Seedling method for tissue culture and rapid breeding of camellia semiserrata chi

The invention provides a tea tree tray-soilless seedling substrate, which is formed by mixing tea leaves, turf, pearlite and sands. According to the tea tree tray-soilless seedling substrate provided by the invention, the growth of tea seedling root system can be promoted, the survival rate of tea tree seedling raising by cutting can be increased, the tea seedling growing speed can be accelerated, and the tea seedling survival rate can be remarkably increased; and the cultivated tea seedling has the characteristics of accordant properties, complete root system, robust growth and rich nutritional substrate.

Tea tree tray-soilless seedling substrate

Differential responses of soil nutrients to edaphic properties and microbial attributes following reclamation of abandoned salinized farmland

The invention provides a method for tissue culture and rapid propagation of camellia oleifera The method for tissue culture and rapid propagation of the camellia oleifera comprises the steps of explant sterilization, primary culture, subculture, rooting culture and transplant A preparation method of a rooting medium comprises the steps that a 1/2 MS agar culture medium containing 05mg / L IBA is prepared, wherein agar is 7-10 g / L, and the PH value is 50-55; pulverized perlite is well wrapped by means of a net bag and then is placed into a molten culture medium, the culture medium is fully absorbed by the perlite, then the perlite is drained off slightly and sterilized, and then the rooting medium is obtained According to the method for tissue culture and rapid propagation of the camellia oleifera, the perlite with a certain granularity is used as the culture medium, the culture medium and the agar culture medium are mixed to form the rooting medium, therefore, the root system of the camellia oleifera can breathe fully, the rooting percentage of camellia oleifera cultivation is greatly improved, the root system is developed, the survival rate of transplanting is high, and a technical base is provided for industrialized seedling production and genetic engineering improvement of tissue culture of the camellia oleifera

Method for tissue culture and rapid propagation of camellia oleifera

The invention discloses a method for reducing explant pollution in tea tree tissue culture process. The treatment method comprises the following steps: sterilizing a selected explant, cutting off the discolored cut part of the explant due to sterilization, cutting the part to a 1-1.5 cm long stem section with an axillary bud, inoculating the axillary bud on a culture medium containing antibiotic, inoculating 1-2 stem sections in each bottle, and placing the stem sections in a culture room for culture after being inoculated. The method disclosed by the invention combines ampicillin with streptomycin sulfate to reduce the pollution rate of the explant in the tea tree tissue culture process so as to improve the survival rate.

Method for reducing explant pollution in tea tree tissue culture process

The invention discloses a method for brewing an oil-tea wine by using bio-enzymes. The method comprises the following steps: (1) cleaning and impurity removal; (2) crushing and sieving; (3) mixing; (4) high-temperature enzyme deactivation; (5) multi-enzyme decomposition; (6) carbohydrate supplementation; (7) fermentation; (8) distillation; and (9) aging and packaging. The method disclosed by the invention is simple in process, less in investment, low in energy consumption, and easy to operate. The oil-tea wine prepared by using the method is bright and transparent, mellow and exquisite in taste and typical in style, and has no suspended matters and precipitates.

Method for brewing oil-tea wine by using bio-enzymes

The invention relates to a rapid propagation method for clonal tissue culture of oil tea, comprising four stages of primary generation, secondary generation, rootage and transplantation; wherein the preparation of cutting rootage of tissue culture plantlets and rootage matrix comprises the following steps: the rootage matrix is prepared by yellow-core soil, carbonized rice husk ash and fine sand with the volume ratio of 1:2 to 1: 3; the matrix is put into a cutting container and the tissue culture plantlets are cut in the container and sealed by a transparent film. The rootage and transplantation of the tissue culture plantlets are integrated into a whole body; after 30 to 40 days, the tissue culture plantlets take roots and form complete plants, therefore, the section of transplantation of the tissue culture plantlets is saved and the rootage rate and survival rate are more than 87 percent.

Rapid propagation method for clonal tissue culture of oil tea

The invention provides a process for quickly propagating a large quantity complete oil tea clone plants with roots by tissue culture, which comprises the steps of secondary culture medium screening and hormone combination, seedling strengthening culture medium screening and hormone combination, root preprocessing, and rooting culture medium screening and hormone combination, wherein WPM+BA (Woody Plant Medium and Benzyladenine, 3-7mg/L), IBA (Indol Butyric Acid, 0.1-2mg/L) and ZT (Zeatin, 0.05-2mg/L) are used as secondary culture media for propagation, the propagation period is shortened to 30-40 days, and the propagation coefficient reaches 4.2-9.1; WPM+IBA (0.1-2mg/L) and biotin (0.5-3mg/L) are used as seedling strengthening culture media for strengthening seedlings, and seedlings can take root after 20-25 days; and after being treated with IBA, tissue culture seedlings which are 2-4cm in height are directly inoculated on the culture media of MS (Murashige and Skoog medium, 1/4-1) and AC (Activated Carbon, 200-600), the tissue culture seedlings take root and form complete plants after 25-3 0days, and the rooting rate reaches more than 85%.

Efficient rooting method for oil tea clone tissue culture seedlings

Method for detoxifying clonal tissue culture explant of Camellia oleifera Abel

Introduction Global climate change can affect the sensitivity of tree radial growth to climate factors, but the specific responses of tree radial growth to microclimate along the altitudinal gradient in the long term are still unclear. Methods In this study, the tree-ring width chronologies of Pinus tabuliformis Carr. in Shanxi Province of China were studied at three altitude gradients (1200-1300 m (low altitude), 1300-1400 m (medium altitude) and 1400-1500 m (high altitude)) during 1958-2017. Results The results showed that (1) the climate background could be divided into two periods based on the Mann-Kendall test analysis: 1958–1996 was a stable period (mean annual temperature (MAT)=10.25°C, mean annual precipitation (MAP)=614.39 mm), and 1997–2017 was a rapid change period (MAT=10.91°C, MAP=564.70 mm), indicating a warming and drying trend in the study region. (2) The radial growth of P. tabuliformis at different altitudes showed inconsistent variation patterns. The tree radial growth at low and medium altitudes (CV=27.01% for low altitude and CV=24.69% for medium altitude) showed larger variation amplitudes during the rapid change period than that in the stable period (CV=12.40% for low altitude and CV=18.42% for medium altitude). In contrast to the increasing trend, the tree radial growth rates at the high altitude showed a decreasing trend across years. (3) In the stable period, the radial growth of P. tabuliformis at the low altitude showed a significantly negative response to temperature and a positive response to precipitation in May and June. The tree radial growth at the medium altitude was positively related to precipitation in June and minimum temperature in February. The tree growth at the high altitude was mainly positively correlated with the temperature in May and August. In the rapid change period, the radial growth of P. tabuliformis at the low altitude was affected by more meteorological factors than that in the stable period. Medium-altitude trees were positively influenced by precipitation in June and minimum temperature in January, whereas high-altitude trees responded positively to wind speed in February. (4) Along altitudinal gradients, tree radial growth was more related to temperature than precipitation in the stable period. The tree radial growth at the high altitude during the rapid change period was only affected by wind speed in February, whereas the tree radial growth at low and medium altitudes was mainly affected by temperature to a similar extent during the two periods. Discussion The study indicated that tree growth-climate response models could help deeply understand the impact of climate change on tree growth adaptation and would be beneficial for developing sustainable management policies for forest ecosystems in the transition zone from warm-temperate to subtropical climates.

/pdf/dendroclimatic-response-of-pinus-tabuliformis-carr-along-an-2qavmeec.pdf

Dendroclimatic response of Pinus tabuliformis Carr. along an altitudinal gradient in the warm temperate region of China

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