Since the development of methods for inserting and expressing genes in baculoviruses, a line of research has focused on developing recombinant baculoviruses that express insecticidal peptides and proteins. These recombinant viruses have been engineered with the goal of improving their pesticidal potential by shortening the time required for infection to kill or incapacitate insect pests and reducing the quantity of crop damage as a consequence. A wide variety of neurotoxic peptides, proteins that regulate insect physiology, degradative enzymes, and other potentially insecticidal proteins have been evaluated for their capacity to reduce the survival time of baculovirus-infected lepidopteran host larvae. Researchers have investigated the factors involved in the efficient expression and delivery of baculovirus-encoded insecticidal peptides and proteins, with much effort dedicated to identifying ideal promoters for driving transcription and signal peptides that mediate secretion of the expressed target protein. Other factors, particularly translational efficiency of transcripts derived from recombinant insecticidal genes and post-translational folding and processing of insecticidal proteins, remain relatively unexplored. The discovery of RNA interference as a gene-specific regulation mechanism offers a new approach for improvement of baculovirus biopesticidal efficacy through genetic modification.

https://www.mdpi.com/1999-4915/7/1/422/pdf

Expression, Delivery and Function of Insecticidal Proteins Expressed by Recombinant Baculoviruses

Scorpion peptide LqhIT2 activates phenylpropanoid pathways via jasmonate to increase rice resistance to rice leafrollers.

Expression of a neurotoxin gene improves the insecticidal activity of Spodoptera litura nucleopolyhedrovirus (SpltNPV).

Jute hairy caterpillar, Spilosoma (=Spilarctia) obliqua (Walker) (Arctiidae: Lepidoptera), is an irregular and polyphagous insect pest that occurs all over India A severe natural viral epizootic was observed in the field population of S obliqua in jute The virus was characterized as Spilosoma obliqua nucleopolyhedrovirus (SpobNPV NBAIR1) based on morphological, biological, and molecular characterization Under scanning and transmission electron microscopy (SEM and TEM), the occlusion bodies (OBs) of SpobNPV NBAIR1 appeared as both tetrahedral and triangular with the size ranged from 104-172 μm The results on the bioassay studies revealed that the lowest LC50 value of 293 OBs/mm2 by leaf disc bioassay against the second instar S obliqua showed its high virulence The identity of SpobNPV was further confirmed by the amplification of polyhedrin gene (NCBI GenBank accession no MK288145) Eleven species of lepidopteran insects, viz, Spodoptera litura, S exigua, S frugiperda, Helicoverpa armigera, Plutella xylostella, Amsacta albistriga, Maruca vitrata, Trichoplusia ni, Pieris brassicae, Agrotis ipsilon, and Bombyx mori, were not susceptible to the infection with SpobNPV NBAIR1 Field experiments on jute crop revealed 6892, 7859, and 9316% reduction in larval population of S obliqua at 3, 4, and 7 days post spray of SpobNPV NBAIR1 (15 × 1012 OBs/ha at 2 ml/l), respectively The present study is a renewed effort in the management of S obliqua by the new strain of SpobNPV NBAIR1

/pdf/characterization-and-field-evaluation-of-tetrahedral-and-47xn39avi6.pdf

Characterization and field evaluation of tetrahedral and triangular nucleopolyhedrovirus of Spilosoma obliqua (SpobNPV) strain NBAIR1 against jute hairy caterpillar

Biochemical characterization and insecticidal activity of isolated peptides from the venom of the scorpion Centruroides tecomanus

An Buthus martensii Karsch Insect Toxin (BmK IT) gene was inserted into the genome of Autographa californica multicapsid nucleopolyhedrovirus (AcMNPV) to construct a recombinant baculovirus, AcMNPV-BmK IT. The expression of BmK IT was confirmed using RT-PCR, dot blot and SDS-PAGE analysis. Dose-lethal time responses to Spodoptera exigua larvae were compared between wild-type baculovirus AcMNPV and recombinant virus AcMNPV-BmK IT. At the concentration of 1 × 107 PlBs/mL, the median lethal time of recombinant baculovirus (LT50 = 73.6 h) on third instar S. exigua larvae showed an improvement of 13.2% over the efficacy of wild type virus (LT50 = 84.8 h) during a 192 h infection.

http://biotechnology.sxu.edu.cn/docs/20100521095340694028.pdf

Baculovirus-mediated expression of a Chinese scorpion neurotoxin improves insecticidal efficacy

Handle everyday research tasks with reliable, citation-backed results

Your personal Research Agent to handle research tasks with citation-backed results

Popular Tasks used by Researchers

How can I help with your research?

Meet SciSpace

Get more enhanced response by uploading the PDFs you want me to reference.

No relevant PDFs in your library

SciSpace is the AI research assistant for academics. Run systematic literature reviews on 280M+ papers, and write papers with cited sources. Trusted by 1M+ students, PhDs & researchers.

SciSpace | AI for Research

Analyze PDFs

Code & Manuscripts

Funding & Grants

Literature & Patents

Medical & Clinical Data

Systematic Review

Visualize & Present

Web & Data

Build a Google Scholar-like website for your research.

Build a website

Create charts and images for your research

Create a Chart

Write a paper for submission to a journal

Draft a manuscript

Patent Search

Design eye-catching scientific posters in minutes.

Scientific Poster Generation

Systematic Literature Review

One task is running at the moment. Your messages will be shown right after.

Drag and drop or click here to browse

Loved by <highlight>1 million+</highlight> researchers

Extract a list of specific topics and their sources from unstructured text

Topics

Compare and analyze relevant papers that matches with your search

Papers

Get insights from PDFs and bookmarked papers from your library

My library

Recent searches

Try searching for:

Catch AI-generated content in scholarly and non-scholarly content

{ai} Detector

Ai Writer

Get PDF Summaries, highlighted text explanations 

Chat with PDF

Effortlessly create in-text citations and bibliographies in APA and 2,500 other formats

Citation generator

Get explanations, summaries, and answers on academic papers

Ease up your research workflow with {scispace}'s cohort of exciting AI tools

Elevate your academic writing skills and convey your ideas the way you want

Paraphraser

Explore our range of reading and writing tools

Your file is being prepared and should be ready in a few minutes. If it's a large file, it might take a bit longer. You can close this window, and we'll email you the file when it's done.

You have reached a maximum limit of <strong>{limit}</strong> columns in the table. Remove at least <strong>1</strong> column to add or create another one.

Yi Sun

Author Tools

Chat about Author

Papers

Baculovirus-mediated expression of a Chinese scorpion neurotoxin improves insecticidal efficacy