid}}^{1∕3})$ and we verify explicitly the vanishing of the fermion residue utilizing this expression. In contrast, the fermion autocorrelation function has the behavior $\ensuremath{\mid}t{\ensuremath{\mid}}^{\ensuremath{-}1}\mathrm{exp}(\ensuremath{-}\mathrm{const}\phantom{\rule{0.3em}{0ex}}\ensuremath{\mid}t{\ensuremath{\mid}}^{\ensuremath{-}2∕3})$. In this regime we also find that, at low frequency, the single-particle fermion density of states behaves as ${N}^{*}(\ensuremath{\omega})={N}^{*}(0)+B{\ensuremath{\omega}}^{2∕3}\mathrm{ln}\ensuremath{\omega}+\ensuremath{\cdots}$, where ${N}^{*}(0)$ is larger than the free Fermi value, $N(0)$, and $B$ is a constant. These results confirm the non-Fermi liquid nature of both the quantum critical theory and of the nematic phase.

Abstract: Community structure and diet of roving herbivorous reef fishes were analysed in 13 study sites around the five islands of the Abrolhos Archipelago, north-eastern Brazil (17°58' S; 38°42' W). This area has been part of the Abrolhos Marine National Park since 1983. Abundances and diets of fishes within the families Scaridae, Acanthuridae and Kyphosidae were compared among groups of sites differing in benthic community structure and exposure regime. The abundance of roving herbivorous fishes was higher in shallower sites than in deeper sites. At all study sites, observations of total herbivorous reef fish community structure revealed that 64% of fishes were acanthurids, 33% were scarids and only 4% were kyphosids. This pattern was predominant in all study sites. The majority of fishes examined in this study had the bulk of their diet based on both algae (35-90%) and detritus (35-65%). Among groups of algae, filamentous algae were the most commonly consumed as the majority of roving herbivorous fishes in Abrolhos feed as scrapers and excavators. The exceptions were the kyphosids, which had a diet consisting primarily of macroalgae (mainly Phaeophyta), and Acanthurus coeruleus that consumed mainly turf algae and very little detritus. The data collected indicate that roving herbivorous fishes in the Abrolhos Archipelago are more abundant at calm sites where detritus and delicate algae, their major food resources, are more abundant. The results presented here, along with underwater observations, suggest that the abundance and diet of these three fish families are associated with substratum cover.

Abstract: Methicillin-resistant Staphylococcus aureus (MRSA) and coagulase-negative staphylococcus infections are a worldwide concern. Currently, these isolates have also shown resistance to vancomycin, the last therapy used in these cases. It has been observed that quinones and other related compounds exhibit antibacterial activity. This study evaluated the antibacterial activity, toxicity and in vivo dermal irritability of lapachol extracted from Tabebuia avellanedae and derivatives against methicillin-resistant staphylococcal isolates. In addition, its mechanism of action was also analyzed. The compounds β-lapachone, 3-hydroxy β N lapachone and α-lapachone were tested to determine the MIC values against methicillin-resistant S. aureus, S. epidermidis and S. haemolyticus strains, being the two last ones hetero-resistant to vancomycin. Experiments of protein synthesis analysis to investigate the naphthoquinones action were assessed. In vitro toxicity to eukaryotic BSC-40 African Green Monkey Kidney cell cultures and in vivo primary dermal irritability in healthy rabbits were also performed. The compounds tested showed antibacterial activity (MICs of 8, 4/8 and 64/128 μg/mL to β-lapachone, 3-hydroxy β N lapachone and α-lapachone, respectively), but no bactericidal activity was observed (MBC > 512 μg/mL for all compounds). Although it has been observed toxic effect in eukaryotic cells, the compounds were shown to be atoxic when applied as topic preparations in healthy rabbits. No inhibition of proteins synthesis was observed. Our results suggest that quinones could be used in topic preparations against wound infections caused by staphylococci, after major investigation of the pharmacological properties of the compounds. Studies about the use of these compounds on tumoral cells could be carried on, due to their effect in eukaryotic cells metabolism.

Abstract: The mechanism by which the virus associated with dengue fever can cause a fatal hepatitis is not well understood. The purpose of this study was to examine 9 cases of fatal dengue hemorrhagic fever-associated hepatitis, and to correlate the histologic findings with viral detection and cytokine response. The histologic changes were nonspecific and included massive hepatic necrosis and a pauci-cellular acute hepatitis. Viral cDNA detection by reverse transcriptase in situ polymerase chain reaction demonstrated that the fatal hepatitis was due to infection on average of >90% of hepatocytes and many Kupffer cells. Similar results were obtained using immunohistochemistry for viral protein using an automated highly sensitive system. Immunohistochemical analysis for tumor necrosis factor alpha, and interleukin-2, showed rare positive Kupffer cells. In comparison, fatal cases of hepatitis C associated liver failure demonstrated far fewer infected hepatocytes and a concomitant strong up-regulation of many cytokines, notably tumor necrosis factor alpha and interleukin-2. It is concluded that fatal dengue hemorrhagic fever is associated with acute, severe liver damage due primarily to massive direct infection of hepatocytes and Kupffer cells with minimal cytokine response. The infection can be readily detected in a few hours using an automated system that has a sensitivity equivalent to reverse transcriptase in situ polymerase chain reaction.

Abstract: de Carvalho Maciel AC, Zaccaro Scelza MF. Efficacy of automated versus hand instrumentation during root canal retreatment: an ex vivo study. International Endodontic Journal, 39, 779‐784, 2006. Aim To compare automated and manual instrumentation techniques for removing filling material from root canal walls during root canal retreatment. Methodology One hundred extracted human single-rooted teeth were root filled and stored. Specimens were divided into two groups: group A, Endofill plus gutta-percha; group B, Sealer 26 plus gutta-percha. The filling material was removed using the following techniques: group I – Gates–Glidden and K-type files; group II – ProFile; group III – ProTaper; group IV – K3; group V– Micro Mega Hero 642. The remaining filling debris on the root canal walls were assessed radiographically, images were digitized and analysed using Image ProPlus software. The roots were split for evaluation in a stereomicroscope by epiluminescence and photomicrographs were taken for further analysis. The area covered with filling debris was analysed by means of Student’s t-test to compare the evaluation methods. The student’s t-test was also used to compare the removal of filling materials. An anova test was applied to compare the different techniques (P 0.05). Manual instrumentation left more filling debris on the root canal walls when compared to K3 (P < 0.05) and ProTaper (P < 0.01). Conclusions A photomicrographic method by epiluminescence was more effective than the radiographic method to evaluate filling debris. There was no significant difference between the filling materials in terms of their removal. K3 and ProTaper were more efficient than manual instrumentation.

Abstract: TMAs are becoming a useful tool for research and quality control methods, mostly for immunohistochemistry and in situ hybridization. A new technique that allows building TMA blocks with more than 300 tissue cores without using a recipient paraffin block for the tissue cores and without using a commercial TMA builder instrument is described. This technique is based on the construction of TMA needles modifying conventional hypodermic needles to punch tissue cores from donor blocks, which are attached by double-side adhesive tape on a computer-generated paper grid used to align the cores on the block mould, which is filled with liquid paraffin. More than two hundred TMA blocks were constructed using this method, utilized in immunohistochemistry and histochemistry as positive and negative controls and also in research. This technique has the following advantages: it is easy to reproduce, affordable, quick and creates uniform blocks with more than 300 cores aligned, adherent and easy to cut, with negligible losses during cutting and immunohistochemistry and in situ hybridization procedures.

Abstract: This paper presents the results of a 22-month survey and the examination of the intestinal content of 356 specimens of Xenohyla truncata (Anura: Hylidae) from Restinga de Maric ´ a, Rio de Janeiro, Brazil Our results confirm prior observations that fruits are intrinsic to the diet of these frogs In addition, these new data increase our understanding of the relationship between frogs and the plants they feed upon Plant consumption follows availability of fruits in the area, indicating that the diversity of fruits consumed by the frogs does not represent choice, but rather plant phenology and fruit availability