24 Papers
19 Citations
Xiaoying Li is an academic researcher from Chinese Academy of Tropical Agricultural Sciences. The author has contributed to research in topics: Papaya ringspot virus & Plasmid. The author has an hindex of 10, co-authored 24 publications.
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Papers
Nimble Cloning: A Simple, Versatile, and Efficient System for Standardized Molecular Cloning.
TL;DR: The cloning technique known as “Nimble Cloning” uses the restriction enzyme, SfiI, in combination with the T5 exonuclease, to linearize the vector and generate 3′-overhangs simultaneously.
High-throughput construction of intron-containing hairpin RNA vectors for RNAi in plants.
TL;DR: A novel restriction-ligation approach that provides a simple but efficient construction of intron-containing hpRNA (ihpRNA) vectors and demonstrates the utility of the ihpRNA constructs generated with pRNAi-GG vector for the effective silencing of various individual endogenous and exogenous marker genes as well as two genes simultaneously.
A cassava common mosaic virus vector for virus-induced gene silencing in cassava
Decai Tuo,Peng Zhou,Pu Yan,Hongguang Cui,Yang Liu,Yang Liu,He Wang,He Wang,Xiukun Yang,Xiukun Yang,Wenbin Liao,Di Sun,Di Sun,Xiaoying Li,Wentao Shen +14 more
TL;DR: This work constructed agroinfection-compatible infectious cDNA clones of cassava common mosaic virus isolate CM that causes systemic infection with mild symptoms in cassava and modified to a viral vector carrying the Nimble cloning frame, which facilitates the rapid and high-throughput cloning of silencing fragments into the viral genome.
Rapid Construction of Stable Infectious Full-Length cDNA Clone of Papaya Leaf Distortion Mosaic Virus Using In-Fusion Cloning.
TL;DR: To the authors' knowledge, this is the first report of the construction of an infectious full-length cDNA clone of PLDMV and the splicing of intron-containing transcripts following mechanical inoculation.
Detection of Papaya leaf distortion mosaic virus by reverse-transcription loop-mediated isothermal amplification
TL;DR: The RT-LAMP method detected specifically PLDMV and was highly sensitive, with a detection limit of 1.32×10(-6) μg of total RNA per reaction, 10 times more sensitive than one-step RT-PCR, while also requiring significantly less time and equipment.
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