Zekun Guo

TL;DR: It is shown in bovine fetal fibroblasts that targeting ZFNickases to the endogenous β-casein (CSN2) locus stimulates lysostaphin gene addition by homology-directed repair, and it is found that ZF Nickase-treated cells can be successfully used in somatic cell nuclear transfer, resulting in live-born gene-targeted cows.

TL;DR: It is found that BCB+ oocytes have a higher nuclear reprogramming capacity, and thatBCB staining can be used to select developmentally competent oocytes for nuclear transfer.

TL;DR: It is demonstrated that retinoid induces mESCs to differentiate by regulating miR-200b/200c, which provides the landscapes of mRNA and microRNA gene networks and indicate the crucial role ofmiR- 200b/ 200c in the RA-induced differentiation of m ESCs.

TL;DR: It is strongly suggested that vitamin C promoted widespread DNA demethylation in gene promoters by modulating epigenetic modifiers, including Dnmt3a, which activated pluripotency genes and ESC‐specific miRNAs.