Xiangde Ke
8 Papers
20 Citations
Xiangde Ke is an academic researcher. The author has contributed to research in topics: Medicine & Biology. The author has an hindex of 1, co-authored 1 publications.
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Papers
Diversity and evolutionary relationship of nucleotide binding site-encoding disease-resistance gene analogues in sweet potato (Ipomoea batatas Lam.).
TL;DR: The data obtained from sweet potato suggest that the evolution of NBS-encoding sequences in sweet potato occur by the gradual accumulation of mutations leading to purifying selection and slow rates of divergence within distinct R-gene families.
20
Engineering of succinyl-CoA metabolism in view of succinylation regulation to improve the erythromycin production
TL;DR: This work expands the knowledge of succinylation regulation in the TCA cycle, but also validates that CRISPRi is an efficient strategy on the metabolic engineering of S. erythraea.
11
Ammonium sulfate supplementation enhances erythromycin biosynthesis by augmenting intracellular metabolism and precursor supply in Saccharopolyspora erythraea.
TL;DR: Targeted metabolomics analysis uncovered that the addition of ammonium sulfate during the late stage of fermentation resulted in an augmented intracellular amino acid metabolism pool, guaranteeing an ample supply of precursors for organic acids and coenzyme A-related compounds.
3
Enhancing erythromycin production in Saccharopolyspora erythraea through rational engineering and fermentation refinement: A Design-Build-Test-Learn approach.
Minghao Shao,Xiangde Ke,Ju Chu +2 more
TL;DR: The DBTL approach successfully enhanced erythromycin production in S. erythraea CS, achieving a 43.5% increase in yield. Improved precursor synthesis, increased NADPH availability, and altered central carbon metabolism contributed to the increased erythromycin production.
1
An inducible CRISPRi circuit for tunable dynamic regulation of gene expression in Saccharopolyspora erythraea.
Xing Jiang,Xiangde Ke,Xiwei Tian,Ju Chu +3 more
TL;DR: The effectiveness of an inducible gene downregulation approach using CRISPRi and a cumate-inducible promoter is demonstrated, providing valuable insights for optimizing natural product production in actinomyces.