Zbyszek Otwinowski
University of Texas Southwestern Medical Center
127 Papers
575 Citations
Zbyszek Otwinowski is an academic researcher from University of Texas Southwestern Medical Center. The author has contributed to research in topics: Biology & Protein structure. The author has an hindex of 43, co-authored 115 publications. Previous affiliations of Zbyszek Otwinowski include University of Illinois at Chicago & University of Texas at Dallas.
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Papers
Structure of the effector-binding domain of the arabinose repressor AraR from Bacillus subtilis.
Kateřina Procházková,Kateřina Čermáková,Petr Pachl,Irena Sieglová,Milan Fábry,Zbyszek Otwinowski,Pavlína Řezáčová +6 more
TL;DR: The crystal structure of the C-terminal effector-binding domain of AraR in complex with the effector L-arabinose has been determined and detailed analysis of the crystal identified a dimer organization which is distinctive from that of other members of the GalR/LacI family.
Structural characterization of V57D and V57P mutants of human cystatin C, an amyloidogenic protein.
Marta Orlikowska,Aneta Szymańska,Dominika Borek,Zbyszek Otwinowski,Piotr M. Skowron,Elżbieta Jankowska +5 more
TL;DR: Additional hCC mutants were obtained in order to elucidate the relationship between the stability of the L1 loop and the propensity of human cystatin C to dimerize and their dimeric architecture is discussed in terms of the stabilization and destabilization effects of the introduced mutations.
The crystal structure of the effector-binding domain of the trehalose repressor TreR from Bacillus subtilis 168 reveals a unique quarternary assembly†
Pavlína Řezáčová,Veronika Krejčiříková,Veronika Krejčiříková,Dominika Borek,S. Moy,Andrzej Joachimiak,Zbyszek Otwinowski +6 more
TL;DR: The crystal structure of the TreR effector-binding domain is presented using diffraction data to reveal a unique oligomeric assembly that is likely important for TreR function.
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Crystal structure of native β-N-acetylhexosaminidase isolated from Aspergillus oryzae sheds light onto its substrate specificity, high stability, and regulation by propeptide.
Jana Škerlová,Jan Bláha,Petr Pachl,Kateřina Hofbauerová,Kateřina Hofbauerová,Zdeněk Kukačka,Zdeněk Kukačka,Petr Man,Petr Man,Petr Pompach,Petr Novák,Petr Novák,Zbyszek Otwinowski,Jiří Brynda,Ondřej Vaněk,Pavlína Řezáčová +15 more
TL;DR: The crystal structure reported here provides structural insights into the enzyme architecture as well as the detailed configuration of the active site that can be applied to rational enzyme engineering.
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Crystallization and preliminary X-ray analysis of Ebola VP35 interferon inhibitory domain mutant proteins
Daisy W. Leung,Dominika Borek,Mina Farahbakhsh,Parameshwaran Ramanan,Jay C. Nix,Tianjiao Wang,Kathleen C. Prins,Zbyszek Otwinowski,Richard B. Honzatko,Luke A. Helgeson,Christopher F. Basler,Gaya K. Amarasinghe +11 more
TL;DR: The expression, purification and crystallization of recombinant Zaire Ebola VP35 IID mutants R312A, K319A/R322A and K339A in space groups P6(1)22, P2 (1)2(1).2 and P2(2), respectively, are described.
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