15 Papers
4 Citations
Ying Wang is an academic researcher from CAS-MPG Partner Institute for Computational Biology. The author has contributed to research in topics: Biology & Gene. The author has an hindex of 6, co-authored 8 publications.
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Papers
Base editing with a Cpf1–cytidine deaminase fusion
Xiaosa Li,Xiaosa Li,Ying Wang,Yajing Liu,Yajing Liu,Bei Yang,Xiao Wang,Xiao Wang,Jia Wei,Zongyang Lu,Zongyang Lu,Yuxi Zhang,Jing Wu,Xingxu Huang,Li Yang,Jia Chen +15 more
TL;DR: A CRISPR–Cpf1-based BE is developed by fusing the rat cytosine deaminase APOBEC1 to a catalytically inactive version of Lachnospiraceae bacterium Cpf 1, which recognizes a T-rich PAM sequence and catalyzes C-to-T conversion in human cells, while inducing low levels of indels, non-C- to-T substitutions and off-target editing.
392
Nascent Pre-rRNA Sorting via Phase Separation Drives the Assembly of Dense Fibrillar Components in the Human Nucleolus
Run-Wen Yao,Guang Xu,Ying Wang,Lin Shan,Peng-Fei Luan,Yang Wang,Man Wu,Liang-Zhong Yang,Yu-Hang Xing,Li Yang,Li Yang,Ling-Ling Chen,Ling-Ling Chen +12 more
TL;DR: The results unveil FC/DFC ultrastructures in nucleoli and suggest a conceptual framework for considering nascent RNA sorting using multivalent interactions of their binding proteins.
267
Dual base editor catalyzes both cytosine and adenine base conversions in human cells
Zhang Xiaohui,Biyun Zhu,Liang Chen,Ling Xie,Weishi Yu,Ying Wang,Linxi Li,Shuming Yin,Lei Yang,Handan Hu,Honghui Han,Yongmei Li,Liren Wang,Geng Chen,Xueyun Ma,Hongquan Geng,Wanfeng Huang,Xiufeng Pang,Zuozhen Yang,Yuxuan Wu,Stefan Siwko,Ryo Kurita,Yukio Nakamura,Li Yang,Mingyao Liu,Dali Li +25 more
TL;DR: A dual adenine and cytosine base editor (A&C-BEmax) is developed by fusing both deaminases with a Cas9 nickase to achieve C- to-T and A-to-G conversions at the same target site.
248
Efficient base editing in methylated regions with a human APOBEC3A-Cas9 fusion
Xiao Wang,Xiao Wang,Jianan Li,Jianan Li,Ying Wang,Bei Yang,Jia Wei,Jing Wu,Ruixuan Wang,Xingxu Huang,Jia Chen,Li Yang +11 more
TL;DR: It is shown that human APOBEC3A-conjugated BEs and versions the authors engineered to have narrower editing windows can mediate efficient C-to-T base editing in regions with high methylation levels and GpC dinucleotide content.
234
Highly efficient prime editing by introducing same-sense mutations in pegRNA or stabilizing its structure
Xiaosa Li,Lina Zhou,Bao-Qing Gao,Guang-Qing Li,Xiao Wang,Ying Wang,Jia Wei,Wenyan Han,Zixian Wang,Jifang Li,Runze Gao,Jun Zhu,Wenchao Xu,Jing Wu,Bei Yang,Xiao Dan Sun,Liu Yang,Jia Chen +17 more
TL;DR: In this article , the same sense mutations at proper positions in the reverse-transcription template of pegRNA were introduced to increase PE's base-editing efficiency up to 4,976-fold (on average 353-fold).