Two New Diterpenoid Lactones Isolated From Andrographis Paniculata

Objective To establish a dual-wavelength HPLC method for the determination of andrographolide and dehydroandrographolide in extractum andrographis.Methods The analysis was performed on Intersil C18column(250 mm × 4.6 mm,5 μm),using methanol-water(60∶40) as mobile phase,the UV detector wavelength set at 225 nm for andrographolide and 254 nm for dehydroandrographolide.Column temperature was maintained at 35 ℃ and the flow rate was 1.0 mL·min-1.Results There was good linearity within the range of 0.104 3 ~ 1.043 0 μg(r = 1.000 0,n = 5) for andrographolide and 0.099 9 ~ 0.999 0 μg(r = 1.000 0,n = 5) for dehydroandrographolide.The average recoveries of andrographolide and dehydroandrographolide were 98.53%(RSD = 1.27%,n = 6) and 101.51%(RSD = 1.10%,n = 6).Conclusions This method is highly sensitive,rapid and accurate for determination of andrographolide and dehydroandrographolide in extractum andrographis.

Simultaneous determination of andrographolide and dehydroandrographolide in extractum andrographis by dual-wavelength HPLC

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Simultaneous determination of andrographolide and dehydroandrographolide in extractum andrographis by dual-wavelength HPLC