A rapid resolution liquid chromatography coupled with quadrupole-time-o-flight mass spectrometric (RRLC-Q-TOF-MS) method was established and optimized for the analysis of pharmacokinetic behavior of ginsenoside Rb2 in rats by intravenous injection administration. The metabolism of ginsenosides Rb2 in vivo rat was also explored. In the experiment, Agilent SB C-18 column was selected for the sample separation with 0. 1% aqueous formic acid solution as mobile phase (A) and acetonitrile as mobile phase (B) at a flow rate of 0. 2 mL/min, and the injection volume was set to 5 mu L. Q-TOF-MS was carried out in electron pray ionization (ESI) negative ion mode. The limit of quantification (LOQ, S/N = 10) and limit of detection (LOD, S/N = 3) were 0. 10 and 0. 08. mu g/mL, respectively, and the linear range was 0. 1-1. 26. mu g/mL. The experiment results showed that the concentration. time profile of ginsenoside Rb2 conformed to a two. compartment pharmacokinetic model after intravenous administration for rats. The mean plasma elimination half. lives were (23. 58 +/- 1. 10) min (t(1/2 alpha)), (1306. 55 +/- 147. 23) min (t(1/2 alpha)) for Rb2. By analyzing the urine of rats after intravenous administration and the fecal samples after oral administration of ginsenoside Rb2, it was found that the metabolites were M6, M2(CY), F2, and C-K.

Pharmacokinetic And Metabolic Studies Of Ginsenoside Rb2 In Rats

In this study, a simple and rapid ultra-fast liquid chromatography tandem mass spectrometry method was established and validated to determine ginsenosides Rb2 in rat plasma. Acetonitrile-mediated protein precipitant was applied to the sample preparation. Chromatographic separation was carried out on an Acquity UPLC HSS T3 column (100 × 2.1 mm, 1.8 μm). The analytes were monitored using multiple reactions monitoring mode with precursor-to-product ion transitions at m/z 1077.4-945.3 and m/z 799.8 → 637.8 for ginsenoside Rb2 and internal standard, respectively. The mobile phase was composed of 0.1% formic acid aqueous solution and acetonitrile. The assay showed excellent linearity over the concentration range of 2-1,000 ng/ml, with correlation coefficient >0.995. The method was further validated for selectivity, precision, accuracy, recovery, and stability according to the US Food and Drug Administration guidelines. The validated method was successfully applied to pharmacokinetic and bioavailability studies of ginsenoside Rb2 in rat plasma. Based on the pharmacokinetic results, ginsenoside Rb2 showed slow clearance and low oral bioavailability (0.15%). In addition, the metabolites of ginsenoside Rb2 in rat urine and feces were characterized according to their accurate masses and fragment ions. The proposed metabolic pathway was deglycosylation.

Pharmacokinetic and metabolism study of ginsenoside Rb2 in rat by liquid chromatography combined with electrospray ionization tandem mass spectrometry

Protective Effects of Ginsenosides Rb2 on Irradiation-Induced Hematopoietic System Injury in the Mice

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Protective Effects of Ginsenosides Rb2 on Irradiation-Induced Hematopoietic System Injury in the Mice