We determined cytosol c-erbB-2 protein levels using a sandwich enzyme immunoassay in benign breast disease and primary and recurrent breast cancer and analyzed the relationship between c-erbB-2 protein levels and clinicopathological factors. Overexpression of c-erbB-2 protein, the cut-off value being set at 18 ng/mg protein, was observed in 26 of the 139 cases of stages I-IIIB breast cancer (18.7%), four of the 12 cases of stage IV breast cancer (33.3%) and seven of the 13 recurrent breast cancer cases (53.8%). The levels of c-erbB-2 protein were significantly different between the stages. Overexpression of c-erbB-2 protein in stages I-IIIB breast cancer was associated with histological grade and serum CEA level, but not with other clinicopathological factors. In addition, there was an inverse correlation in the group of stages I-III plus IV breast cancer between c-erbB-2 protein expression and estrogen receptor status. Overexpression of c-erbB-2 protein can be easily determined in the cytosol fraction together with hormonal receptor by this method. The prognostic importance will be evaluated in ongoing adjuvant trials for operable breast cancer patients.

Determination of Cytosol c-erbB-2 Protein in Breast Cancer by Sandwich Enzyme Immunoassay

ErbB-2 protein levels in breast cancer extracts in 128 patients were mesured by EIA, and compared with other well-known prognostic factors. In primary breast cancer, 12.3% (15/122) of the cases were found to be positive for ErbB-2 protein, and 33.3% (2/6) of the non-primary cases were positive for ErbB-2 protein (the cut-off value of ErbB-2 protein was set at 18.0ng/mg prot) Overexpression of ErbB-2 protein was significantly associated with lymphatic vessel invasion (p=0.0158) and negative estrogen receptor (p=0.0127). It also related to large tumor size, but there was no significant difference. However, overespression of ErbB-2 was not associated with age and lymph node metastases. These results suggest that the commbined use of classical prognostic factors and mesurement of ErbB-2 protein may improve prognostic value and contribute to adjuvant chemotherapy and hormonal therapy.

https://www.jstage.jst.go.jp/article/ringe1963/58/1/58_1_8/_pdf

EVALUATIN OF ErbB-2 PROTEIN IN TISSUE EXTRACT COMPARED WITH OTHER PROGNOSTIC FACTORS IN HUMAN BREAST CANCERS

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EVALUATIN OF ErbB-2 PROTEIN IN TISSUE EXTRACT COMPARED WITH OTHER PROGNOSTIC FACTORS IN HUMAN BREAST CANCERS