Zuncai Wang
Novartis
8 Papers
124 Citations
Zuncai Wang is an academic researcher from Novartis. The author has contributed to research in topics: Autophagy & CRISPR. The author has an hindex of 7, co-authored 8 publications.
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Papers
Selective VPS34 inhibitor blocks autophagy and uncovers a role for NCOA4 in ferritin degradation and iron homeostasis in vivo
William E. Dowdle,Beat Nyfeler,Jane Nagel,Robert Elling,Shanming Liu,Ellen Triantafellow,Suchithra Menon,Zuncai Wang,Ayako Honda,Gwynn Pardee,John Cantwell,Catherine Luu,Ivan Cornella-Taracido,Edmund Harrington,Peter Fekkes,Hong Lei,Qing Fang,Mary Ellen Digan,Debra Burdick,Andrew F. Powers,Stephen B. Helliwell,Simon D’Aquin,Julie Bastien,Henry Wang,Dmitri Wiederschain,Jenny Kuerth,Philip Bergman,David Schwalb,Jason R. Thomas,Savuth Ugwonali,Fred Harbinski,John A. Tallarico,Christine D. Wilson,Vic E. Myer,Jeffery A. Porter,Dirksen E. Bussiere,Peter Finan,Mark Labow,Xiaohong Mao,Lawrence G. Hamann,Brendan D. Manning,Reginald Valdez,Thomas B. Nicholson,Markus Schirle,Mark Knapp,Erin P. Keaney,Leon Murphy +46 more
TL;DR: a4−/− mice exhibit a profound accumulation of iron in splenic macrophages, which are critical for the reutilization of iron from engulfed red blood cells, and a previously unappreciated role for autophagy and NCOA4 in the control of iron homeostasis in vivo is revealed.
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Macroautophagy is dispensable for growth of KRAS mutant tumors and chloroquine efficacy
Christina H. Eng,Zuncai Wang,Diane Tkach,Lourdes Toral-Barza,Savuth Ugwonali,Shanming Liu,Stephanie Fitzgerald,Elizabeth George,Elizabeth Frias,Nadire Cochran,Rowena De Jesus,Gregory McAllister,Gregory R. Hoffman,Kevin Bray,Luanna Lemon,Judy Lucas,Valeria Fantin,Robert T. Abraham,Leon Murphy,Beat Nyfeler +19 more
TL;DR: Data indicate that KRAS mutation status does not predict cell-autonomous addiction to autophagy, and addresses a long-standing question regarding the mechanism of chloroquine, a lysosomotropic agent often used as a pharmacological tool to evaluate the response to macroautophagy inhibition.
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Autophagy-Independent Lysosomal Targeting Regulated by ULK1/2-FIP200 and ATG9
Jonathan M. Goodwin,William E. Dowdle,Rowena DeJesus,Zuncai Wang,Philip Bergman,Marek J. Kobylarz,Alicia Lindeman,Ramnik J. Xavier,Gregory McAllister,Beat Nyfeler,Gregory R. Hoffman,Leon Murphy +11 more
TL;DR: Using genome-scale functional screening, this work identifies an alternative lysosomal transport pathway for ferritin that requires FIP200, ATG9A, VPS34, and TAX1BP1 but lacks involvement of the ATG8 lipidation machinery that constitutes classical macroautophagy.
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Functional CRISPR screening identifies the ufmylation pathway as a regulator of SQSTM1/p62
Rowena DeJesus,Francesca Moretti,Gregory McAllister,Zuncai Wang,Phil Bergman,Shanming Liu,Elizabeth Frias,John Alford,John S. Reece-Hoyes,Alicia Lindeman,Jennifer Kelliher,Carsten Russ,Judith Knehr,Walter Carbone,Martin Beibel,Guglielmo Roma,Aylwin Ng,John A. Tallarico,Jeffery A. Porter,Ramnik J. Xavier,Craig Mickanin,Leon Murphy,Gregory R. Hoffman,Beat Nyfeler +23 more
TL;DR: In this article, a forward genetic screening paradigm exploiting CRISPR-mediated genome editing coupled with a cell selection step by FACS was described to identify regulators of SQSTM1.
Functional CRISPR screening identifies the ufmylation pathway as a regulator of
Rowena DeJesus,Francesca Moretti,Gregory McAllister,Zuncai Wang,Phil Bergman,Shanming Liu,Elizabeth Frias,John Alford,John S. Reece-Hoyes,Alicia Lindeman,Jennifer Kelliher,Carsten Russ,Judith Knehr,Walter Carbone,Martin Beibel,Guglielmo Roma,Aylwin Ng,John A. Tallarico,Jeffery A. Porter,Ramnik J. Xavier,Craig Mickanin,Leon Murphy,Gregory R. Hoffman,Beat Nyfeler +23 more
- 01 Jan 2016
TL;DR: It is shown that ufmylation regulates SQSTM1 by eliciting a cell type-specific ER stress response which induces SQ STM1 expression and results in its accumulation in the cytosol, validating pooled CRISPR screening as a powerful method to map the repertoire of cellular pathways that regulate the fate of an individual target protein.
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