The invention relates to the fields of genetic engineering and molecular biology, and discloses an AGTR1 gene detection kit. The detection kit comprises a primer group for specifically amplifying a nucleic acid fragment containing an rs5186 site, the primer group comprises an upstream primer represented by SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3 or SEQ ID NO:4, and a downstream primer represented bySEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7 or SEQ ID NO:8. The invention also provides a gene detection method. The kit uses the specific amplification primer group to amplify the nucleic acid fragment containing the rs5186 site, so the detection sensitivity of AGTR1 gene mutation is high, and the false positive rate is low; and the kit adopts a second generation high-throughput gene sequencing technology to detect, so short detection period and high throughput are achieved.

AGTR1 gene detection kit and AGTR1 gene detection method

The invention provides an essential hypertension gene noninvasive detection kit, which includes: specific primers and DNA sequencing primers for detecting the genotypes of 6 single nucleotide polymorphic sites, ie I/D on ACE gene, A1166C on AGTR1 gene, G894T on NOS3 gene, M235T on AGT gene, Arg16Gly on ADRB2 gene and C-344T on CYP11B2 gene, a PCR reaction component, a PCR product purification component, and a DNA sequencing reaction component, etc By detecting the genotypes of 6 single nucleotide polymorphic sites closely related to the occurrence of essential hypertension, the kit provided in the invention evaluates the risk level of essential hypertension attack to people at the gene level, thus guiding people to prevent the disease in advance The sampling method adopted by the invention is oral mucosa cell sampling, is painless, noninvasive, and avoids cross infection The sequencing results are accurate and reliable, there exists no need to purchase expensive imported special equipment, and the method is easy for popularization and promotion

Essential hypertension gene noninvasive detection kit

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Essential hypertension gene noninvasive detection kit