The invention discloses an NAC (N-acetyl-L-Cysteine) transcription factor gene GmST2 of soybean holy bean No.9 and a plant expression vector of the GmST2. The invention further discloses application of the gene GmST2 in improvement on the salt resistant/drought tolerance in arabidopsis thaliana and soybean plants. Provided by tests, the salt resistance/drought tolerance of a transgenic plant is greatly improved compared with that of a non transgenic plant and theoretical base and practice foundation for improving the salt resistance/drought tolerance of crops through genetic engineering means are provided. The NAC transcription factor gene GmST2 can be widely applied to cultivation of salt-resisting/drought tolerant plant varieties.

NAC transcription factor gene GmST2 of soybean holy bean No.9 and application of NAC transcription factor gene GmST2

The invention provides a liquorice MYB1 gene, a protein encoded by the liquorice MYB1 gene and an application of the liquorice MYB1 gene. The nucleotide sequence of a cDNA reading frame of the liquorice MYB1 gene is shown as SEQ ID NO.1 or SEQ ID NO.2; or the sequence is a sequence which is completely complementarily paired with SEQ ID NO.1 and SEQ ID NO.2; or a nucleotide sequence which is obtained by substituting, deleting or adding one or more nucleotides on the basis of the sequences shown in SEQ ID NO.1 and SEQ ID NO.2 and has the same function; or a sequence for encoding an amino acid sequence as shown in SEQ ID NO.2. The invention also discloses a preparation method of the gene. Functional research carried out through gene cloning finds that the liquorice MYB1 gene can improve the content of plant flavonoid, especially licochalcone A, can be widely applied to improving the content of flavonoid in plants and cultivating high-flavonoid-yield plant varieties, calluses, suspension cells and hairy roots, and has good application prospects; wide application prospects are realized in the fields of medicine, beauty treatment and the like.

Glycyrrhiza uralensis MYB1 gene, protein encoded by glycyrrhiza uralensis MYB1 gene and application of glycyrrhiza uralensis MYB1 gene

The invention discloses a plant salt-tolerance related protein GsERF7 and a coding gene and application thereof. The protein is a protein with the amino acid sequence shown as SEQ ID NO. 1 or a fusionprotein obtained by ligating a label at the N end and/or C end of the protein or a protection with the same function obtained by subjecting the above amino acid sequence to substitution and/or deletion and/or addition of one or a plurality of amino acid residues. Experiments prove that GsERF7 is a transcription factor protein with DNA binding and transcription functions. The protein has an interaction with wild soybean protein kinase GsSnRK1. The expression of GsERF7 and GsSnRK1 genes in the soybean hairy root can increase the tolerance of the hairy root to salt and alkali stress. The GsERF7protein has the potential to increase the salt and alkali stress resistance of plants under the control of the GsSnRK1 protein, and has important application value for agricultural molecular breeding.

Plant salt-tolerance related protein GsERF7 and coding gene and application thereof

The invention relates to a corn development regulation protein, a coding gene thereof, and an application of the protein and the coding gene. The corn development regulation protein is a protein a) composed of an amino acid residue sequence represented by sequence 2 in a sequence table, or a protein b) obtained by substituting and/or deleting and/or adding one or more amino acids to the amino acid sequence represented by the sequence 2 in the sequence table, associating with corn development regulation and derived from the protein a). The invention relates to the coding gene of the corn development regulation protein. Over-expression of the corn development regulation protein and the coding gene promotes the germination of plant seeds, reduces the plant height and promotes branch formation, and the protein and the gene have great application prospect in the plant breeding field.

Corn development regulation protein, coding gene thereof, and application of protein and coding gene

The present invention relates to a method for preparation of soybean leaves having a high content of an isoflavone derivative in a dark condition and soybean leaves having a high content of an isoflavone derivative prepared thereby. Specifically, treatment of a soybean plant 20 days to 60 days after seeding with a predetermined concentration of ethylene in a dark condition was found to accumulate higher concentrations of isoflavone derivatives in soybean leaves than treatment with ethephon in a light condition, which requires a high level of energy. Therefore, when used, the method of the present invention can economically and quickly prepare soybean leaves having a very high content of isoflavones, and the soybean leaves having a high content of isoflavone derivatives, an extract of the soybean leaves, and a fraction of the extract can be advantageously used as a food and medicine material against diseases caused by estrogen unbalance and deficient antioxidant activity.

Method for preparation of soybean leaf having high content of isoflavone derivative in dark condition and soybean leaf having high content of isoflavone derivative prepared thereby

The invention relates to a soybean ethylene responsive factor (ERF) transcription factor, and a coding gene and salt tolerance application thereof, and belongs to the field of genetic engineering The amino acid sequence of the soybean ERF transcription factor is Sequence No2, the amino acid sequence of the coding gene of the soybean ERF transcription factor is Sequence No1, and the invention provides application of the soybean ERF transcription factor to salt tolerance of a transgenic plant A GmERF7 transcription factor gene related with the soybean salt tolerance is cloned, and the expression mode of the gene in soybean, the in-vitro combination of the gene with an adversity element, and transcriptional regulation activity of the gene are researched, the salt tolerance of wild type tobacco and tobacco with trans GmERF7 gene is compared, basis is provided for the effective application of the soybean ERF transcription factor, and the invention has significance for improvement on stress resistance of plants, particularly cultivation of soybean varieties with high salt tolerance

Soybean ethylene responsive factor (ERF) transcription factor, and coding gene and salt tolerance application thereof

The invention relates to an application of a soybean MYB transcription factor gene in improvement of soybean isoflavone biosynthesis and belongs to the technical field of genetic engineering. The GmMYB9 gene is 1188bp and encodes 395 amino acids; a yeast one-hybrid experiment result indicates that GmMYB9 has transcriptional activation activity, and a subcellular localization result proves that GmMYB9 is located in a cell nucleus; a real-time fluorescence quantification PCR (polymerase chain reaction) result shows that expression tendency of GmMYB9 in a soybean development process from immature embryos to mature seeds is consistent with accumulation tendency of isoflavone, and the expression quantity of GmMYB9 in a variety with high isoflavone content is higher than that of GmMYB9 in a variety with low isoflavone content; the isoflavone content of seeds and leaves of T2-generation transgenic soybeans with the transformed GmMYB9 gene is increased, therefore, the GmMYB9 gene participates in regulation of the soybean isoflavone biosynthesis and has great significance in cultivation of the soybean varieties with high isoflavone content.

Application of soybean MYB transcription factor gene in improvement of soybean isoflavone biosynthesis

The invention relates to a transcription factor ERF related to soybean stress, a coding gene thereof, and application of the coding gene, and belongs to the field of plant gene engineering. The amino acid sequence of the transcription factor ERF related to soybean stress is shown as a Sequence No.2, and the nucleotide sequence of the coding gene of the transcription factor is shown as a Sequence No.1. A soybean GmERF6 transcription factor gene related to stress induction is cloned, the expression mode and transcription regulator activity of the gene are researched, the drought resistance of wild arabidopsis is compared with that of a GmERF6 gene-transformed arabidopsis, a basis is provided for effectively applying the GmERF6 gene, and the GmERF6 gene has important significance for improving the stress resistance of plants, particularly breeding drought-resistant soybean varieties.

Transcription factor ERF related to soybean stress, coding gene thereof and application of coding gene

The invention relates to an application of GmbHLH transcription factor in promotion of synthesizing soy isoflavone, belonging to the field of plant genetic engineering. The amino acid sequence of the GmbHLH transcription factor is shown as SEQ ID No.2, the nucleotide sequence of the encoding gene is shown as SEQ ID No.1. The selected GmbHLH transcription factor is only expressed in soy cell nucleus, and the content of the soy isoflavone can be increased by regulating the expression quantity of gene through combination with DNA sequence. No metabolites are generated during the whole process of GmbHLH as a transcription factor, so that the hidden potential hazard of the transgenic metabolism can be reduced to the greatest extent.

Application of GmbHLH transcription factor in promotion of synthesizing soy isoflavone

The invention relates to a soybean C2H2 type single zinc finger protein transcription factor and a coding gene and application thereof, and belongs to the field of plant genetic engineering The aminoacid sequence of the soybean C2H2 type single zinc finger protein transcription factor is SEQ ID N02 and is used in breeding cold-tolerant soybean varieties C2H2 type double zinc finger protein isproved to play an important role in stress, especially cold tolerance, in domestic and foreign research, while single zinc finger protein plays an irreplaceable role in plant growth and development In the invention, the single zinc finger protein gene is dug successfully, in addition to verifying the relative function of regulating growth and development, focuses on whether the gene has the function of cold tolerance or not Finally, through a series of experiments, a bifunctional gene that is involved in the regulation of growth and development and has unique cold tolerance is found, which also lays a solid foundation for the later in-depth functional exploration, and plays an important role in improving the cultivation of new multi-functional germplasm resources

Soybean C2H2 type single zinc finger protein transcription factor and coding gene and application

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