Purification, characterization and antitumor activity of polysaccharides from Pleurotus eryngii residue.

Antitumor activity of a polysaccharide from Pleurotus eryngii on mice bearing renal cancer

Antioxidant, antitumor and immunostimulatory activities of the polypeptide from Pleurotus eryngii mycelium.

Both fresh Codonopsis lanceolata and lactic acid bacteria fermented Codonopsis lanceolata were extracted with water at , and tested for anticancer activity using several human cancer cell lines. The fermented extracts inhibited the growth of hepatocellular carcinoma cells up to 90%, compared to 75% for fresh Codonopsis lanceolata. The extracts of cytotoxicity on human normal lung cells (HEK293) were as low as 15%. Especially, human hepatocellular carcinoma cell were more efficiently inhibited than other cells. This extract also inhibited -glucosidase activity up to 60% at 1.0mg/. This fermented extracts showed the inhibition potency on tyrosinase by 25% at 1.0mg/. From the results, the fermented Codonopsis lanceolata enhanced several biological activities up to , compared to those from fresh Codonopsis lanceolata. It implies that fermentation process could be one of useful methods of utilizing low quality Codonopsis lanceolata. Because this process could yield high amounts of biologically active compounds by the help of microbial growth.

Comparison of Biological Activities of Fermented Codonopsis lanceolata and Fresh Codonopsis lanceolata

Human beings are equipped with antioxidant defense systems to neutralize free radicals as free radicals could damage macromolecules, subsequently resulting in serious diseases. Researchers have been attracted to search for potential natural antioxidants to reduce oxidative damage. Pleurotus and Streptococcus thermophilus have been chosen as sources of sustainable bioactive compounds that have been consumed for thousands of years. Polysaccharides are important bioactive components produced by Pleurotus mushrooms and Streptococcus thermophilus bacteria. Additionally, there is a continued interest in sulfonation of crude polysaccharides from both sources, since sulfonation has been found to improve or create new bioactive properties in polysaccharides. Both crude and sulfated polysaccharides with good antioxidant capacities have great potential for the further development as commercial products. This review focuses on characterization, sulfonation methods, and antioxidant capacity evaluations of polysaccharides from Pleurotus and S. thermophilus. Common antioxidant capacity assays, including the mechanisms underlying each assay, and various experimental procedures are also discussed.

https://onlinelibrary.wiley.com/doi/pdf/10.1111/1541-4337.12252

Sulfonation and Antioxidative Evaluation of Polysaccharides from Pleurotus Mushroom and Streptococcus thermophilus Bacteria: A Review

Effect of the Crude Polysaccharide of Pleurotus eryngii on the Activation of Immune Cells

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Effect of the Crude Polysaccharide of Pleurotus eryngii on the Activation of Immune Cells