The invention discloses a microfluidic chip based fluorescence test method for multiplex nucleic acid amplification products. The microfluidic chip comprises a sample adding hole, an amplification cavity, a siphonage-pipeline, a first interface valve, a predistribution cavity, a second interface valve, test cavities, a liquid waste cavity, a self-venting pipeline and a third interface valve. The microfluidic chip can evenly distribute the multiple amplification products to the different test cavities which are provided with specific probes for sample pre-spotting. The test method can utilize a single fluorescence channel to test the multiple amplification products, thereby reducing the fluorescence channel quantity of an instrument.

Microfluidic chip based fluorescence test method for multiplex nucleic acid amplification products

The invention relates to an optofluidics detector for measuring content of phosphor in seawater by vanadium molybdate yellow spectrophotometry, and belongs to the field of instrument analysis. The optofluidics detector is characterized in that a spectrometric method and an optofluidics chip are combined, and especially, the system is subject to modularizing design; a detector chip comprises a liquid mixing module, a photoelectric detection module and a data analysis module; a to-be-detected liquid and an indication agent flow into the chip through an inlet of the liquid mixing module, and flow out of the chip through the photoelectric detection module, so as to obtain light intensity data, and transmit to the data analysis module. The optofluidics detector has the advantages that by adopting the optofluidics technique, the content of trace phosphate in the seawater is detected by the spectrometric method in a microfluid channel; by adopting the modularizing design, the efficiency and maintenance usability of the whole system are greatly improved.

Optofluidics detector for measuring content of phosphor in seawater by vanadium molybdate yellow spectrophotometry

The invention discloses a textile formaldehyde and pH value detection device and method. The device comprises a control unit, a photoelectric detection light source, a color detection mechanism, a photoelectric detection mechanism and a micro-fluidic chip, wherein the photoelectric detection light source, the color detection mechanism and the photoelectric detection mechanism are electrically connected with the control unit respectively; the micro-fluidic chip is located below the photoelectric detection light source and the color detection mechanism, a formaldehyde detection area of the micro-fluidic chip corresponds to the photoelectric detection light source, and a pH value detection area of the micro-fluidic chip corresponds to the color detection mechanism; the photoelectric detectionmechanism is positioned below the formaldehyde detection area of the micro-fluidic chip. According to the textile formaldehyde and pH value detection device and method provided by the invention, tedious operation and manual errors are avoided, textile formaldehyde and pH value detection is standardized, and the accuracy and timeliness of textile formaldehyde and pH value detection are greatly improved.

Textile formaldehyde and pH value detection device and method

The invention discloses a protein concentration test chip based on a microfluidic technology, which comprises an upper microfluidic chip and a lower microfluidic chip which are arranged in cooperationwith each other and can rotate oppositely around the central position as an axis, wherein a working fluid inlet is arranged on the upper microfluidic chip and a plurality of identical fluid collection pipes are radially arranged around the working fluid inlet, the head of each collection pipe is communicated with the working fluid inlet, reaction cavities are arranged on the lower microfluidic chip at corresponding positions of the ends of the fluid collection pipes, and sample through holes are arranged between the ends of the adjacent two fluid collection pipes respectively; through the test chip disclosed by the invention, the amount of working fluid and test samples used in the traditional BCA protein quantitative analysis process can be reduced to a great extent, steps are simplifies, and sensitivity is high. The invention has the advantages of simple structure, stable performance, low cost, significant progress and excellent popularization and application value.

Protein concentration test chip based on microfluidic technology

A microfluidic apparatus includes a base substrate (300); a microfluidic device (400) on the base substrate (300) and including a plurality of micro fluidic channels (410); a plurality of light sources (200) of different colors respectively emitting light of different wavelength ranges; a light extraction apparatus (1000) for extracting light respectively from the plurality of light sources (200) of different colors and a plurality of photosensors (500). The light extraction apparatus (1000) includes a light guide plate (100) having a plurality of light incident portions (112) for respectively receiving light respectively incident from the plurality of light sources (200) of different colors, the plurality of light incident portions (112) being on a side of the light guide plate (100) away from the microfluidic device (400); and a plurality of light extractors (120) on the light guide plate (100). The microfluidic apparatus can detect multiple substances simultaneously.

Microfluidic apparatus, method of detecting substance in microfluidic apparatus, and light extraction apparatus

The invention provides a multi-sample multi-index micro-fluidic optical detection system which comprises a photoelectric detection module, an intelligentized rotating system and a micro-fluidic chip. The photoelectric detection module consists of LED light sources, light paths, photocell sensors and a photoelectric sensor. The intelligentized rotating system comprises a motor, a mandrel and a drive program. The drive program controls operation of the motor, the motor drives the mandrel to rotate so as to drive the micro-fluidic chip or the LED light sources to rotate. The LED light sources are consistent to micro-fluidic chip detection cells in number and distribution situation. The micro-fluidic chip is disc-shaped and is provided with the transparent detection cells for a biochemical reagent. During application, the intelligentized rotating system drives the micro-fluidic chip or the LED light sources to rotate, enables the detection cells for fixing the specific biochemical reagent to be aligned to the LED light sources identical to the detection cells in optimum absorption wavelength and determines an absorbance value. The multi-sample multi-index micro-fluidic optical detection system has the advantages that one instrument can be applied to detection of a variety of multi-sample multi-detection-index micro-fluidic chips and is high in utilization rate and the like.

Multi-sample multi-index micro-fluidic optical detection system

The utility model provides a disc micro -fluidic chip with remove bubble function, including 1 draw -in groove and at least 1 detecting element of group, detecting element distributes around the centre of a circle, and the shared angle of every group is (img file='DSA0000130427610000011. TIF'wi='105'he='111' /) (n is detecting element's quantity), detecting element includes 1 introduction port, 1 sample cell, 1 at least blow vent, 1 at least reaction tank and 1 detection pond, outwards is introduction port, sample cell, reaction tank and detection pond in proper order from the centre of a circle, reaction tank and 1 blow vent link to each other, and the side that the blow vent is located the reaction tank is close to centre of a circle department to go up and communicate each other with the microchannel between each part. When using, at first being fixed in on chip specific position in advance with reagent, then will waiting to examine liquid joining on chip through the introduction port and react, the bubble process centrifugation that produces among the reaction sequence gets into whole disappearances behind the measuring pond, does not detect the photometry and produces the interference.

Disc micro -fluidic chip with remove bubble function

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Multi-sample multi-index micro-fluidic optical detection system

Disc micro -fluidic chip with remove bubble function