Yuichi Harada
National Institutes of Health
6 Papers
59 Citations
Yuichi Harada is an academic researcher from National Institutes of Health. The author has contributed to research in topics: Virus & Influenza vaccine. The author has an hindex of 5, co-authored 6 publications.
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Papers
Anti-influenza activity of phenethylphenylphthalimide analogs derived from thalidomide
Yuma Iwai,Hitoshi Takahashi,Dai Hatakeyama,Kazunori Motoshima,Minoru Ishikawa,Kazuyuki Sugita,Yuichi Hashimoto,Yuichi Harada,Shigeyuki Itamura,Takato Odagiri,Masato Tashiro,Yoshihisa Sei,Kentaro Yamaguchi,Takashi Kuzuhara +13 more
TL;DR: The present results indicate the utility of the PA endonuclease assay in the screening of anti-influenza drugs and are therefore useful for future strategies to develop novel anti-Influenza A drugs and for mapping the function of the influenza A RNA polymerase subunits.
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Influenza Gain-of-Function Experiments: Their Role in Vaccine Virus Recommendation and Pandemic Preparedness
Stacey Schultz-Cherry,Richard J. Webby,Robert G. Webster,Anne Kelso,Ian G. Barr,John W. McCauley,Rod S. Daniels,Dayan Wang,Yuelong Shu,Eri Nobusawa,Shigeyuki Itamura,Masato Tashiro,Yuichi Harada,Shinji Watanabe,Takato Odagiri,Zhiping Ye,Gary Grohmann,Ruth Harvey,Othmar G. Engelhardt,Derek J. Smith,K. Hamilton,Filip Claes,Gwenaelle Dauphin +22 more
TL;DR: Specific examples are provided of how different types of data, including information garnered from GOF studies, have helped to shape the influenza vaccine production process—from selection of candidate vaccine viruses to the manufacture and stockpiling of safe, high-yield prepandemic vaccines for the global community.
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Application of deglycosylation to SDS PAGE analysis improves calibration of influenza antigen standards
Ruth Harvey,Michelle Hamill,James S. Robertson,Philip D. Minor,Galina M. Vodeiko,Jerry P. Weir,Hitoshi Takahashi,Yuichi Harada,Shigeyuki Itamura,Pearl Bamford,Tania Dalla Pozza,Othmar G. Engelhardt +11 more
TL;DR: A collaborative study to assess the effect of adding a deglycosylation step prior to the SDS-PAGE analysis for the assessment of relative HA content found that while the final agreed HA value of the samples tested was not significantly different with or without deglyCosylation, the degly cosyation step greatly improved between-laboratory agreement.
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Systematic evaluation of suspension MDCK cells, adherent MDCK cells, and LLC-MK2 cells for preparing influenza vaccine seed virus
Kazuya Nakamura,Yuichi Harada,Hitoshi Takahashi,Heidi Trusheim,Roth Bernhard,Itsuki Hamamoto,Asumi Hirata-Saito,Teruko Ogane,Katsumi Mizuta,Nami Konomi,Yasushi Konomi,Hideki Asanuma,Takato Odagiri,Masato Tashiro,Norio Yamamoto,Norio Yamamoto +15 more
TL;DR: MDCK-N might be useful for the preparation of influenza vaccine seed viruses, and when applied to passages in hen eggs, A(H1N1), B-Victoria, and B-Yamagata viruses grew well in eggs, while none of the cell isolates of A(3N2) viruses did.
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Comparison of suspension MDCK cells, adherent MDCK cells, and LLC-MK2 cells for selective isolation of influenza viruses to be used as vaccine seeds
Yuichi Harada,Hitoshi Takahashi,Heidi Trusheim,Bernhard Roth,Katsumi Mizuta,Asumi Hirata-Saito,Teruko Ogane,Takato Odagiri,Masato Tashiro,Norio Yamamoto,Norio Yamamoto +10 more
TL;DR: Investigating the patterns of co‐infection in clinical samples and exploring whether cell culture technology can selectively propagate influenza viruses from samples containing other respiratory viruses will help solve the problem of the frequent occurrence of egg adaptation–associated antigenic changes observed in egg‐based vaccines.
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