With high fecundity and short production cycle, poultry is one of the important sources of meat. During the embryonic and post-hatch period, the higher death rate caused huge economic losses in poultry production. Our previous study showed that chick subcutaneous adipose tissue is an important energy supply tissue besides yolk. Therefore, the metabolic mechanism of subcutaneous adipose tissue in chicks could provide a new perspective of brooding. The objectives of the current study were to evaluate the differences between chick subcutaneous adipose tissue and abdominal adipose tissue before and after hatching and reveal the cross-talk of different cells within the chick subcutaneous adipose tissue. The results of RNA-seq and weighted gene co-expression network analysis (WGCNA) of chick subcutaneous and abdominal adipose tissues showed that the function of chick subcutaneous tissue was related to immunoreaction, and macrophage could be the major immune infiltration cell type in chicken subcutaneous adipose tissue, which were also verified by qPCR, HE stain, and IHC. The results of free fatty acids (FFAs)-induced the cross-talk between macrophages and adipocytes showed that FFAs-Ccl2 (chicken CCL26) axis could have an important role in lipid transportation in adipose tissue. The results of Oil Red O and Nile red stain demonstrated that macrophages have the ability to absorb FFAs quickly. Interestingly, according to the genomic organization of CCL family with representative vertebrate species, we found that chicken CCL26 could be the major chemokine in chicken adipocyte as the status of CCL2 in mammal adipocyte. In conclusion, we demonstrate that FFA-induced Ccl2 (chicken CCL26) secretion is crucial in determining fat depot-selective adipose tissue macrophage (ATM) infiltration, which could be an important medium of lipid transportation in chicken subcutaneous adipose tissue. These findings may have multiple important implications for understanding macrophage biology with chick subcutaneous adipose tissue and provide theoretical basis for lipid metabolism in poultry brooding.

/pdf/rna-seq-based-transcriptome-analysis-reveals-the-cross-talk-1pi25zmz.pdf

RNA-seq Based Transcriptome Analysis Reveals The Cross-Talk of Macrophage and Adipocyte of Chicken Subcutaneous Adipose Tissue during The Embryonic and Post-Hatch Period

Over the last few decades, progress in molecular biology and genetic technologies has allowed scientists to explore the mechanisms of lipid metabolism in chickens. Through techniques like RNA sequencing and gene expression profiling, researchers can now investigate how dietary fat supplements impact the expression of particular genes related to lipid metabolism regulation. Limited research exists on the comparative impacts of fat powder and fatty acid incorporation in broiler feed on the expression of lipid metabolism-regulating genes in the liver. This study aims to identify particular genes and metabolic pathways linked to reduced abdominal fat deposition in commercial broiler chickens. The study involved 500 broiler chickens from the commercial strain Ross 308, distributed in a completely randomized layout with five different treatments and five replications. The duration of the research lasted for 42 days. Initially, the control group received no fatty acid or fat powder, whereas the other groups were supplemented with either 3% or 6% fat powder or fatty acid. The experimental period was divided into two distinct phases: the starter phase (0-21 days) and the grower phase (22-41 days). Fatty acid synthase (FASN), malic enzyme 1 (ME1), stearoyl-CoA desaturase (SCD), glycerol-3-phosphate acyltransferase 3 (GPAT3), glycerol-3-phosphate acyltransferase, mitochondrial (GPAM), CD36 molecule (CD36), carnitine palmitoyltransferase 1A (CPT1A), carnitine palmitoyltransferase 2 (CPT2), acyl-CoA oxidase 1 (ACOX1), apolipoprotein B (APOB), very low-density apolipoprotein II (apoVLDLII), peroxisome proliferator-activated receptor alpha (PPARα), peroxisome proliferator-activated receptor gamma (PPARγ) genes selected as indicators of lipid metabolism-regulation and were studied using qPCR technique. The impact of fatty acids (FA) on the regulation of genes under investigation was found to be more pronounced. However, both types of fat increased abdominal fat, with FA demonstrating a stronger effect. While the fat level was deemed significant in certain instances, the current findings and methodology suggest that a 3% fat supplement may be a more cost-effective and economical option for farm use. To further enhance the initial findings and gain a more comprehensive understanding of fat metabolism in poultry, future studies should involve comparative and correlative analyses, encompass a wider range of fat sources, and consider production traits in conjunction with genes associated with fatty acids in the liver. 

pdf/investigating-the-impact-of-various-fat-supplements-and-3gz0245uwy.pdf

Investigating the impact of various fat supplements and their levels on the expression of lipid metabolism-regulated genes in the liver tissue of broiler chickens

Abstract The avian skin is a vital barrier against external effects and undergoes modification to adapt to the different ecosystems. The current study focused on the comprehensive study of the scaly shank skin of aquatic birds, Egyptian Balady Duck (EBD) as well as terrestrial birds, Broad Breasted White Turkey (BBWT) via gross anatomy, histology, and scanning electron microscopy with ED-XRF analysis. The shank skin color was yellow to black in EBD and creamy-white in BBWT. Gross anatomy exhibited two types of scales around the shank: scute and scutella scales in EBD and four types: scute, scutella, reticula, and cancella (interstitial) scales in BBWT. Most scales were non-overlapped and separated by sulci in both birds except those on the dorsum of the shank of BBWT. SEM of the descaled skin revealed an irregular surface due to keratinocytes defining a polygonal texture in EBD and Langerhans cells (a large oval-shaped cell body with abundant long projections) attached to neighboring keratinocytes in BBWT. Histologically, the epidermal and dermal layers varied among the different skin aspects of the shanks of both birds. Langerhans cells were seen within the basal cell layer of the stratum germinativum and collagen fibers of the dermal stratum superficiale. Melanocytes were observed in the stratum basale in EBD. In both birds, abundant telocytes and fibroblasts were distributed within the dermal layers, with excessive adipose tissue in the dermis of the shank skin of EBD. According to the results of the ED-XRF analysis of the scaly shank skin, elements and oxides were present in both species. In conclusion, the findings of the present study reflect the correlations between the functional morphology of shank skin and the bird habitats. 

Unique insights into morphological characterization and functional adaptation of the scaly shank skin in aquatic and terrestrial birds

Since excess abdominal fat is one of the main problems in the broiler industry for the development of modern broiler and layer industry, the importance of subcutaneous adipose tissue has been neglected. However, chick subcutaneous adipose tissue appeared earlier than abdominal adipose tissue and more than abdominal adipose tissue. Despite a wealth of data, detailed information is lacking about the development and function of chick subcutaneous adipose tissue during the embryonic and posthatch period. Therefore, the objective of the current study was to determine the developmental changes of adipocyte differentiation, lipid synthesis, lipolysis, fatty acid β-oxidation, and lipid contents from E12 to D9.5. The results showed that subcutaneous adipose tissue was another important energy supply tissue during the posthatch period. In this stage, the mitochondrial copy number and fatty acid β-oxidation level significantly increased. It revealed that chick subcutaneous adipose tissue not only has the function of energy supply by lipidolysis but also performs the same function as brown adipose tissue to some extent, despite that the brown adipose tissue does not exist in birds. In addition, this finding improved the theory of energy supply in the embryonic and posthatch period and might provide theoretical basis on physiological characteristics of lipid metabolism in chicks.

/pdf/function-of-chick-subcutaneous-adipose-tissue-during-the-3qp7hpqwbr.pdf

Function of Chick Subcutaneous Adipose Tissue During the Embryonic and Posthatch Period

Handle everyday research tasks with reliable, citation-backed results

Your personal Research Agent to handle research tasks with citation-backed results

Popular Tasks used by Researchers

How can I help with your research?

Meet SciSpace

Get more enhanced response by uploading the PDFs you want me to reference.

No relevant PDFs in your library

SciSpace is the AI research assistant for academics. Run systematic literature reviews on 280M+ papers, and write papers with cited sources. Trusted by 1M+ students, PhDs & researchers.

SciSpace | AI for Research

Analyze PDFs

Code & Manuscripts

Funding & Grants

Literature & Patents

Medical & Clinical Data

Systematic Review

Visualize & Present

Web & Data

Build a Google Scholar-like website for your research.

Build a website

Create charts and images for your research

Create a Chart

Write a paper for submission to a journal

Draft a manuscript

Patent Search

Design eye-catching scientific posters in minutes.

Scientific Poster Generation

Systematic Literature Review

One task is running at the moment. Your messages will be shown right after.

Drag and drop or click here to browse

Loved by <highlight>1 million+</highlight> researchers

Extract a list of specific topics and their sources from unstructured text

Topics

Compare and analyze relevant papers that matches with your search

Papers

Get insights from PDFs and bookmarked papers from your library

My library

Recent searches

Try searching for:

Catch AI-generated content in scholarly and non-scholarly content

{ai} Detector

Ai Writer

Get PDF Summaries, highlighted text explanations 

Chat with PDF

Effortlessly create in-text citations and bibliographies in APA and 2,500 other formats

Citation generator

Get explanations, summaries, and answers on academic papers

Ease up your research workflow with {scispace}'s cohort of exciting AI tools

Elevate your academic writing skills and convey your ideas the way you want

Paraphraser

Explore our range of reading and writing tools

Your file is being prepared and should be ready in a few minutes. If it's a large file, it might take a bit longer. You can close this window, and we'll email you the file when it's done.

You have reached a maximum limit of <strong>{limit}</strong> columns in the table. Remove at least <strong>1</strong> column to add or create another one.

Zehui Wei

Author Tools

Chat about Author

Papers

Function of Chick Subcutaneous Adipose Tissue During the Embryonic and Posthatch Period