Watchalee Chuenchor
National Institutes of Health
14 Papers
62 Citations
Watchalee Chuenchor is an academic researcher from National Institutes of Health. The author has contributed to research in topics: Chemistry & Active site. The author has an hindex of 11, co-authored 13 publications. Previous affiliations of Watchalee Chuenchor include University of Maryland, College Park & Suranaree University of Technology.
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Papers
Design of an expression system to enhance MBP-mediated crystallization
Tengchuan Jin,Watchalee Chuenchor,Jiansheng Jiang,J.-G. Cheng,Yajuan Li,Kang Fang,Mo Huang,Patrick F. Smith,Tsan Sam Xiao +8 more
TL;DR: The successful crystallization of all of the targets demonstrates the validity of the approach and expands the arsenal of the crystallization chaperone toolkit, which may be applicable to crystallized of other difficult protein targets, as well as to other crystallizationChaperones.
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A Switch between One- and Two-electron Chemistry of the Human Flavoprotein Iodotyrosine Deiodinase Is Controlled by Substrate
TL;DR: Crystallographic, pH, and redox studies demonstrate the role of substrate in organizing the active site for effective catalysis, and the pH dependence of IYD binding and turnover supports the importance of direct coordination between substrate and FMN for productive catalysis.
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Enzymatic synthesis of cello-oligosaccharides by rice BGlu1 β-glucosidase glycosynthase mutants
Greanggrai Hommalai,Stephen G. Withers,Watchalee Chuenchor,James R. Ketudat Cairns,Jisnuson Svasti +4 more
TL;DR: The BGlu1 E414G glycosynthase was found to prefer longer chain length oligosaccharides that occupy at least three sugar residue-binding subsites as acceptors for productive transglucosylation, which is the first report of a beta-glucansynthases derived from an exoglycosidase that can produce long-chain cello-oligosACcharides.
Two Distinct Binding Modes Define the Interaction of Brox with the C-Terminal Tails of CHMP5 and CHMP4B.
Ruiling Mu,Vincent Dussupt,Jiansheng Jiang,Paola Sette,Victoria Rudd,Watchalee Chuenchor,Nana F. Bello,Fadila Bouamr,Tsan Sam Xiao +8 more
TL;DR: It is demonstrated that hydrophobic residues at the CHMP4B C-terminal amphipathic α helix bind a concave surface of Brox, a mammalian paralog of Alix, and insights are provided into the overlapping but distinct binding profiles of ESCRT-III and the Bro1 domain proteins.
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Assembly Pathway and Characterization of the RAG1/2-DNA Paired and Signal-end Complexes *
TL;DR: It is found that RAG1/2 binds only a single 12RSS or 23RSS and reserves the second DNA-binding site specifically for the complementary RSS, to form a paired complex that reflects the known 12/23 rule of V(D)J recombination.
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