
 Youjun Yang (left) received his BS (2002) from the University of Science and Technology of China, and his PhD (2007) with Prof. Robert M. Strongin at Louisiana State University. Then, he joined the Anslyn group at UT Austin as a postdoc. In 2010, he joined the faculty of the school of pharmacy, East China University of Science and Technology (ECUST). His research interests include NIR fluorescent dyes, photo-responsive dyes, and fluorescent molecular probes.
 Jiangli Fan (right) obtained her PhD at Dalian University of Technology (2005). Now she is a Professor at the School of Chemical Engineering at Dalian University of Technology, and she received the Distinguished Young Scholars of NSFC (2019). Her research interests mainly focus on the development of functional dyes for biomedical analysis and disease treatment.

Cluster Preface: Functional Dyes

Rapidly capturing slight changes in cell surface pH is extremely important to evaluate the rapid diffusion of acidic metabolites into the extracellular environment caused by disease and physiological pH fluctuations of cells. In this work, we designed a membrane-targeted pH probe, Mem-C0C18, based on a novel heterocyclic xanthene-analogous backbone. Mem-C0C18 shows specific and stable staining ability towards membrane. Importantly, the fluorescence lifetime of Mem-C0C18 is highly sensitive against acidity within membrane, which is in favor of quantifying pH through fluorescence lifetime imaging. Using Mem-C0C18, we recorded pH changes of 0.61 units on the surface of human cervical cancer cells (Hela) during glycolysis. Further on, we observed a robust pH-regulating mechanism of the plasma membrane that the pH fluctuation range within membrane (5.32-6.85) is much smaller than the change in extracellular environment (4.00-8.00). Consequently, we demonstrate a pH probe for quantifying small pH fluctuations within cell membrane that merits further evaluation for biology applications.

A Novel Heterocyclic Xanthene-Analogous pH Probe for Quantitative Monitoring of Cell Surface pH by Fluorescence Lifetime Imaging

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A Novel Heterocyclic Xanthene-Analogous pH Probe for Quantitative Monitoring of Cell Surface pH by Fluorescence Lifetime Imaging