The invention relates to a method for culturing microalgae by utilizing aquaculture wastewater. The method comprises the following steps: 1) domesticating microalgae: inoculating microalgae into a culture medium for culturing, adding unsterilized aquaculture wastewater in a culture process and gradually increasing the adding rate of the aquaculture wastewater according to the growing number of themicroalgae; 2) activating the microalgae: adding the unsterilized aquaculture wastewater into the culture medium and then inoculating the domesticated microalgae into culture medium for performing amplification culture; and 3) culturing the microalgae: inoculating the activated microalgae into an unsterilized aquaculture wastewater solution and culturing under an outdoor condition, thereby realizing aquaculture wastewater purification and microalgae grease production. According to the method for culturing the microalgae by utilizing the aquaculture wastewater provided by the invention, the unsterilized aquaculture wastewater is adopted for culturing the microalgae under the outdoor condition, the aquaculture wastewater purification and the microalgae grease production can be realized at the same time and the method has the advantages of low cost and suitability for large-scale production.

Method for culturing microalgae by utilizing aquaculture wastewater

The invention discloses a method for large-scale cultivation of nitrogen-fixing cyanobacteria at low cost. The method comprises the following steps: 1, collecting breeding waste of livestock and poultry and performing solid-liquid separation to obtain separated liquid of livestock and poultry breeding waste; 2, determining the contents of total nitrogen and total phosphorus in the separated liquid of the livestock and poultry breeding waste; 3, diluting the separated liquid of the livestock and poultry breeding waste; 4, domesticating and cultivating nitrogen-fixing cyanobacteria and collecting cyanobacterium seeds; 5, preparing a simple medium JYM for nitrogen-fixing Cyanobacteria; 6, mixing the diluted separated liquid of the livestock and poultry breeding waste and the medium JYM according to a volume ratio of 1: 1.5 to 9: 1 and preparing a low-cost nitrogen-fixing cyanobacteriu medium; and 7, inoculating the low-cost nitrogen-fixing cyanobacteriu medium with the domesticated nitrogen-fixing cyanobacteria with OD680 of 0.1 to 0.3 and carrying out natural cultivation. The method of the invention is simple and practicable, has low cost, reasonably utilizes the waste of livestock and poultry industry, and realizes low-cost cultivation of the nitrogen-fixing cyanobacteria.

Method for large-scale cultivation of nitrogen-fixing cyanobacteria at low cost

The invention discloses a method for cultivating microalgae by utilizing biogas slurry. The method comprises the following steps: inoculating the microalgae in an artificial culture medium, harvesting when the specific growth rate of the microalgae begins to decrease, then adding the biogas slurry in batches and harvesting when the specific growth rate of the microalgae decreases to 60-70 percent of the growth rate before the biogas slurry is added for the first time; adding the biogas slurry in batches again and harvesting when the specific growth rate of the microalgae decreases to 50-60 percent of the growth rate before the biogas slurry is added for the first time; adding the biogas slurry at the third time in batches, harvesting when the specific growth rate of the microalgae decreases below 50 percent of the growth rate before the biogas slurry is added for the first time and finishing cultivation. The invention provides the reasonable detailed operation method for realizing microalgae cultivation by utilizing the biogas slurry, which has the benefits that the microalgae yield is improved, the microalgae cultivation cost is reduced, and the gain effect brought by the combination of the microalgae cultivation with other processes is furthest improved.

Method for cultivating microalgae by utilizing biogas slurry

The invention discloses a method for deep sewage treatment by serial microalgae culture. The method includes the steps: 1) microalgae domestication; 2) deep sewage treatment by serial microalgae culture. After anaerobic effluent is treated by the method, degradation rates of COD (chemical oxygen demand), TN (total nitrogen) and TP (total phosphorus) in the anaerobic effluent with the volume fraction of 60% can reach 96%, 94% and 93% respectively, and the degradation rates of the COD, the TN and the TP in the anaerobic effluent with the volume fraction of 40% can reach 95%, 97% and 94% respectively. Experimental results indicate that the treatment effect of serially cultured anaerobic effluent chlorella, spiral seaweeds and scenedesmus for deep treatment of the anaerobic effluent is remarkably better than that of individual use of the anaerobic effluent chlorella, the spiral seaweeds or the scenedesmus for deep treatment of the anaerobic effluent. By the aid of growth characteristic difference and different sewage treatment capacity of different types of microalgae, respective advantages of the microalgae are taken by serial microalgae culture, sewage is deep purified, and novel ideas and methods are provided for sewage treatment.

Method for deep sewage treatment by serial microalgae culture

The invention discloses a method for highly producing microalgae by utilizing biogas slurry for livestock breeding. The method comprises the following steps: (1) reducing the turbidity and the chromaticity and the content of a part of refractory organic matters through flocculating and precipitating; (2) after completing precipitating, carrying out solid-liquid separation through natural settlement; (3) adding a certain amount of sodium carbonate in liquid for regulating the pH (Potential of Hydrogen) and the salinity; (4) inoculating the microalgae in the biogas slurry after expanding culture; (5) carrying out feedback regulation on light intensity and the pH during a culture process; (6) harvesting the microalgae, and completing treatment. According to the method disclosed by the invention, the problem of too low yield of the microalgae in current biogas slurry culture is solved, the harvested microalgae can be used as a raw material of biodiesel, the treated biogas slurry can reachtown secondary sewage emission standard, low emission of the biogas slurry and cyclic utilization of resources are realized, and the method is suitable for large-scale popularization and application.

Method for highly producing microalgae by utilizing biogas slurry for livestock breeding

The invention relates to a method for treating waste water, in particular to a biological treatment method for efficiently purifying biogas slurry in a pig farm through composite microalgae, and belongs to the technical field of biological environmental protection.According to the method, the composite microalgae containing scenedesmus and haematococcaceae are prepared, the biogas slurry in the pig farm is treated through the composite microalgae according to the inoculation amount of 15% to 20% (by volume), the treatment temperature ranges from 24 DEG C to 26 DEG C, the pH value ranges from 7.0 to 8.0, and illumination intensity ranges from 4,000 lux to 6,000 lux.The synergistic effect of two kinds of microalgae is utilized, the defect that the treating effect of a single alga species on the biogas slurry is insufficient is overcome, then the utilization rate of organic matter, ammonia nitrogen, phosphorus and other matter in the biogas slurry in the pig farm increased through the microalgae, and therefore the treating and purifying efficiency of the composite microalgae on the biogas slurry is improved.Compared with treatment of the biogas slurry through a single alga species, the removal efficiency of the composite microalgae on ammonia nitrogen in the biogas slurry is improved by 8%, and the removal efficiency on phosphorus is improved by 15%.According to the method, the method for treating the biogas slurry is provided, the microalgae obtained after treatment can be collected and recycled, ecological treatment of the biogas slurry is achieved, and economic and social benefits are generated while the environment is improved.

Method for efficiently purifying biogas slurry in pig farm through composite microalgae

The invention discloses a composite system used for purifying high ammonia nitrogen pig breeding biogas slurry via algae-bacterium symbiosis/ecological floating bed combined technology. According to the composite system, algae-bacterium symbiosis and ecological floating bed technology is taken as a base, a high-efficiency low-consumption standard treatment system of high ammonia nitrogen pig breeding biogas slurry is constructed, and algae bacterium biomass and plant are obtained at the same time. A purifying method comprises following steps: pig breeding waste water is subjected to solid-liquid separation firstly, and is delivered into an anaerobic fermentation pool; generated high ammonia nitrogen biogas slurry is subjected to pH adjusting and nutritive salt adjusting, and is delivered into the composite system for processing, and biochemical treatment effluent is delivered through a flocculating settling pool and a disinfectant pool so as to realize clear water up-to-standard release. Operation is simple; operation cost is low; pollutant removing efficiency is high; harvested algae bacterium biomass can be taken as a raw material of feed, biological hydrogen production, and biodiesel, and no secondary pollution is caused.

Composite system used for purifying high ammonia nitrogen pig breeding biogas slurry via algae-bacterium symbiosis/ecological floating bed combined technology

The invention discloses a biological treatment method of high-ammonia nitrogen pig raising biogas slurry. The method comprises the following steps: (1) after solid-liquid separation of the pig raising biogas slurry, sequentially performing disinfection, nitrogen-phosphorus ratio adjustment and pH adjustment of a clear liquid to obtain pretreated biogas slurry; (2) inoculating the pretreated biogas slurry with microalgae in a logarithmic phase, wherein the volume ratio of the microalgae to biogas slurry is 5-15%, the treatment temperature is 20-26 DEG C, the illumination intensity is 4,000-6,000lux, and the light-dark ratio is (9-14):(14-9)h; and performing aeration or mechanical stirring for 7-14d; and (3) after the treatment, performing solid-liquid separation of the biogas slurry containing microalgae slurry, wherein the liquid is purified biogas slurry, and the solid is microalgae biomass. The method is simple to operate; the removal rates of ammonia nitrogen and total phosphorus both exceed 90%; the separated microalgae biomass can be used as a raw material of a feed or biodiesel; and secondary pollution is avoided.

Biological treatment method of high-ammonia nitrogen pig raising biogas slurry

The invention relates to a preparation method of a catalyst for catalytic oxidation desulfurization. The method comprises the following steps: grinding medical stone (MFS) original sample into 60-120 meshes, repeatedly washing with deionized water, drying for 4-6 hours at the vacuum of 120 DEG C, then carrying out high-temperature calcination for 1-3 hours in a muffle furnace of 400 DEG C, washing and drying after cooling, soaking the medical stone in 1-2mol/L hydrochloric acid, filtering, drying, soaking modified medical stone in an 15-20% ammonium molybdate solution, stirring for 3-5 hours, and then standing for 6-8 hours, wherein the temperature is kept at 30 DEG C all the time; filtering, drying at the vacuum of 50-80 DEG C until reaching constant weight, finally carrying out high-temperature calcination for 1-2 hours in the muffle furnace of 400 DEG C, and cooling, thus preparing the catalyst Mo/MFS which is good in effect and can be applied to catalytic oxidation desulfurization reaction. The method has the advantages of simplicity, low cost and easily available materials, and the prepared catalyst is good in desulfurization effect and high in repeated utilization factor.

Preparation method of catalyst for catalytic oxidation desulfurization

The invention discloses a biological treatment device and process for high ammonia-nitrogen pig-raising biogas slurry. A body of the biological treatment device is a runway type photo-bioreactor, an efficient and low-consumption standard treatment process for the high ammonia-nitrogen pig-raising biogas slurry is constructed based on a microalgae, photosynthetic bacteria and plant compound ecological system, and meanwhile, algae bacteria biomasses and plants are harvested. The process comprises the following specific steps: pig-raising wastewater is subjected to solid-liquid separation and is introduced into an anaerobic fermentation tank, produced high ammonia-nitrogen biogas slurry is subjected to pH value regulation and nutritive salt regulation and is introduced into the photo-bioreactor to be treated, biochemical effluent is treated by virtue of a flocculation settling tank and a disinfecting tank, and clean water reaches the standard and is discharged. The biological treatment device and the biological treatment process have the advantages that the operation is simple, the removal efficiencies of nitrogen and phosphorus are high, the operation cost is low, the separated algae bacteria biomasses can be taken as the raw materials for feeds, biological hydrogen production or biodiesel, and the secondary pollution is avoided.

Biological treatment device and process for high ammonia-nitrogen pig-raising biogas slurry

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