Ying Yang
Michigan State University
5 Papers
128 Citations
Ying Yang is an academic researcher from Michigan State University. The author has contributed to research in topics: Mass spectrometry & Cleavage (embryo). The author has an hindex of 5, co-authored 5 publications.
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Papers
A Picomole-Scale Method for Charge Derivatization of Peptides for Sequence Analysis by Mass Spectrometry
TL;DR: Collisionally activated dissociation tandem mass spectrometry of TMPP-Ac-derivatives showed dominant a-type ions, accompanied by d- and c- type ions in some cases, allowing sequence determination to be made in a straightforward manner.
114
Trapping of intermediates during the refolding of recombinant human epidermal growth factor (hEGF) by cyanylation, and subsequent structural elucidation by mass spectrometry.
Jiang Wu,Ying Yang,J. T. Watson +2 more
TL;DR: The procedure of quenching and trapping of disulfide intermediates in acidic solution minimizes sulfhydryl‐disulfide exchange, and therefore provides a good measure of folding kinetics and preservation of intermediate species.
52
Probing the Folding Pathways of Long R3Insulin-like Growth Factor-I (LR3IGF-I) and IGF-I via Capture and Identification of Disulfide Intermediates by Cyanylation Methodology and Mass Spectrometry
TL;DR: Folding pathways of IGF-I and LR3IGF-I are proposed based on the time-dependent distribution and disulfide structure of the corresponding trapped intermediates, which have a Glu3 to Arg3 substitution and a hydrophobic 13-amino acid N-terminal extension.
38
Capture and identification of folding intermediates of cystinyl proteins by cyanylation and mass spectrometry
TL;DR: Trapping folding intermediates of cystinyl proteins by covalent modification of free sulfhydryl groups provides the opportunity for isolation, purification, and structural elucidation of individual species.
17