The invention discloses broad-spectrum disease resisting, growth promotion and stress resisting bacillus capable of preventing and treating tomato gray mold and tomato leaf mold and application of the broad-spectrum disease resisting, growth promotion and stress resisting bacillus. The broad-spectrum disease resisting, growth promotion and stress resisting bacillus is bacillus sp. WXCDD105, and is preserved in China General Microbiological Culture Collection Center (CGMCC); the preservation address is 3, No.1 Yard, Western Beichen Road, Chaoyang District, Beijing, the preservation date is May 25, 2016, and the preservation number is CGMCC No.12496. A biocontrol strain provided by the invention has ultraviolet-resisting and drought-resisting properties, has stronger prevention and treatment effects on the tomato gray mold and the tomato leaf mold, and can inhibit fungal diseases including wilt fusarium of a plurality of types of crops, corn foot root bacteria, rice wilt pathogens, acanthopanax senticosus root rot pathogens, corynespora cassiicolai, colletrichum orbiculare, exserohilum turcicum, tomato spot blight fungi, sunflower sclerotinia sclerotiorum, botrytis cinerea, fusarium oxysporum, verticillium fusarium, alternaria alternata and the like; the broad-spectrum disease resisting, growth promotion and stress resisting bacillus has a growth promotion effect on tomato seeds and seedlings and can also improve the quality of fruits and reduce the rotting rate.

Broad-spectrum disease resisting, growth promotion and stress resisting bacillus capable of preventing and treating tomato gray mold and tomato leaf mold and application of broad-spectrum disease resisting, growth promotion and stress resisting bacillus

The invention provides a carbon-based bio-organic fertilizer and a preparation method thereof, and the carbon-based bio-organic fertilizer is based on biochar and amino acids. Organic materials are uniformly mixed, and inoculated into a microbial fermentation liquid having the functions of promoting growth and antagonizing pathogenic bacteria. The organic materials comprise 20-30% by mass of the biochar, 30-40% by mass of amino acid powder and 40-50% by mass of pig manure rice straw compost, and the quantity of a functional microorganism is 0.5-80 million/g. The carbon-based bio-organic fertilizer prepared by the method can inhibit the amount of soil-borne pathogens in soil, reduces the incidence of the soil-borne diseases in crops, increases the content of effective nutrients in the soil,promotes crop growth, and increases yield.

Carbon-based bio-organic fertilizer and preparation method and application thereof

The invention discloses a cucumber seedling substrate. The cucumber seedling substrate is prepared from, by weight, 1.5-2.5 parts of biogas dregs, 4-6 parts of chicken manure, 35-45 parts of biological carbon inoculant, 1.5-2.5 parts of straw powder and 1 part of perlite. The preparing method comprises the steps of taking components by weight part and mixing evenly the components through a roller. According to the cucumber seedling substrate, no grass carbon is adopted at all, therefore, the problems that grass carbon is expensive and is non-renewable in resource are solved, meanwhile the problem that large biogas residues pollute environment and an environment pollution problem brought by random stacking and arbitrary firing of corn and wheat straw are solved, and the cucumber seedling substrate can effectively maintain living and life environment of people, is beneficial to cycle development of agricultural economy, and has remarkable economic and social benefits. The use of the cucumber seedling substrate can effectively promote the growth of cucumber root systems, meanwhile is beneficial to reducing occurrence of soil-borne diseases, reduces the use of chemical agents, and facilitates the production of green vegetables.

Cucumber seedling substrate and preparing method thereof

The invention discloses a biochar soil improver and an improvement method for an eroded badland. The biochar soil improver disclosed by the invention is prepared by pyrolyzing and carbonizing waste mushroom sticks of seafood mushroom under an oxygen limiting condition to prepare biochar, and meanwhile, adding a compound fertilizer and pig manure. The biochar soil improver is prominent in soil improving effect and good in plant growth condition and can be used as a soil improver, and a novel waste mushroom stick utilization way is provided to be beneficial to the solution of the problem of efficient and environment-friendly recycle of the waste mushroom sticks of the seafood mushroom.

Biochar soil improver and improvement method for eroded badland

The invention discloses a novel strain Y-3 of bacillus amyloliquefaciens and a preparation method and use method of a bacillus amyloliquefaciens Y-3 biological fresh-keeping agent. The preparation method comprises S1, separating bacillus amyloliquefaciens Y-3, S2, activating antagonistic bacteria, S3, inoculating NB fermentation broth with the antagonistic bacteria and carrying out fermentation until the stress-resistant spore rate of 90% or more, S4, crushing bentonite until fineness of less than or equal to 44 microns and carrying out high temperature and high pressure sterilization, and S5,mixing the NB fermentation broth cultured by the bacillus amyloliquefaciens Y-3, bentonite, PEG8000, a detergent and sodium carboxymethylcellulose, carrying out freeze drying, and carrying out crushing until fineness of less than or equal to 44 microns so that biological fresh-keeping wettable powder is obtained. In use, the bacillus amyloliquefaciens Y-3 biological fresh-keeping agent is dilutedwith water and then is applied to picked tomatoes by spraying or soaking. The bacillus amyloliquefaciens Y-3 biological fresh-keeping agent is easy to use and can be conveniently transported transregionally and stored.

Preparation method and use method of bacillus amyloliquefaciens Y-3 biological fresh-keeping agent

The invention relates to a bacillus amyloliquefaciens subspecies WS-8 and application thereof and discloses a bacillus amyloliquefaciens subspecies. The bacillus amyloliquefaciens WS-8 subspecies is preserved in the Common Microorganism Center of the China Committee for Culture Collection of Microorganisms, the serial number is CGMCC No.11787, and the preservation date is December 4th, 2015. The invention further discloses the application of the bacillus amyloliquefaciens subspecies in preventing and treating crop blade surface airborne fungal diseases and soil-borne fungal diseases. Fermentation broth is diluted by 50 folds and is sprayed once every 7-15 days, the prevention effect on tomato leaf mold can reach 72%, and good preventing and treating effects on other airborne and soil-borne fungal diseases are also achieved; meanwhile, the strain can improve the quality of crops, effectively improve the leaf color of plants, increase the content of chlorophyll, improve the disease resistance of the plants, reduce pesticide residues and improve quality. The strain is capable of being conveniently cultured manually and high in breeding speed, facilitates mass production and has the potential to be developed into biopesticide applied in the planting process and posthavesting of vegetables for disease prevention and treatment.

Bacillus amyloliquefaciens WS-8 and application thereof

The invention relates to a microcapsule feed additive, and a preparation method and the application thereof. The microcapsule feed additive comprises the following active ingredients in parts by weight: 5 to 10 parts of benzoic acid or a salt thereof, 2 to 5 parts of organic acid A, 1 to 2 parts of phosphoric acid, and 1 to 10 parts of probiotic bacterial sludge. The feed additive is added into a feed; the content of the benzoic acid or the salt thereof is 200 to 800 mg/kg, and preferably, the concentration is 400 mg/kg. The preparation method for the microcapsule feed additive comprises the following steps: respectively coating probiotics and the acids; mixing a probiotic coating liquid and an acid coating liquid, and performing spray-drying. According to the spray-dried microcapsule feed additive prepared by the invention, integrated spray-drying of the probiotics, sodium benzoate, salts, the organic acid and phosphoric acid is realized, so that the microcapsule feed additive is suitable for scale production; through coating, targeted delivery of the active ingredients is realized; the microcapsule feed additive has the effects of improving intestinal harmful bacteria, improving daily gain, reducing material-to-meat ratio, reducing diarrhea incidence and the like.

Microcapsule feed additive, and preparation method and application thereof

The invention relates to a microbial remediation agent with functions of improving soil and regulating and controlling biotic population structures. The microbial remediation agent comprises a composite functional microorganism, biomass charcoal, mushroom residues and chicken manure. A preparation method of the microbial remediation agent comprises the following steps: mixing the biomass charcoal, the mushroom residues and the chicken manure proportionally; adding composite microorganism dry powder; and after blending uniformly, granulating the mixture by using a granulator to obtain remediation agent granules with the grain size of 2-4 mm. By the use of the bioremediation agent, growth of root systems of crops can be promoted effectively, the microbial flora structure of the soil is improved, the activity of the soil is activated, the activity of soil enzyme is improved, the nutrient of the soil is efficiently transformed and utilized, soil-borne diseases are reduced, and production of green vegetables is facilitated.

Bioremediation agent with functions of improving soil and regulating and controlling biotic population structures and preparation and application thereof

The invention discloses an accelerator for anaerobic fermentation of an antibiotic bacterial residue. The accelerator comprises functional microbes, a microelement and a carrier, wherein the functional microbes are bacillus amyloliquefaciens Y10 and clostridium bifermentans Z-13;charcoal is used as a carrier, the microelement comprises Fe , Co and Ni . The preparation method comprises the steps of mixing various components evenly, drying and then crushing or granulating. The accelerator is used for anaerobic fermentation of a high-protein waste to produce a biogas, especially anaerobic fermentation of the antibiotic antibacterial residue to produce the biogas; organic loads of the antibiotic antibacterial residue can be improved to 4.5-5.5kg/m d from 2.5kg/m d; and the yield of the biogas is improved by more than or equal to 40%.

Accelerator for anaerobic fermentation of antibiotic antibacterial residue and preparation and application of accelerator

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