The invention belongs to the technical field of magnetic bead application, and discloses a magnetic bead suspension for nucleic acid purification and screening and application thereof. The magnetic bead suspension comprises a carboxylated modified nano magnetic bead, PEG polyethylene glycol, sodium chloride and/or magnesium chloride, ethylenediaminetetraacetic acid, Tris-HCl, N-sodium lauroyl sarcosinate and Tween 20, a solvent is water, and the concentration of the nano magnetic bead is 0.1-5 mg/mL. The magnetic bead suspension can purify nucleic acid with a recovery rate of up to 96.42%, andhave a good DNA fragment screening function. The magnetic bead suspension has stable performance, is suitable for high-throughput sample operation, has low cost and high flexibility, and is suitablefor use in various workstations.

Magnetic bead suspension for nucleic acid purification and screening and application thereof

The invention provides a kit for extracting free nucleic acid and an application method thereof, belonging to the technical field of nucleic acid extraction. The kit comprises protease K, a lysis buffer solution Buffer CL, an adsorption binding solution Buffer CB, isopropanol, a first cleaning solution Buffer GW1, a second cleaning solution Buffer GW2 and an elution buffer solution Buffer EBL. Theapplication method comprises the following steps: taking a body fluid sample, centrifuging the body fluid sample, taking a supernate, putting the supernate into a clean centrifuge tube, adding the protease K and the lysis buffer solution Buffer CL into the collected supernate, adding the adsorption binding solution Buffer CB mixed with isopropanol into a lysis mixture, and transferring the mixedsolution into a silica gel adsorption column for negative-pressure suction filtration; sequentially washing the silica gel adsorption column by using the first cleaning solution Buffer GW1, the secondcleaning solution Buffer GW2 and absolute ethyl alcohol, carrying out centrifugal treatment, and conducting airing at room temperature to obtain an impurity-removed silica gel adsorption column adsorbed with cfDNA; and eluting the silica gel adsorption column by using the Buffer EBL, and centrifugally collecting eluate to obtain cfDNA. The kit provided by the invention is good in compatibility, and the extracted and purified cfDNA is high in yield, good in quality and low in cost.

Kit for extracting free nucleic acid and application method thereof

The invention relates to the technical field of molecular biology and particularly relates to a magnetic bead reagent applied to screening super-long fragment nucleic acids and a screening method. Thereagent comprises silicon oxide hydroxyl magnetic beads with the concentration of 20-800ng/uL, Tris-HCl with the concentration of 5-50mmol/L, sodium chloride with the concentration of 1-60mol/L, sodium laurate with the concentration of 0.1-20mol/L, guanidine hydrochloride with the concentration of 0.1-10mol/L, NaN3 with the concentration of 0.1-100mol/L, polyethylene glycol with the volume percent concentration of 1-25% and EDTA (Ethylene Diamine Tetraacetic Acid) with the pH value of 8 and the concentration of 0.1-10mmol/L. The reagent aims at the problem in the prior art that the large-fragment DNA recovery effect is poor in the high-throughput sequencing library construction process, large fragments are separated and screened by using magnetic beads by designing and optimizing the buffer solution of the magnetic beads, the magnetic bead reagent is particularly applicable to the long-fragment single-molecule sequencing of PacBio, Nanopore and the like, the fragment length of the sequencing data can be greatly improved, and better sequencing results can be obtained.

Magnetic bead reagent applied to screening super-long fragment nucleic acids and screening method

The invention provides a magnetic bead sorting liquid for DNA separation. 51 ml of the magnetic bead sorting liquid includes 1 ml of GE magnetic beads, 5-10 g of PEG, 12.5-50 mg of Tween 20, 50-125 mmol of NaCl, 0.25-1 mmol of Tris, 0.025-0.1 mmol of EDTA and 0.085-0.34 mmol of HCl. The invention also provides a preparation method for preparing the magnetic bead sorting liquid. The preparation method takes the GE magnetic beads as a raw material, comprises a GE magnetic bead cleaning step and a GE magnetic bead resuspending step, and has the advantages of simplicity and stability in operation,short consumed time and low cost. The prepared magnetic bead sorting liquid has the functions of DNA purification and fragmentation screening, can be kept stable for a long time, does not need further chemical modification, improves the DNA separation and purification efficiency, can replace imported magnetic beads, and is suitable for large-scale library building production.

Magnetic bead sorting liquid for DNA separation, and preparation method thereof

The invention relates to a universal type nucleic acid extraction kit and a using method thereof. The universal type nucleic acid extraction kit comprises a first reagent, a second reagent, a third reagent and a fourth reagent, wherein the first reagent is a water solution comprising 2.0-5.0 M guanidine salt, a 2.0-5.0 mM metal ion complexing agent, a 100.0-300.0 nM non-ionic surface active agentand 1.0-5.0 M isopropanol; the second reagent is a water solution comprising 20.0-40.0 mM citric acid and a 10.0-30.0 mM metal ion complexing agent, and the pH value of the second reagent is 3.5-4.5;the third reagent is a water solution comprising 2.0-10.0 mM citric acid and a 1.0-5.0 mM metal ion complexing agent, and the pH value of the third reagent is 3.5-4.5; the fourth reagent is a water solution comprising 5.0-30.0 mM trihydroxymethyl aminomethane and 1.0-15.0 mM tri(hydroxymethyl) amino methane hydrochloride. The universal type nucleic acid extraction kit and the using method thereofhave the advantages of being high in universality, high in cleavage ratio, simple in operation and high in consistency and stability.

Universal type nucleic acid extraction kit and using method thereof

The invention relates to a nucleic acid purification reagent based on a magnetic bead method and a kit. The nucleic acid purification reagent is prepared from a water solution containing the followingsubstances in percentage by mass: 1 to 5 percent of carboxyl-modified magnetic beads, 11.78 to 23.56 percent of sodium chloride, 10 to 20 percent of polyethylene glycol, 0.01 to 0.05 percent of N-sodium lauroyl sarcosine, 0.16 to 0.79 percent of Tris-HCl, 0.5 to 2 percent of EDTA and 0.5 to 2 percent of tween-20. A nucleic acid purification recovery kit comprises the nucleic acid purification reagent, a washing buffer solution and an elution buffer solution. The nucleic acid purification reagent and the kit have the advantages that the nucleic acid recovery efficiency is higher than the recovery rate of the existing magnetic bead type reagent and kit; the recovery loss on trace samples is lower; the recovery efficiency on nucleic acid fragments being about 100bp or smaller nucleic acid fragments is better.

Nucleic acid purification reagent based on magnetic bead method

A urine free DNA extraction kit comprise lysate, detergent A, detergent B, eluent and hydroxyl magnetic beads, wherein that lysate comprise guanidinium salt, metal chelating agent, surfactant, alcohol. Tris-HCl and sodium chloride, detergent A include guanidinium salts, metal chelate agents, surfactants, ethanol and tris-HCl, ethanol, 30%-40% by volume. The detergent B includes metal chelating agent, surfactant, ethanol and Tris-HCl. The volume concentration of HCl and ethanol is 75% -85%. The invention also provides a method for extracting urine free DNA by using the kit. The kit provided bythe invention has high extraction efficiency for free DNA in urine and selectively extracts nucleic acid fragments of more than 100 bp.

A urine free DNA extraction kit and an extraction method thereof

The invention relates to a kit for detecting a bladder cancer based on a high-throughput sequencing technique. The kit comprises bladder cancer mutant gene multiple PCR (polymerase chain reaction) specific primers shown from SEQ ID NO.1 to SEQ ID NO.64 and joint elements. According to the kit, a plurality of bladder cancer mutant gene loci can be simultaneously amplified through urine nucleic acid, so that a sequencing library is built, bladder cancer risks of detected people are analyzed through high-throughput sequencing and biological information, the kit is convenient in sampling, high inaccuracy and noninvasive, and the pain of a patient is relieved.

Kit for detecting bladder cancer based on high-throughput sequencing technique

The invention relates to a kit for prenatal non-invasive paternity testing. The kit comprises a part of or all the primers in a primer set for detecting 86 SNP loci. The kit of the invention can realize prenatal non-invasive paternity testing by using maximal 86 SNP loci. The kit and a detection method provided by the invention can realize high-sensitivity, high-throughput and low-cost detection,and can obtain sequences with an average depth of 20, 000 by using only 5 M of data; and with the kit and the detection method, non-invasive early-stage detection of kinship can be realized before thebirth of a child, the accuracy of the detection exceeds 99.99%, and families with very close kinship can also be perfectly distinguished.

Kit and method for prenatal non-invasive paternity testing

Handle everyday research tasks with reliable, citation-backed results

Your personal Research Agent to handle research tasks with citation-backed results

Popular Tasks used by Researchers

How can I help with your research?

Meet SciSpace

Get more enhanced response by uploading the PDFs you want me to reference.

No relevant PDFs in your library

SciSpace is the AI research assistant for academics. Run systematic literature reviews on 280M+ papers, and write papers with cited sources. Trusted by 1M+ students, PhDs & researchers.

SciSpace | AI for Research

Analyze PDFs

Code & Manuscripts

Funding & Grants

Literature & Patents

Medical & Clinical Data

Systematic Review

Visualize & Present

Web & Data

Build a Google Scholar-like website for your research.

Build a website

Create charts and images for your research

Create a Chart

Write a paper for submission to a journal

Draft a manuscript

Patent Search

Design eye-catching scientific posters in minutes.

Scientific Poster Generation

Systematic Literature Review

One task is running at the moment. Your messages will be shown right after.

Drag and drop or click here to browse

Loved by <highlight>1 million+</highlight> researchers

Extract a list of specific topics and their sources from unstructured text

Topics

Compare and analyze relevant papers that matches with your search

Papers

Get insights from PDFs and bookmarked papers from your library

My library

Recent searches

Try searching for:

Catch AI-generated content in scholarly and non-scholarly content

{ai} Detector

Ai Writer

Get PDF Summaries, highlighted text explanations 

Chat with PDF

Effortlessly create in-text citations and bibliographies in APA and 2,500 other formats

Citation generator

Get explanations, summaries, and answers on academic papers

Ease up your research workflow with {scispace}'s cohort of exciting AI tools

Elevate your academic writing skills and convey your ideas the way you want

Paraphraser

Explore our range of reading and writing tools

Your file is being prepared and should be ready in a few minutes. If it's a large file, it might take a bit longer. You can close this window, and we'll email you the file when it's done.

You have reached a maximum limit of <strong>{limit}</strong> columns in the table. Remove at least <strong>1</strong> column to add or create another one.

Wang Long

Author Tools

Chat about Author

Papers

Nucleic acid purification reagent based on magnetic bead method

A urine free DNA extraction kit and an extraction method thereof

Kit for detecting bladder cancer based on high-throughput sequencing technique

Kit and method for prenatal non-invasive paternity testing