Zhao Hongcui
3 Papers
3 Citations
Zhao Hongcui is an academic researcher. The author has contributed to research in topics: Homologous recombination & Gene. The author has an hindex of 2, co-authored 3 publications.
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Papers
Deep functional analysis of synII, a 770-kilobase synthetic yeast chromosome
Yue Shen,Yun Wang,Tai Chen,Feng Gao,Jianhui Gong,Dariusz Abramczyk,Roy Walker,Zhao Hongcui,Shihong Chen,Wei Liu,Yisha Luo,Carolin A. Müller,Adrien Paul-Dubois-Taine,Bonnie Alver,Giovanni Stracquadanio,Giovanni Stracquadanio,Leslie A. Mitchell,Zhouqing Luo,Yanqun Fan,Baojin Zhou,Bo Wen,Fengji Tan,Yujia Wang,Jin Zi,Ze-Xiong Xie,Bing-Zhi Li,Kun Yang,Sarah M. Richardson,Hui Jiang,Christopher E. French,Conrad A. Nieduszynski,Romain Koszul,Adele L. Marston,Ying-Jin Yuan,Jian Wang,Joel S. Bader,Joel S. Bader,Junbiao Dai,Jef D. Boeke,Xun Xu,Yizhi Cai,Huanming Yang +41 more
TL;DR: It is demonstrated that synII segregates, replicates, and functions in a highly similar fashion compared with its wild-type counterpart, and the iterative “design-build-test-debug” cycle methodology will facilitate progression of the Sc2.0 project in the face of the increasing synthetic genome complexity.
Patent
Saccharomyces cerevisiae strain with artificially synthesized chromosome II
Chen Tai,Gao Feng,Wang Yun,Yue Shen,Zhao Hongcui,Tian Geng,Xu Xun +6 more
- 03 Aug 2016
TL;DR: Wang et al. as discussed by the authors provided a saccharomyces cerevisiae strain for artificial synthesis and a purpose thereof, which was used for optimizing production of a metabolite; a mutant database is generated by inducing SCRaMbLE, and is used for screening bacterial strains having various characteristics.
3
Patent
Method and kit for DNA fragment assembling in vitro
Chen Tai,Zhao Hongcui,Fan Chuyao,Chen Shihong,Wang Yun,Shen Yue,Xu Xun +6 more
- 27 Apr 2017
Abstract: Disclosed in the present invention are a method and a kit for DNA fragment assembling in vitro, being able to assemble a plurality of DNA fragments with an assembling vector in a set fragment order. The upstream of the first fragment and the downstream of the last fragment respectively carry an overlapping sequence being same as the assembling vector. With respect to the other fragments, the upstream of each fragment carries an overlapping sequence being same as the downstream of the previous fragment, and the downstream of each fragment carries an overlapping sequence being same as the upstream of the next fragment. The inner side of the overlapping sequence of the assembling vector optionally carries restriction enzyme cutting sites respectively, and optionally carries selectable markers between the restriction enzyme cutting sites. The method of the present invention enables a plurality of DNA fragments and the assembling vector to react with DNA endonuclease, DNA exonuclease, DNA polymerase and DNA ligase, so as to accomplish the assembly.