Whole-cell biocatalysts provide unique advantages and have been widely used for the efficient biosynthesis of value-added fine and bulk chemicals, as well as pharmaceutically active ingredients. What is more, advances in synthetic biology and metabolic engineering, together with the rapid development of molecular genetic tools, have brought about a renaissance of whole-cell biocatalysis. These rapid advancements mean that whole-cell biocatalysts can increasingly be rationally designed. Genes of heterologous enzymes or synthetic pathways are increasingly being introduced into microbial hosts, and depending on the complexity of the synthetic pathway or the target products, they can enable the production of value-added chemicals from cheap feedstock. Metabolic engineering and synthetic biology efforts aimed at optimizing the existing microbial cell factories concentrate on improving heterologous pathway flux, precursor supply, and cofactor balance, as well as other aspects of cellular metabolism, to enhance the efficiency of biocatalysts. In the present review, we take a critical look at recent developments in whole-cell biocatalysis, with an emphasis on strategies applied to designing and optimizing the organisms that are increasingly modified for efficient production of chemicals.

/pdf/whole-cell-biocatalysts-by-design-5g1svddhss.pdf

Whole-cell biocatalysts by design

https://escholarship.org/content/qt5mz1s1pz/qt5mz1s1pz.pdf?t=p9sqjd

Escherichia coli as a host for metabolic engineering.

Superhydrophobic anti-icing coatings with self-deicing property using melanin nanoparticles from cuttlefish juice

2-Phenylethanol (2-PE) and 2-phenethyl acetate (2-PEA) are valuable generally recognized as safe flavoring agents widely used in industry. Perfumes, pharmaceuticals, polishes, and personal care products, are some of the final products using these compounds as additives due to their rose-like odor. Also, 2-PE is used in disinfectants, pest control, and cleaning products due to its biocide capability. Although most of these additives production are derived from chemical synthesis, the current trend of consumers to prefer natural products has contributed to the development of biotechnological approaches as an alternative way to obtain natural 2-PE and 2-PEA. The most efficient route to bioproduce these compounds is through the bioconversion of L-phenylalanine via the Ehrlich pathway, and most of the advances have been focused on the development of this process. This review compiles the most recent developments in the biotechnological production of 2-PE and 2-PEA, indicating the most studied strains producing 2-PE and 2-PEA, the current advances in the in situ product recovery in liquid systems, an overview of the strain developments, and the progress in the use of residue-based systems. Future research should address the need for more sustainable and economic systems such as those using wastes as raw materials, as well as the scale-up of the proposed technologies.

Bioprocesses for 2-phenylethanol and 2-phenylethyl acetate production: current state and perspectives

Recent advances of organogels: from fabrications and functions to applications

A genetically engineered bacterium producing 2-phenylethanol and an applying method thereof are disclosed. The recombinant bacterium is constructed by coexpressing aromatic amino acid aminotransferase and exogenous phenylpyruvate decarboxylase (Aro10) (or indole-3-pyruvic acid decarboxylase), phenylethanol dehydrogenase or phenylacetaldehyde reductase in escherichia coli to obtain the recombinant bacterium producing the 2-phenylethanol. Glutamate dehydrogenase is coexpressed on this basis to achieve self-balancing cofactor circulation, thus increasing the yield of the 2-phenylethanol. The conversion ratio of the 2-phenylethanol prepared through the recombinant bacterium is high. The bacterium and the method have important application value in production of the 2-phenylethanol in the future.

A genetically engineered bacterium producing 2-phenylethanol and an applying method thereof

The invention discloses a genetically engineered bacterium capable of producing D-phenyllactic acid as well as a construction method and an application of the genetically engineered bacterium. The construction method comprises a step of transferring genes related to the synthesis of the D-phenyllactic acid into a host bacterium, so that the recombinant bacterium capable of producing the D-phenyllactic acid is obtained, wherein the genes related to the synthesis of the D-phenyllactic acid include an aromatic amino acid transferase coding gene, a D type lactic dehydrogenase coding gene and an NADH dependent glutamate dehydrogenase coding gene. The genetically engineered bacterium capable of producing the D-phenyllactic acid, which is constructed by the construction method provided by the invention, has the following advantages in the production of the D-phenyllactic acid: a cofactor circulating system is constructed, so that the supply of reduction force is enhanced; a cofactor circulating efficiency is improved by virtue of such additives as glutamic acid, [alpha]-ketoglutaric acid and the like, so that the synthesis amount of the D-phenyllactic acid is further improved; and by virtue of a whole-cell catalysis method, a D-phenyllactic acid production process is simplified, and a production cycle is shortened; therefore, the genetically engineered bacterium can play an important role in the production of the D-phenyllactic acid in the future.

Genetically engineered bacterium capable of producing N-acetylglutamate (NAG) as well as construction method and application of genetically engineered bacterium

Cofactor self-sufficient whole-cell biocatalysts for the production of 2-phenylethanol.

Ice-nucleating proteins (INPs) are the most effective ice-nucleating agents that play a significant role in preventing freeze injuries in freeze-tolerant organisms. INPs promote ice nucleation in t...

https://www.chinesechemsoc.org/doi/pdf/10.31635/ccschem.021.202000648

Bioinspired Anti-Icing Hydrogel Enabled by Ice-Nucleating Protein

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A genetically engineered bacterium producing 2-phenylethanol and an applying method thereof

Genetically engineered bacterium capable of producing N-acetylglutamate (NAG) as well as construction method and application of genetically engineered bacterium

Cofactor self-sufficient whole-cell biocatalysts for the production of 2-phenylethanol.

Bioinspired Anti-Icing Hydrogel Enabled by Ice-Nucleating Protein