W. Marston Linehan
National Institutes of Health
632 Papers
4.6K Citations
W. Marston Linehan is an academic researcher from National Institutes of Health. The author has contributed to research in topics: Medicine & Renal cell carcinoma. The author has an hindex of 112, co-authored 568 publications. Previous affiliations of W. Marston Linehan include Armed Forces Institute of Pathology & Science Applications International Corporation.
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Papers
Identification of a novel transcript up-regulated in a clinically aggressive prostate carcinoma
Rodrigo F. Chuaqui,Chad R. Englert,Stephen E. Strup,Cathy D. Vocke,Zhengping Zhuang,Paul H. Duray,David G. Bostwick,W. Marston Linehan,Lance A. Liotta,Michael R. Emmert-Buck +9 more
TL;DR: A novel transcript up-regulated in an aggressive prostate carcinoma was identified using degenerate zinc finger primers in microdissected tissue samples and may be helpful in quantitative comparison of known genes and identification of novel genes in micro Dissected human tissue samples.
Evaluation of Non-Formalin Tissue Fixation for Molecular Profiling Studies
John W. Gillespie,John W. Gillespie,Carolyn J.M. Best,Verena E. Bichsel,Kristina A. Cole,Susan F. Greenhut,Stephen M. Hewitt,Mamoun Ahram,Yvonne B. Gathright,Maria J. Merino,Robert L. Strausberg,Jonathan I. Epstein,Stanley R. Hamilton,Gallya Gannot,Galina V. Baibakova,Valerie S. Calvert,Michael J. Flaig,Rodrigo F. Chuaqui,Judi Herring,John Pfeifer,Emmanuel F. Petricoin,W. Marston Linehan,Paul H. Duray,G. Steven Bova,Michael R. Emmert-Buck +24 more
TL;DR: It is found that 70% ethanol fixation and paraffin embedding is a useful method for molecular profiling studies and permit recovery of DNA, mRNA, and proteins sufficient for several downstream molecular analyses.
Co-opting a Bioorthogonal Reaction for Oncometabolite Detection
Thomas Zengeya,Julie M. Garlick,Rhushikesh A. Kulkarni,Mikayla Miley,Allison M. Roberts,Youfeng Yang,Daniel R. Crooks,Carole Sourbier,W. Marston Linehan,Jordan L. Meier +9 more
TL;DR: This work reports the development of a chemical approach to detect the oncometabolite fumarate via fluorescent pyrazoline cycloadduct formation and defines a novel intersection of bioorthogonal chemistry and metabolite reactivity that may be harnessed to enable biological profiling, imaging, and diagnostic applications.
Quantitative RT-PCR gene expression analysis of laser microdissected tissue samples
Heidi S. Erickson,Paul S. Albert,John W. Gillespie,Jaime Rodriguez-Canales,W. Marston Linehan,Peter A. Pinto,Rodrigo F. Chuaqui,Michael R. Emmert-Buck +7 more
TL;DR: The protocol described here presents the rationale, technical steps, normalization strategy and data analysis necessary to generate reliable gene expression measurements of transcripts from dissected samples.
Insights into Epigenetic Remodeling in VHL-Deficient Clear Cell Renal Cell Carcinoma.
TL;DR: How VHL loss contributes to chromatin alteration at both gene promoters and enhancers/superenhancers, in both an HIF-dependent as well as independent manner, is elucidated, and how this may provide additional targets for therapeutic intervention in advanced ccRCC.