The invention relates to a fruit fly quarantine technology, in particular to a quarantine fruit fly multi-target rapid identification method. The quarantine fruit fly multi-target rapid identification method comprises the following steps: extracting genome DNA of a to-be-detected sample; performing PCR by taking the genome DNA as a template and by using specific primers as shown in SEQ ID NO.1 and SEQ ID NO.2; and detecting the PCR product by gel electrophoresis, wherein the sample belongs to quarantine fruit flies if a 603bp specific band occurs, and the sample does not belong to the quarantine fruit flies if the 603bp specific band does not occur. The quarantine fruit flies relate to anastrepha schiner, bactrocera macquart, carpomya, ceratitis, dacus fabricius and/or rhagoletis; and the specific primers can distinguish the quarantine fruit flies from other tephritidae and diptera close species, have high universality and provide powerful technological support for effectively preventing and controlling introduction and diffusion of the quarantine fruit flies and protecting the ecological safety in China.

Quarantine fruit fly multi-target rapid identification method

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Quarantine fruit fly multi-target rapid identification method