Yuanyuan Han

TL;DR: In this paper, the authors describe a method for imaging particles and/or cells using flow cytometry, which includes transmitting a light beam at a fluidic channel carrying a fluid sample containing particles; optically encoding scattered or fluorescently-emitted light at a spatial optical filter, the spatial optical filtering system including a surface having a plurality of apertures arranged in a pattern along a transverse direction opposite to particle flow and a longitudinal direction parallel to the particle flow.

TL;DR: This paper uses mathematical algorithms and a specially designed spatial filter as the only hardware needed to give flow cytometers imaging capabilities, and obtains high quality image of fast moving cells in a flow cytometer using photomultiplier tube (PMT) detectors, thus obtaining high throughput in manners fully compatible with existing cytometers.

TL;DR: In this article, the authors present a system, devices and methods for image-based sorting of particles in a flow system, including cells of a flow cytometer and an actuator to direct the particle into one of a plurality of output paths of the particle flow device in real-time.

TL;DR: A 2D image-guided cell sorter and a 3D imaging flow cytometer using fast scanning laser excitation sources feature a cameraless design, which reconstructs cell images from the temporal readout of photomultiplier tubes.