The invention relates to an integrated nucleic acid extraction and amplification detection system. The system comprises disposable microfluidic chip and a matched detection apparatus. The disposable microfluidic chip comprises a reaction unit and a waste liquid memory unit, when the disposable microfluidic chip is operated, mutual mixing between the reaction reagents as well as between the reaction reagent and magnetic beads can be realized by means of a vibrating module, depending on a magnetic bead driving module, steps of nucleic acid adsorption and purification can be completed in order; the magnetic bead can be flushed by a PCR amplification reagent through a fluid drive module, an amplification template on the magnetic bead can be eluted, the magnetic bead is introduced into a convection PCR reactor constructed by a vertical channel, and a CPCR amplification reaction and detection are realized through a heating module and a fluorescence acquisition module. The apparatus can integrate nucleic acid extraction and CPCR amplification with detection mutually, nucleic acid extraction and real-time CPCR amplification and detection can be automatically completed on one chip, nucleic acid diagnosis and analysis efficiency can be obviously increased, and the apparatus has the characteristics of small volume, high automation degree, convenience and rapidity, simple operation, and low cost.

Integrated nucleic acid extraction and amplification detection system

The present invention discloses a high-flux nucleic acid analysis apparatus based on a microfluidic chip The high-flux nucleic acid analysis apparatus comprises a microfluidic chip, an extraction unit, a heating unit and a detection unit According to the present invention, under the assisting of the extraction unit, nucleic acid in a cleaved sample is captured by using the AOM membrane in the microfluidic chip, the AOM membrane is washed by using a purification and washing reagent so as to purify the nucleic acid, the nucleic acid template is retained on the AOM membrane, and the heating unit supplies stable reaction temperatures for the upper end and the lower end of a reaction chamber to make the nucleic acid template on the AOM membrane participate into a convection PCR reaction so as to achieve the integration of the nucleic acid extraction and the nucleic acid amplification; the detection unit comprises an LED lamp and a camera, wherein the LED lamp excites the fluorescent dye in the reaction reagent, and the fluorescence signals of a plurality of reaction hole positions are simultaneous detected through the camera; and the system has advantages of low complexity, simple operation and short detection time, integrates the nucleic acid extraction, the nucleic acid amplification and the nucleic acid detection, and can improve the nucleic acid diagnosis and analysis efficiency

High-flux nucleic acid analysis apparatus based on microfluidic chip

The invention discloses a portable isothermal nucleic acid analysis device based on mobile phone detection. A lower isothermal heating unit is arranged at the bottom of a reaction reagent and is used for heating the reaction reagent; an upper constant-temperature unit is arranged at the top of the reaction reagent and is used for heating the reaction reagent; a heat capacity regulating unit is arranged between the lower isothermal heating unit and the upper constant-temperature unit, and the lower isothermal heating unit and the upper constant-temperature unit are kept closely attached; a working angle mechanical regulating unit comprises an optocoupler at the bottom and a stepping motor; a location origin is found out by virtue of the optocoupler; and by virtue of the stepping motor, the lower isothermal heating unit, the reaction reagent, the upper constant-temperature unit and the heat capacity regulating unit are driven to move. A mobile phone is located on the side face of the reaction reagent and is used for conducting fluorescence detection on the reaction reagent. The portable isothermal nucleic acid analysis device disclosed by the invention can effectively simplify the complexity thereof, shorten the cost of nucleic acid analysis and reduce the cost thereof, so that portable, mobile and rapid on-site molecular diagnosis is achieved.

Portable isothermal nucleic acid analysis device based on mobile phone detection

The invention relates to a fluorescence imaging device and method. The device comprises at least one group of laser sources, a to-be-tested sample, an optical color filter, an imaging lens, an image sensor and an intelligent terminal. An optical filter is positioned above the to-be-tested sample and fixedly arranged on the lower end of the imaging lens, the image sensor is fixedly arranged on the upper end of the imaging lens and connected with the intelligent terminal; the laser sources emit laser to be irradiated onto the to-be-tested sample and generate fluorescence which is irradiated onto the optical color filter, only fluorescence with specific wavelength in the fluorescence can penetrate through the optical color filter and irradiate into the imaging lens, and is focused on the image sensor by the imaging lens. According to the fluorescence imaging device, the image acquiring speed and the irradiation uniformity of the laser excited light can be improved, the stability and reliability of equipment can be improved, the cost can be effectively lowered, the utilization rate of the light source can be increased, and the heat radiation characteristic can be greatly improved.

Fluorescence imaging device and method

The invention discloses a handheld fluoroimmunoassay device The device comprises a shell and an upper cover, the shell and the upper cover enclose an accommodating chamber, a fluorescence data acquisition module, an photoelectric scanning module and a master control module are arranged in the accommodating chamber, and the surface of the upper cover is provided with a touch screen; the fluorescence data acquisition module is arranged above the photoelectric scanning module, and the master control module is respectively connected with the fluorescence data acquisition module, the photoelectricscanning module and the touch screen; the device also comprises a pedestal bottom with a reagent card slot integrated to the photoelectric scanning module; and the position of the fluorescence data acquisition module in the accommodating chamber is moving The fluorescence data acquisition module includes a plurality of excitation light sources which are light sources having different wavebands respectively, so multi-purpose use of one machine is realized, the detection of multiple fluorescent band samples can be achieved, and the application range of the device is enlarged; and the device adopting the moving fluorescence data acquisition module and fixed reagent card structure has the advantages of compact integral structure layout, small size, and easiness in realization of handheld operation and carrying

Handheld fluoroimmunoassay device

The invention discloses an automatic immunodetection micro-fluidic chip fluorescent quantification detection device which comprises an excitation light source, an exciting light focusing module, a fluorescence collection module, a photoelectric conversion module, a linear motion platform module, a circuit module, an automatic reaction execution module and a waste reaction liquid removal module In the detection process, a chip is subjected to one-dimensional quantitative scanning detection through linear light spots obtained by the exciting light focusing module; a controller obtains the concentration or cell number of a sample to be detected based on fluorescence signal intensity distribution information in a chip two-dimensional rule detection area; the detection result is connected with a mobile phone through Bluetooth and is transmitted to medical institutions in real time, and remote diagnosis or epidemic disease distribution area monitoring is realized A linear light spot one-dimensional scanning detection method is adopted, and quantification of micro-fluidic chip detection is realized Moreover, by means of the automatic reaction execution module, automation of the micro-fluidic chip detection is realized; due to the integrated waste reaction liquid removal module, the allowed reaction sample amount is increased, and the detection sensitivity is improved

Automatic immunodetection micro-fluidic chip fluorescent quantification detection device

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Automatic immunodetection micro-fluidic chip fluorescent quantification detection device