Yigong Shi
Tsinghua University
268 Papers
1.5K Citations
Yigong Shi is an academic researcher from Tsinghua University. The author has contributed to research in topics: Biology & Small nuclear RNA. The author has an hindex of 106, co-authored 248 publications. Previous affiliations of Yigong Shi include Kettering University & Westlake University.
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Papers
Apoptosome: the cellular engine for the activation of caspase-9.
TL;DR: Characterization of the apoptosome by cryo-EM reveals a wheel-shaped heptameric assembly involving Apaf-1 and cytochrome c, which provides a framework for understanding the activation mechanisms of caspase-9.
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Structural, biochemical, and functional analyses of CED-9 recognition by the proapoptotic proteins EGL-1 and CED-4.
Nieng Yan,Lichuan Gu,David Kokel,Jijie Chai,Wenyu Li,Aidong Han,Lin Chen,Ding Xue,Yigong Shi +8 more
TL;DR: It is demonstrated that the C-terminal half of EGL-1 is necessary and sufficient for binding to CED-9 and for killing cells, and a mechanistic framework for understanding apoptosis activation in C. elegans is provided.
114
How Is Precursor Messenger RNA Spliced by the Spliceosome
TL;DR: The spliceosome is proven to be a protein-orchestrated metalloribozyme and the configuration of the splicing active site are strictly conserved between human and yeast.
112
Site-specific cleavage of dna-rna hybrids by zinc finger/foki cleavage domain fusions
TL;DR: Zinc-finger proteins of the Cys2His2 type bind DNA-RNA hybrids with affinities comparable to those for DNA duplexes and exhibit site-specific cleavage of the DNA strand within DNA- RNA hybrids albeit at a lower efficiency.
111
Mechanistic insights into caspase-9 activation by the structure of the apoptosome holoenzyme
TL;DR: The cryoelectron microscopy structure of an apoptotic holoenzyme and structure-guided biochemical analyses demonstrate that the apoptosome activates caspase-9 at least in part through sequestration of the inhibitory CARD domain.
110