The invention discloses a medium and application thereof. The medium includes a coagulant, the coagulant adopts starch, and the starch has gel consistency of greater than 65mm, hot paste viscosity of greater than 2500cP, cool paste viscosity of 3000cP, and an amylose content of 28-30%. The medium provided by the invention employs starch with specific amylopectin content and soft gel consistency properties to replace coagulant agar in the conventional medium, can realize the support function of the coagulant to a tissue culture body, greatly reduces the cost of the medium, and is conducive to promoting the commercial application of tissue culture. The starch involved in the medium provided by the invention not only can realize the support function of the coagulant to the tissue culture body, but also can effectively provide a carbon source, promote the induction and proliferation of callus, and make seedlings better grow.

Medium and application thereof

The invention discloses a breeding method of a special rice variety for processing rice vermicilli. The breeding method comprises the following steps: selecting a rice variety I of which the amylase content is less than 15% and the gel consistency is greater than or equal to 60mm as a female parent, and hybridizing the rice variety I with a rice variety II of which the amylase content is greater than or equal to 30% and the gel consistency is less than 50mm to obtain an F1 generation of seed; with the rice variety II as a male parent, carrying out back-crossing on the F1 generation once; carrying out selfing on BC1F1; in BC1F2-BC1F3 generations of plants, selecting a rice plant of which the amylase content is 30%-32% and the gel consistency is 60-70mm; in a BC1F4 generation of strain, selecting the strain of which the setback value (SBV) is less than 30RVU, the breakdown value (BDV) is greater than 50RVU and the yield per mu is greater than or equal to 400kg; and carrying out systemic breeding in BC1F5 to BC1F6, and obtaining the special rice, of which the SBV is less than 30RVU and the BDV is greater than 50RVU, for the rice vermicilli. According to the breeding method of the special rice variety for processing the rice vermicilli disclosed by the invention, the quality problems that the rice vermicilli is hard and not smooth or soft, sticky and easy to paste are solved.

Breeding method of special rice variety for processing rice vermicilli

The invention discloses a breeding method of a herbicide-resistant rice two-line sterile line. According to the breeding method, an imidazolone-herbicide-resistant rice mutant is taken as a resistantgene donor parent, an excellent two-line sterile line is taken as a recurrent parent, continuous backcross is adopted so as culture the herbicide-resistant rice two-line sterile line which is excellent in agricultural properties, possesses sterility highly consistent to that of the recurrent parent and herbicide-resistant genes; the matching success rate of excellent hybridization rice combinations is increased, excellent and high yield herbicide-resistant two-line hybridization rice is cultured, the cultured herbicide-resistant two-line hybridization rice, routine rice varieties and the recurrent parent are subjected to matching, the hybridization later generations all possesses herbicide resistance, and weed control difficult problems in rice fields are solved effectively.

Breeding method of herbicide-resistant rice two-line sterile line

The invention discloses a starch substrate and an application thereof. The starch substrate concurrently considers dual characteristics of high amylose and high amylopectin, the content of amylose is 25-28%, the glue consistency is more than 60 millimeters, the viscosity of hot pulp and the viscosity of cold glue are 2000-2500cP, the temperature is reduced to 50 DEG C, and soft and cured starch gel can be formed. According to the starch substrate disclosed by the invention, the starch substrate having specific amylose content and soft glue consistence characteristics is used for replacing animal protein gelatin, so that plant starch empty capsules can be produced, and the starch substrate can be used as a core base material for producing leisure foods including vegetarian type jelly, soft sweets and the like.

Starch substrate and application thereof

The invention discloses a method for breeding a two-line hybrid rice sterile line special for a starch matrix. The method includes taking 'Zhejiang University capsule rice No.2' as a male parent and taking two-line sterile line rice varieties as a female parent to hybridize to obtain F1-genreation seeds; subjecting the F1-genreation seeds to selfing; selecting single rice plants with starch quality character approximate to male parent character from F2-generation plants, and backcrossing the selected single rice plants with the two-line sterile line rice varieties to obtain BC1F1-generation seeds; selecting fertile plants with agronomic character approximate to that of two-line sterile lines from BC1F1-generation plants, and backcrossing the selected fertile plants with the two-line sterile line rice varieties to obtain BC2F1-genreation seeds; repeating selfing and backcrossing, and selecting single sterile plants with the starch quality character approximate to the male parent character and the agronomic character approximate to that of the two-line sterile lines from selfing generations; conducting systemic breeding for the single sterile plants so as to obtain the two-line hybrid rice sterile line special for the starch matrix. The sterile line obtained by the method has characteristics special for the starch matrix and is similar to a mainly-cultivated sterile line in agronomic character.

Method for breeding two-line hybrid rice sterile line special for starch matrix

The invention discloses a breeding method for a special three-line hybrid rice sterile line of a starch substrate. The breeding method comprises the following steps: hybridizing 'Zhejiang University Jiao Rice No.2' as a female parent and a three-line hybrid rice maintainer line as a male parent; backcrossing F1 as a female parent and a maintainer line as a male parent; backcrossing the maintainer line with a single rice plant which has starch quality traits similar to those of the female parent and is selected from BC1-2 generations; self-crossing a single plant which has agronomic traits similar to those of the maintainer line and is selected from backcrossing generations; and selecting single plants, which have starch quality traits similar to those of the female parent and agronomic traits similar to those of the maintainer line, from BC3F2 generations, test-crossing a sterile line with special rice strains of a starch substrate in pairs, retesting the sterile line and strains capable of keeping sterility in pairs so as to obtain a special three-line hybrid rice maintainer line of a starch substrate, and hybridizing the special three-line hybrid rice maintainer line as a male parent and the sterile line as a female parent so as to obtain the special three-line hybrid rice sterile line of the starch substrate. The special three-line hybrid rice sterile line of the starch substrate disclosed by the invention has short breeding period and excellent agronomic traits.

Breeding method for special three-line hybrid rice sterile line of starch substrate

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Method for breeding two-line hybrid rice sterile line special for starch matrix

Breeding method for special three-line hybrid rice sterile line of starch substrate