Deciphering DNA sequences is essential for virtually all branches of biological research. With the advent of capillary electrophoresis (CE)-based Sanger sequencing, scientists gained the ability to elucidate genetic information from any given biological system. This technology has become widely adopted in laboratories around the world, yet has always been hampered by inherent limitations in throughput, scalability, speed, and resolution that often preclude scientists from obtaining the essential information they need for their course of study. To overcome these barriers, an entirely new technology was required—Next-Generation Sequencing (NGS), a fundamentally different approach to sequencing that triggered numerous groundbreaking discoveries and ignited a revolution in genomic science.

Introduction to Next Generation Sequencing Technology

Advancements in Bioinformatics and Role in Marine Genomics and Metagenomics

Next-generation sequencing (NGS) technologies have revolutionized the biosciences and become invaluable to the discovery of gene function and its involvement in disease conditions. The fast pace of innovation in NGS technologies has enabled the production of huge volumes of sequence data at progressively lower cost. However, the increasing throughput combined with the growing accessibility of these technologies poses significant challenges for downstream data analysis. Here, we provide an overview of NGS methods and key secondary analysis pipelines with a focus on technology provided by Illumina. As a case study, we highlight potential applications in cancer research. © 2016 by John Wiley & Sons, Inc.


Keywords:

cloud computing;
Next-Generation Sequencing (NGS);
tumor normal analysis;
Whole-Genome Sequencing (WGS);
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Whole‐Genome Sequencing Analysis Using Next‐Generation Sequencing Data

Identifying a complete genome sequencing of canine parvovirus from red-collared doves in Guangxi, Southern China

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Whole‐Genome Sequencing Analysis Using Next‐Generation Sequencing Data

Identifying a complete genome sequencing of canine parvovirus from red-collared doves in Guangxi, Southern China