The invention discloses a reagent kit and an enzyme-linked immunochromatography for detecting various organophosphorus pesticide residues and particularly relates to the reagent kit and the enzyme-linked immunochromatography for detecting organophosphorus pesticide residues in the production of vegetative agricultural products. The enzyme-linked immunochromatography for detecting the organophosphorus pesticide residues uses the high-sensitivity characteristic of enzyme to carry out detection by adopting the immunochromatography; compared with a traditional enzyme-linked immunoserbent assay (ELISA) for detecting organophosphorus pesticides, the enzyme-linked immunochromatography has the advantages of simplicity and convenience in operation, and visual result, and no special instrument equipment is needed; and compared with a colloidal gold immunochromatography, the enzyme-linked immunochromatography has the advantages of high sensitivity, strong specificity, accuracy in quantification by combining with a readout instrument and the like.

Reagent kit and enzyme-linked immunochromatography for detecting various organophosphorus pesticide residues

Correction test strip for immunochromatographic test strip quantitative detecting instrument, and manufacturing method thereof

The invention relates to a method for testing melatonin. The method comprises the following steps of: 1, extracting a sample to be tested by using a solvent, and extracting the melatonin; 2, filtering and evaporating an extraction solution in the step 1; and 3, adding a detection agent and a buffer solution in the extraction solution evaporated in the step 1, and heating. The method provided by the invention is low in analysis cost without using a precious analytical instrument, strong in specificity and high in sensitivity, and is suitable for testing whether the content of the analytical instrument in medicines and health care products exceeds the standard or not in a field.

Method for testing melatonin

The invention discloses a rapid protein test paper. The test paper comprises a substrate and a chromogenic test paper fixedly arranged on the substrate, and the chromogenic test paper is produced through fully moistening a filter paper in a dipping liquid and through drying; and the dipping liquid includes, by mass, 0.5-10% of an alkali agent, 0.1-0.6% of copper sulfate, 0.3-1.2% of a complexing agent and 1.0-4.0% of a dispersant. The rapid protein test paper has the advantages of simple operation, no need of preparation of a reagent, fast detection speed, suitableness for online detection, solving of the disadvantages of time consumption and labor consumption in detection of proteins by using traditional large apparatuses, simple production, low cost, low detection cost, accuracy and good repeatability of a detection result, and good application prospect.

Rapid protein test paper, and production method and detection method thereof

The invention discloses a method for measuring content of ascorbic acid in plants by utilizing ultraviolet spectroscopy. The method comprises the following steps of: measuring an ultraviolet absorption value of the ascorbic acid in sample extraction buffer, damaging the ascorbic acid so as to measure a background ultraviolet absorption value through an alkali treatment method, and calculating the content of the ascorbic acid through a difference. The method is characterized in that: a high-concentration alkali treatment method is adopted when a background sample is measured, so that the reduced form ascorbic acid is eliminated completely, and the influence of an alkaline environment on ultraviolet absorption of solution to be measured is avoided simultaneously. The method is simple to operate, the measurement result is accurate, and the reducing substances are not easy to get disturbed. The method is suitable for measuring sample ascorbic acid with higher numbers in botanical studies.

Method for measuring content of ascorbic acid in plants by utilizing ultraviolet spectroscopy

The invention discloses a device and a method for detecting Beta-lactamase in raw milk and dairy product. The detecting device comprises a test paper strip and a standard colorimetric card, wherein the standard colorimetric card consists of 9 color blocks that respectively correspond to the following concentrations of the Beta-lactamase from low to high: 0 U/mL, 6 U/mL, 12 U/mL, 25 U/mL, 50 U/mL,100 U/mL, 150 U/mL, 200 U/mL and 250 U/mL; the test paper strip consists of a filter layer, a chromogenic layer, a water absorbing material layer and a substrate and is prepared by taking 2, 2'-biquinoline ethanol solution as a chromogenic reagent and taking CuSO4 as a complexing agent. According to the comparison of the developing degree of the sample to be tested and the standard colorimetric card, the content scope of Beta-lactamase can be quickly judged. The test paper strip is used for quickly detecting the Beta-lactamase content in the raw milk or dairy product, and the method is simple, quick and convenient, has low cost and can be realized and generalized easily.

Device and method for detecting Beta-lactamase in raw milk and dairy product

The invention provides a rapid detection method for Vitamin C content of foods, which is characterized in that: after a sample is pre-treated, a detection sample of 100 mu L is taken to be mixed with a home-made Vitamin C optical detection reagent of 2400 mu L, and Vitamin C in the sample has color reaction with the detection reagent; a reaction product has a maximum absorption peak at 510nm, and a standard curve is worked out by comparison with the Abs510 (absorbance) values of Vitamin C standard samples with different concentrations; and the content of the Vitamin C of the object to be detected can be obtained according to the Abs510 value of the sample to be detected. The detection method has the advantages of short testing time, high accuracy, wide detection range, and the like, and is used for the rapid detection for the Vitamin C of the foods.

Rapid detection method for Vitamin C content of foods

The invention discloses detection test paper for detecting benzoyl peroxide in flour and preparation method of the detection test paper. The test paper comprises a test strip and a standard colourimetric card, wherein the standard colourimetric card comprises 10 color lumps with corresponding concentration gradients of benzoyl peroxide from shallow to deep respectively 0mg/L, 12 mg/L, 36 mg/L, 60mg/L, 84 mg/L, 108 mg/L, 132 mg/L, 156 mg/L, 180 mg/L and 228 mg/L. The test strip comprises a substrate and a color-developing layer adhered on the front end of the substrate, the color-developing layer has a test function, the substrate is made of a PVC (polyvinyl chloride) sheet, and the color-developing layer is prepared by immersing a cellulose acetate film in a color-developing mixed solution consisting of 5-10 percent of potassium iodide, 0.2-0.8 percent of soluble starch and 2-8 percent of sodium carbonate and then drying in vacuum at a temperature of 40-80 DEG C for 20-50min. According to the comparison of the color-developing degree of a sample to be detected and the standard colourimetric card, the content range of the benzoyl peroxide can be rapidly judged. The detection test paper disclosed by the invention is simple, rapid and visual in operation when being used for detecting the benzoyl peroxide remained in the flour, and has high detection sensitivity and accuracy.

Detection test paper for detecting benzoyl peroxide in flour and preparation method of detection test paper

Test strip for measuring protein content of food and testing method thereof

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Papers

Device and method for detecting Beta-lactamase in raw milk and dairy product

Rapid detection method for Vitamin C content of foods

Detection test paper for detecting benzoyl peroxide in flour and preparation method of detection test paper

Test strip for measuring protein content of food and testing method thereof