Abstract Background The reliance on chemical inputs to support high yields is the Achilles’ heel of modern crop production. The soil organic matter management is as old as agriculture itself. Recently, the use of soluble humic substances as plant growth promoters has been brought to attention due to their effects on nutrient uptake and water use efficiency. Humic substances applied directly at low concentrations can trigger different molecular, biochemical, and physiological processes in plants. However, how humic substances exert this plethoric regulatory action remains unclear. The objective of this study was to evaluate changes in the transcription level of genes coding cell receptors, phosphatases, synthesis, and function of different plant hormones and transcription factors. Materials and methods After seven days of humic acid treatment, we used RNAseq in maize root seedlings. The level of gene transcription was compared with control plants. Results Plant kinase receptors and different phosphatases were regulated by humic acids. Likewise, genes related to plant hormones (auxin, gibberellin, ethylene, cytokinin, abscisic acid, brassinosteroids, jasmonic and salicylic acids) were transcript in differential levels in maize root seedlings as well as the expression of a hundred of transcription factors modifying the signal transduction pathway via alterations of the subsequent gene response. Conclusion We showed a general mechanism for simultaneously regulating the activity of several hormones where humic acids act as a key regulatory hub in plant responses integrating hormonal signalling and response pathways. 

/pdf/plant-hormone-crosstalk-mediated-by-humic-acids-3qtmf8e6.pdf

Plant hormone crosstalk mediated by humic acids

/pdf/plant-hormone-crosstalk-mediated-by-humic-acids-3dcfefz0.pdf

Worldwide Research Trends on Microalgae and Recent Work in Cytologia

Next Generation Sequence-based Technologies for Analyzing DNA Strand Breaks

Regeneration is the process of reconstituting or developing new cells, tissues, and organs. Recent research has revealed that the priming mechanism plays a crucial role in regeneration. This mechanism involves the cell entering a primed state after the first stimulus, which could be a change in differentiation state, metabolic alteration, inflammatory response due to pathogen stimulation, or environmental stimuli. In this primed state after the initial stimulus, histone modifications and chromatin open/closed states remain in the primed gene loci. Upon arrival of the second stimulus, whether the same or different, these epigenetically primed genes are transcribed more quickly and potently than in response to the first stimulus. This is because RNA polymerase II can bind to the promoters of the primed genes immediately. This mechanism of priming enables quick shoot regeneration from callus in plants and prompt wound repair in mammals. This review focuses on recent reports regarding the primed state of callus and mammalian skin stem cells. As Professor Louis Pasteur famously stated, 'Chance favors the prepared mind.' Similarly, regeneration is more likely to occur in the epigenetically primed state. 

pdf/regeneration-favors-the-epigenetically-primed-state-in-the-19r2umvnx3.pdf

Regeneration favors the epigenetically primed state in the callus or the stem cell

Lysine-Specific Demethylase Epigenetically Regulates Human and Plant Phenomena

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Lysine-Specific Demethylase Epigenetically Regulates Human and Plant Phenomena