5 Papers
146 Citations
Yan Xu is an academic researcher from University of South Alabama. The author has contributed to research in topics: Inclusion bodies & Mitochondrial citrate transport. The author has an hindex of 3, co-authored 3 publications.
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Papers
Identification of a Novel Gene Encoding the Yeast Mitochondrial Dicarboxylate Transport Protein via Overexpression, Purification, and Characterization of Its Protein Product
TL;DR: The development of a procedure that enables the expression and isolation of large quantities of functional DTP provides the foundation for comprehensive investigations into the structure/function relationships within this transporter via site-directed mutagenesis, as well as for the initiation of crystallization trials.
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The Yeast Mitochondrial Citrate Transport Protein PROBING THE ROLES OF CYSTEINES, Arg181, AND Arg189 IN TRANSPORTER FUNCTION
Yan Xu,David Kakhniashvili,David A. Gremse,David O. Wood,June A. Mayor,D. Eric Walters,Ronald S. Kaplan +6 more
TL;DR: It is shown that positive charge at these two positions is essential for CTP function, although the chemistry of the guanidinium residue is not, and the hypothesis that the polar face of α-helical transmembrane domain IV, within which Arg181, Arg189, and Cys192 are located, constitutes an essential portion of the citrate translocation pathway through the membrane is led.
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High-Yield Bacterial Expression, Purification, and Functional Reconstitution of the Tricarboxylate Transport Protein from Rat Liver Mitochondria
TL;DR: These studies represent the first overexpression of the rat liver mitochondrial tricarboxylate transporter and will now enable a variety of structural studies, including site-directed mutagenesis, which heretofore could not be performed.
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Developing a dynamic equilibrium system in Escherichia coli to improve the production of recombinant proteins
TL;DR: This study constructed a production system based on a dynamic equilibrium to precisely relieve the host burden and increase the RP production, which can effectively reduce the optimizing costs, and is suitable for developing various types of RP expression hosts rapidly.
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Discovery of N-benzylarylamide derivatives as novel tubulin polymerization inhibitors capable of activating the Hippo pathway.
Jian-Ying Song,Sheng-Hui Wang,Chun Song,Weixing Zhang,Jun Zhu,Xin-Yi Tian,X. Fu,Yan Xu,Cheng-Yun Jin,Sai-Yang Zhang +9 more
TL;DR: In this article , N-benzylarylamide saderivatives were designed and synthesized, and their antiproliferative activities were explored, and 51 target compounds exhibited potent inhibitory activities against MGC-803, HCT-116 and KYSE450 cells with IC50 values in two-digit nanomolar.