The invention discloses a method for detecting interaction of proteins and relates to a detection method of proteins. The detection method comprises the following steps of: respectively cloning two interacting proteins to be detected on a bacterial two-hybrid vector and co-transforming to report bacteria; starting transcriptional expression of the report genes because of the interaction of the two target proteins, and generating special enzymes; dyeing the report bacteria co-transformed with the two target proteins by using a fluorogenic substrate, hydrolyzing the fluorogenic substrate by using the report product, that is, enzyme, to release a fluorescein, and detecting the interaction of the two target proteins in a single-bacterium level by quantitatively analyzing fluorescence intensity of the single bacterium by using a flow cytometer. The quantitative detection and correlation analysis of the protein-protein interaction in the single-bacterium level are important for accurate analysis of protein functions, certainty of signal transduction paths and discovery of new drug targets.

Method for detecting interaction of proteins

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Method for detecting interaction of proteins