Yu Shi

TL;DR: Researchers developed a single-molecule imaging platform to study nucleosome dynamics and chromatin environment interactions in live cells, revealing differential nucleosome diffusion and packing with chromatin density, and transcription's stabilizing effect on nucleosomes.

TL;DR: SmartLLSM as discussed by the authors is a microscope that incorporates AI-based instrument control to autonomously switch between epifluorescent inverted imaging and lattice light sheet microscopy to detect rare events within a heterogeneous cell population.

TL;DR: Researchers introduce a posterior approach to correct focal plane offsets in lattice light sheet structured illumination microscopy, utilizing residual phase information to retrieve axial offsets and minimize artifacts in reconstructed images.

TL;DR: An imaging platform to simultaneously visualize single protein dynamics together with the local chromatin environment in live cells is developed and reveals that nuclear heterogeneity arises from both active and passive process and highlights the need to account for different organizational principals when modeling different chromatin environments.