5 Papers
2 Citations
Xi Chen is an academic researcher from University of Southern California. The author has contributed to research in topics: microRNA & Embryonic stem cell. The author has an hindex of 3, co-authored 3 publications.
Chat about Author
Papers
Long-term self-renewal of naïve neural stem cells in a defined condition.
TL;DR: This culture condition provides a robust platform for further dissection of the NSC self-renewal mechanism and promotes potential applications of NSCs for cell-based therapy on nervous system disorders.
5
Optimization of Cas9 RNA sequence to reduce its unexpected effects as a microRNA sponge
Junfeng Jiang,Tao Zeng,Li Zhang,Xingfei Fan,Qishu Jin,Hai-Lai Ni,Yusheng Ye,Lipeng Cheng,Li Li,Liujun Wang,Shaokun Xu,Yu Yang,Juan Gu,Bing Guo,Lei Wang,Xin Li,Yingyi Qin,Jiaxin Li,Jinjiang Wang,Xi Chen,Min Shun Wu,Qi-Long Ying,Xingjun Qin,Yefei Wang,Yue Wang +24 more
TL;DR: In this article , RNA sequence optimization of Cas9 by synonymous mutation improves its safety, and Cas9 RNA functions as a miRNA sponge, which is the dominant regulated miRNA.
2
Optimization of Cas9 RNA sequence to reduce its unexpected effects as a microRNA sponge
Junfeng Jiang,Tao Zeng,Li Zhang,Xingfei Fan,Qishu Jin,Hai-Lai Ni,Yusheng Ye,Lipeng Cheng,Li Li,Liujun Wang,Shan Xu,Yu Yang,Juan Gu,Bing Guo,Lei Wang,Xin Li,Yingyi Qin,Jiaxin Li,Jinjiang Wang,Xi Chen,Min Shun Wu,Qi-Long Ying,Xingjun Qin,Yefei Wang,Yue Wang +24 more
TL;DR: In this article , RNA sequence optimization of Cas9 by synonymous mutation improves its safety, and Cas9 RNA functions as a miRNA sponge, which is the dominant regulated miRNA.
A Chemical-Genetic Approach Reveals the Distinct Roles of GSK3α and GSK3β in Regulating Embryonic Stem Cell Fate.
Xi Chen,Ruizhe Wang,Xu Liu,Yongming Wu,Tao Zhou,Yujia Yang,Andrew A. Perez,Ying-Chu Chen,Liang Hu,Jean Paul Chadarevian,Amir Assadieskandar,Chao Zhang,Qi-Long Ying +12 more
TL;DR: It is shown that GSK3α and GSK2β can be selectively inhibited in mouse embryonic stem cells (ESCs) using a chemical-genetic approach, and this study opens new avenues for defining G SK3 isozyme-specific functions in various cellular processes.
Induction of site-specific chromosomal translocations in embryonic stem cells by CRISPR/Cas9
Junfeng Jiang,Zhang Li,Xingliang Zhou,Xi Chen,Guanyi Huang,Fengsheng Li,Ruizhe Wang,Nancy Wu,Youzhen Yan,Chang Tong,Sankalp Srivastava,Yue Wang,Houqi Liu,Qi-Long Ying +13 more
TL;DR: It is shown that site-specific chromosomal translocations can be generated in mouse embryonic stem cells (mESCs) via CRISPR/Cas9, and chimeric mice can begenerated by injecting these mESCs into host blastocysts.