Staphylococcal Poisoning Foodborne Outbreak: Epidemiological Investigation and Strain Genotyping

The invention provides an aptamer nucleic acid probe kit for detecting doxycycline residue as well as a preparation method and application thereof. A nanometer magnetic sphere is an inner core and the outer surface of the nanometer magnetic sphere is bonded with a doxycycline nucleic acid aptamer chain, wherein the doxycycline nucleic acid aptamer chain is 5'-GCATGCCTTAAGCGATCG-N35-CCATATTATAAGGCATGC-3', N represents any one of A, T, C and G, N35 represents that random fragment length comprises 35 basic groups, and the probe is suitable for detection of doxycycline residue in animal body.

Aptamer nucleic acid probe kit for detecting doxycycline residue as well as preparation method and application thereof

The invention discloses aptamer-based rapid kanamycin detection test paper as well as a preparation method and application thereof, and belongs to the field of analytical chemistry as well as safety detection on medicine, environment and food The aptamer-based rapid kanamycin detection test paper comprises a sample pad, a colloidal gold pad, a nitrocellulose membrane, a water absorbing pad, a PVC bottom plate, a detection line and a quality control line By the preparation method, by virtue of the characteristics that gold nanoparticles and silver nanoparticles are easy to prepare and modify and can dually amplify a signal as well as the conformational change of an aptamer, the strength change of an optical signal of the detection line is achieved, and thus detection on kanamycin is achieved; by using a test paper method for detecting the kanamycin, simple, convenient and fast detection is achieved, detection can be performed anytime and anywhere, a result is visualized, the detection time is short, an experimental result can be obtained within 10min by just inserting one end of the sample pad of the test paper into detection liquid, and the detection efficiency can be greatly improved; through a reading of a colloidal gold quantometer, quantitative analysis can be achieved; therefore, the aptamer-based rapid kanamycin detection test paper is of great significance in detection on kanamycin residue in animal-derived food

Aptamer-based rapid kanamycin detection test paper as well as preparation method and application thereof

The invention discloses a carbaryl aptamer, a derivative and an application thereof, a nucleotide sequence of the carbaryl aptamer is shown as CCTGCCACGCTCCGCAAGCTTGGTTTGGTTTCTGCAGCGATTCTTGATCGTGCATGGCTCCTTAGCCTCGTAAGCTTGCCGGCACCCGCATCGt or DNA molecule shown in the nucleotide sequence. The carbaryl aptamer and the derivative can be used for separation and detection analysis of carbaryl. The carbaryl aptamer and the derivative have the advantages of simple preparation step, high efficiency, uniform quality, good stability, short exploitation period, low production cost, simple operation and high security.

Carbaryl aptamer, derivative and application thereof

Staphylococcal food poisoning is one of the most common foodborne diseases worldwide; it results from the ingestion of staphylococcal enterotoxins (SEs) in food, mainly Staphylococcus aureus. This study investigated the statistical relationships among morphological enumerations of food-derived S. aureus and production of SEs using different methodologies. Food samples naturally contaminated with coagulase-positive S. aureus were submitted for enumeration on Baird-Parker (BP) agar, Rabbit Plasma Fibrinogen agar (RPFA), and Petrifilm Staph Express count system (STX), and the morphologically typical colonies were isolated for VIDAS and real-time (RT) PCR tests. RPFA and STX displayed better performance for the enumeration of SE-positive S. aureus when compared with BP, including higher frequencies of SE-positive isolates and better correlation indices between typical and SE-positive counts. Among all the evaluated culture media, no significant difference (P > 0.05) was shown on the frequencies of typical colonies that carried 11 individual se genes. In addition, results for SE identification between VIDAS and RT-PCR assay were unparalleled. These data will be valuable for the selection of methods for inspection of food-derived S. aureus.

Enumeration Methods and Production of Enterotoxins in Food-Derived Staphylococcus aureus

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Carbaryl aptamer, derivative and application thereof

Enumeration Methods and Production of Enterotoxins in Food-Derived Staphylococcus aureus