The invention discloses a method for reducing explant pollution in tea tree tissue culture process. The treatment method comprises the following steps: sterilizing a selected explant, cutting off the discolored cut part of the explant due to sterilization, cutting the part to a 1-1.5 cm long stem section with an axillary bud, inoculating the axillary bud on a culture medium containing antibiotic, inoculating 1-2 stem sections in each bottle, and placing the stem sections in a culture room for culture after being inoculated. The method disclosed by the invention combines ampicillin with streptomycin sulfate to reduce the pollution rate of the explant in the tea tree tissue culture process so as to improve the survival rate.

Method for reducing explant pollution in tea tree tissue culture process

The invention relates to a primary culture method of stem tip tissue of strawberry. The method comprises the following steps of: (1) collecting stolon, wherein the stolon collecting time is 4-7 months, and the stolon of which the tip is between the un-spread leaf and a first spread leaf and which robustly grows is selected; (2) treating the stolon: cleaning and sterilizing the collected stolon, and carrying out low-temperature treatment under 3-8 DEG C, wherein the treatment time is 72h; (3) sterilizing and inoculating in a bechtop. The primary culture method provided by the invention has the advantages that (1) the generation of the brown stain of an explant can be effectively changed, and the survival rate of the explant can be more than 90%; (2) the explant tissue can directly form into buds by an adopted culture medium, so that the happening of heteromorphosis can be avoided, and the purity of the strain characters of the later generation can be guaranteed; (3) the diameter of the explant can be effectively controlled to be less than 0.5mm, the explant can be free from virus, and the bred offspring is completely free from virus.

Primary culture method of stem tip tissue of strawberry

The invention discloses a method for obtaining regeneration plants by use of camellia oleifera hypocotyl and belongs to the field of plant cell engineering. The method mainly comprises the step that adventive buds are induced by camellia oleifera hypocotyl. Through multiplicative subculture, strong seedling culture, rooting culture, acclimatization and transplant and the like of the adventive buds, not only is a new way provided for quick propagation of favorable camellia oleifera plants, but also a solid foundation is laid for improving the resistivity of camellia oleifera, accelerating the breeding process of camellia oleifera molecules and improving the oil quality and other properties by the gene engineering method, as well as building a camellia oleifera genetic system.

Method for obtaining regeneration plants by use of camellia oleifera hypocotyl

The invention provides a method for rapid conservation propagation of black sesame by using black sesame leaves The method comprises the following steps: a, cutting off leaves with axillary buds on black sesame plants; b, properly pruning petioles of the cut leaves with the axillary buds; c, performing water planting on the pruned leaves until the petioles root; d, performing earth cultivating on the rooted leaves According to the method disclosed by the invention, conservation propagation of the leaves with the axillary buds of the black sesame is realized, the black sesame leaves with the axillary buds are subjected to water planting until the petioles root, rooted leaf explants are planted into soil, and the axillary buds form complete plants The method has the advantages of high survival rate, no need of strict external environment, low cost, short period, simplicity, reliability, suitability for producing in factories and the like

Method for rapid conservation propagation of black sesame by using black sesame leaves

The invention provides a method for quickly conserving and reproducing cotton by utilizing cotton leaves The method comprises the steps of a, cutting leaves with axillary buds from a cotton plant; b, properly trimming the petioles of the cut leaves with the axillary buds; c, performing water culture on the trimmed leaves until the petioles are rooted; and d, performing soil culture on the rooted leaves According to the method, the conservation and reproduction are carried out by utilizing the cotton leaves with the axillary buds, the cotton leaves with the axillary buds are subjected to water culture until the petioles are rooted, rooted leaf explants are planted in soil, and the axillary buds form a complete plant; the method has the advantages of high survival rate, low external environment requirement, low cost, short period, high simplicity, high reliability and the like, thereby being suitable for industrialization

Method for quickly conserving and reproducing cotton by utilizing cotton leaves

Method for detoxifying clonal tissue culture explant of Camellia oleifera Abel

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Method for detoxifying clonal tissue culture explant of Camellia oleifera Abel