Xiaojun Wang
Northwestern University
5 Papers
130 Citations
Xiaojun Wang is an academic researcher from Northwestern University. The author has contributed to research in topics: Active site & Adduct. The author has an hindex of 5, co-authored 5 publications. Previous affiliations of Xiaojun Wang include University of California, San Francisco.
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Papers
Evolution of an antibiotic resistance enzyme constrained by stability and activity trade-offs.
TL;DR: Clinically isolated mutants of the beta-lactamase TEM-1 had increased activity against cephalosporin antibiotics but lost both thermodynamic stability and kinetic activity against their ancestral targets, penicillins.
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An ultrahigh resolution structure of TEM-1 beta-lactamase suggests a role for Glu166 as the general base in acylation.
TL;DR: The structure of TEM-1 in complex with an acylation transition-state analogue was determined at ultrahigh resolution (0.85 A) by X-ray crystallography to suggest that Glu166 acts through the catalytic water to activate Ser70 for nucleophilic attack on the beta-lactam ring of the substrate.
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The Structural Bases of Antibiotic Resistance in the Clinically Derived Mutant β-Lactamases TEM-30, TEM-32, and TEM-34
TL;DR: In these three IRT structures, distant substitutions result in accommodations that converge on the same point of action, the local environment of Ser-130, the most pernicious mutants of β-lactamases.
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Noncovalent interaction energies in covalent complexes: TEM-1 beta-lactamase and beta-lactams.
TL;DR: Evaluating noncovalent interactions within covalent complexes by examining the differential stability of TEM‐1 and its inhibitor adducts finds that β‐lactamase‐resistant β‐ lactams moxalactam and imipenem stabilize the enzyme, consistent with the hypothesis.
55
Recognition and resistance in TEM beta-lactamase
TL;DR: Ten transition-state analogues, of greater or lesser similarity to substrates, were tested for inhibition of TEM-1 β-lactamase, the most widespread resistance enzyme to penicillin antibiotics and the most potent inhibitor of the WT enzyme.
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