Werner Klipp
Bielefeld University
40 Papers
839 Citations
Werner Klipp is an academic researcher from Bielefeld University. The author has contributed to research in topics: Rhodobacter & Gene. The author has an hindex of 28, co-authored 39 publications.
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Papers
New derivatives of transposon Tn5 suitable for mobilization of replicons, generation of operon fusions and induction of genes in gram-negative bacteria.
TL;DR: Three types of new variants of the broad-host-range transposon Tn5 are described, which allow the constitutive expression of downstream genes and can be used to generate transcriptional fusions upon insertion, thus facilitating accurate determinations of gene expression.
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Nucleotide sequence of a 24,206-base-pair DNA fragment carrying the entire nitrogen fixation gene cluster of Klebsiella pneumoniae☆
TL;DR: A high degree of homology between the two gene products involved in iron-molybdenum cofactor biosynthesis and the two nitrogenase component I structural proteins (NifD and NifK) was found.
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Identification and mapping of nitrogen fixation genes of Rhodobacter capsulatus: duplication of a nifA-nifB region.
TL;DR: In this article, the Tn5 insertion sites of 30 Nif- mutants were mapped within three unlinked chromosomal regions designated A, B, and C. Hybridization experiments using all 17 Klebsiella pneumoniae nif genes individually as probes revealed homology to nifE, nifS, NifA, and nifB in nif region A.
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Genetic characterization and sequence analysis of the duplicated nifA/nifB gene region of Rhodobacter capsulatus.
TL;DR: A DNA region showing homology to Klebsiella pneumoniae nifA and nifB is duplicated in Rhodobacter capsulatus, and deletion mutagenesis demonstrated that either of the two copies is sufficient for growth in nitrogen-free medium.
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•Journal Article
Regulation of nitrogen fixation in the phototrophic purple bacterium Rhodobacter capsulatus.
Bernd Masepohl,Thomas Drepper,Annette Paschen,Silke Gross,Alice Pawlowski,Karsten Raabe,Kai-Uwe Riedel,Werner Klipp +7 more
TL;DR: In a glnB/glnK double mutant ammonium control of the molybdenum nitrogenase is completely relieved, leading to synthesis of active nitrogenase in the presence of high concentrations of ammonium.
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