Xanthan-chitosan hydrogels have been rheologically characterized in the simulated gastrointestinal conditions while the studies on the applicability of the system for anaerobic bacterial Bifidobacterium BB01 encapsulation in yogurt are scarce. The aim of this work was to investigate the effect of a new encapsulation system (xanthan-chitosan-xanthan) on the viability of B. bifidum BB01 in yogurt during 21 days storage at 4 °C and 25 °C, respectively. Chitosan was applied as an inner layer to coat the xanthan-probiotic and xanthan was again used as an outer layer for coating chitosan-xanthan-probiotic particles. Moreover, the probiotic survival under the simulated gastrointestinal conditions and bile salt solution were investigated. The results indicated that xanthan-chitosan-xanthan microcapsules (XCX) and xanthan-chitosan microcapsules (XC) significantly (p

Effect of xanthan-chitosan-xanthan double layer encapsulation on survival of Bifidobacterium BB01 in simulated gastrointestinal conditions, bile salt solution and yogurt

The invention discloses a probiotic microcapsule added with prebiotics; the probiotic microcapsule is formed by coating a core material with a wall material; the wall material is a mixture of sodium alginate and protein wall material in the proportion of 2:1; the final concentration is 3-5%; and the protein wall material is soyabean protein or whey protein. The invention further discloses a preparation method of the probiotic microcapsule added with the prebiotics. According to the invention, probiotics are coated with the wall material; sodium alginate is capable of increasing the endurance capacity of the probiotics to pepsase and the like; the protein wall material is capable of increasing the endurance capacity of the probiotics to gastric acid and the like; by means of combination of sodium alginate and the protein wall material, the number of living bacteria entering an intestinal tract is greatly increased; skim milk powder in the core material is capable of increasing the endurance capacity of the probiotics to the environment; loss of the probiotics in producing, processing, transporting and storing processes is reduced; the prebiotics in the core material are capable of stimulating growth of beneficial bacteria; and the probiotic microcapsule disclosed by the invention is capable of improving the microenvironment of a host intestinal tract and increasing the disease resistance of hosts.

Probiotic microcapsule added with prebiotics and preparation method thereof

The invention relates to a microencapsulated embedded probiotic and a processing method thereof. The microencapsulated probiotic mainly comprises a wall material and a core material, wherein the wall material embeds the core material to form a microcapsule and the core material is a probiotic. The processing method includes dissolving the probiotic into water, adding the wall material, performing high-pressure homogenization to allow the probiotic and the wall material to form a fully uniform emulsion, and drying the emulsion until the water content is lower than 5% to form the microcapsule. The probiotic is a probiotic easy in moisture absorption and/or non-resistant to heat. Compared with un-embedded probiotics, the microencapsulated embedded probiotic is obviously improved in moisture absorptivity and heat resistance. The probiotic is convenient in storage, transportation and using. In particular, after the heat resistance is improved, the probiotic can be provided to human bodies through food cooking.

A probiotic and a processing method thereof

Abstract The effects of cell suspension-alginate ratios, sodium erythorbate, and inulin on encapsulation yield of microcapsules of Bifidobacterium bifidum BB01 were studied by Box-Behnken design. The experimental results indicated that cell suspension-alginate ratios, sodium erythorbate and inulin had a significant impact on encapsulation yield, and the embedding yield could be enhanced significantly in the condition of 1:3 cell suspension-alginate ratios, 0.12% sodium erythorbate, and 6% inulin. The optimal embedding yields of microencapsulation of B. bifidum BB01 were observed to be 81.52%, that values were very close to the expected values 81.81%, so the method was effective.

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Optimization the Process of Microencapsulation of Bifidobacterium bifidum BB01 by Box-Behnken Design

The invention discloses a preparation method of probiotic microcapsules. The preparation method comprises the following steps: 1) preparing a mixed probiotic preparation; 2) preparing a traditional Chinese medicine extract; 3) homogenizing; and 4) drying: treating the emulsified liquid collected in the step 3) by spray freeze-drying to obtain the microcapsules. The invention further discloses probiotic microcapsules obtained by the preparation method. The invention further discloses an application of probiotic microcapsules in the aspect of preparation of feed additives. The probiotic microcapsules disclosed by the invention can be used for improving the intestinal micro environment of a host and enhancing the disease resistance of the host; in addition, according to the preparation method disclosed by the invention, granulation is carried out by spray freeze-drying to prepare the microcapsules, thus the energy consumption can be reduced, the grain integrity can be ensured, the embedding rate can be increased, and the survival rate of probiotics can be greatly increased.

Probiotic microcapsules as well as preparation method and application thereof

The invention discloses a method for preparing three-layer embedded bifidobacterium microcapsules, and the method comprises the following steps of: squeezing bifidobacterium glue solution into soybean oil containing an emulsifier by a pressure nozzle, thoroughly stirring, emulsifying, and then adding a calcium chloride solution and standing; after layering through standing, pouring the supernatant soybean oil out and centrifuging to obtain wet capsules, and then washing the wet capsules thoroughly by using normal saline, thereby obtaining the bifidobacterium microcapsules; mixing the bifidobacterium microcapsules with a two-layer embedding solution, oscillating and stirring, centrifuging, and then collecting the two-layer embedded bifidobacterium microcapsules; adding Tween-80 to palm oil, mixing evenly and thoroughly, and sterilizing, thereby obtaining a three-layer embedding solution; mixing the two-layer embedded bifidobacterium microcapsules with the three-layer embedding solution, oscillating and stirring, centrifuging and then collecting the three-layer embedded bifidobacterium microcapsules. The bifidobacterium is protected by the three-layer embedded microcapsule technology; and as a result, the storage periods of the bifidobacterium preparation and the product containing the bifidobacterium can be prolonged.

Method for preparing three-layer embedded bifidobacterium microcapsules

The invention discloses a method for preparing bifidobacterium microcapsules containing prebiotics, and the method comprises the following steps of: atomizing a bifidobacterium glue solution into liquid drops by virtue of a pressure nozzle and then squeezing the liquid drops into soybean oil containing an emulsifier in a volume fraction of 0.2-1.0%, wherein the volume ratio of the bifidobacterium glue solution to the soybean oil is 1:(4-7); thoroughly stirring, emulsifying for 5-20 minutes, and then adding a 1-5wt% calcium chloride solution into the emulsion, and standing for more than 20 minutes; and after layering through standing, pouring the supernatant soybean oil out, centrifuging to obtain wet capsules, and then washing the wet capsules thoroughly by using normal saline, thereby obtaining the bifidobacterium microcapsules containing prebiotics. The method has the characteristics that prebiotics are added to the bifidobacterium microcapsule product; the prebiotics are capable of promoting multiplication of bifidobacteria, thereby increasing the viable count of the bifidobacteria in the microcapsules; and after the prebiotics are added, the viable count of each gram of microcapsules is obviously increased, and can be up to 10 -10 cfug .

Method for preparing bifidobacterium microcapsules containing prebiotics

This present study is based on the single factor test and the significant factors of emulsification conditions, which influence the process of microencapsulation of B. bifidum BB28 and BB01, will be screen out by Plackett-Burman experimental. Bifidobacterium BB0I and BB28 were encapsulated by using emulsion method respectively. Several factors, such as alginate concentration, cell suspension-alginate ratios, CaCl2 concentration, Tween 80 concentration, emulsification time, encapsulating time and oil-water ratios have been investigated, the results showed that sodium alginate concentration, cell suspension-alginate ratios, encapsulating time and emulsification time have a significant effect for Bifidobacterium BB01 microcapsules; CaCl2 concentration, cell suspension-alginate ratios, sodium alginate concentration and emulsification time have a significant effect for Bifidobacterium BB28 microcapsules.

Screening of Emulsification Conditions on Microcapsulation of B. bifidum BB01 and BB28 by Plackett-burman Design

The invention discloses a method for preparing milk beverage containing Bifidobacterium microcapsules The method comprises the following steps of: uniformly mixing, in parts by weightmass, 35-50 parts of fermented milk, 5-10 parts of Bifidobacterium microcapsules, 5-10 parts of concentrated juice, 5-10 parts of sucrose, 001-002 parts of taurine, 0001-0002 parts of vitamin E, 0001-0002 parts of nicotinamide, 0001-0002 parts of zinc citrate, 015-10 parts of stabilizer and 28-50 parts of water, and homogenizing under 35-45 DEG C and 10-20Mpa so as to obtain the milk beverage containing the Bifidobacterium microcapsules According to the method, the Bifidobacterium is protected through a microcapsule technology, so that the storage life of the Bifidobacterium preparation and products containing the Bifidobacterium is effectively prolonged

Method for preparing milk beverage containing Bifidobacterium microcapsules

The invention discloses a preparation method of a bifidobacterium micro capsule containing deoxidant. The preparation method comprises the following steps of: extruding bifidobacterium liquid cement by using a pressure nozzle into soybean oil containing an emulsifier with a volume fraction of 0.2-1.0%, wherein a volume ratio of the bifidobacterium liquid cement to the soybean oil is 1:(4-7); sufficiently stirring and emulsifying 5-20 minutes; adding a barium chloride solution with a mass concentration of 1-5%, and standing for more than 20 minutes; after layering through standing, pouring out the soybean oil in an upper layer; and obtaining a wet capsule by centrifuging and sufficiently washing with physiological salt water, so as to obtain the bifidobacterium micro capsule containing the deoxidant. With the adoption of the preparation method provided by the invention, the poison caused by the contact of bifidobacterium and oxygen can be overcome during a process of preparing the micro capsule can be solved by adding the deoxidant. The capsule viable count per gram after adding the deoxidant is obviously improved to 10 -10 cfug ; and the viable count is obviously higher than the bifidobacterium micro capsule without adding the deoxidant.

Preparation method of bifidobacterium micro capsule containing deoxidant

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