An electron microscope study of the megacaryocyte of the rat bone marrow. I. The development of the demarcation membrane system and the platelet surface coat.

The adherence to collagen of rabbit platelets labeled in vivo with (35)SO(4) (=) has been studied both in vitro and in vivo The young platelets are labeled with (35)SO(4) (=) 2-3 days after administration of the isotope to the animals We exposed platelet-rich plasma (ethylenediamine-tetraacetate, EDTA, as anticoagulant), prepared from blood taken from rabbits 54 hr after giving the (35)SO(4) (=), to collagen in vitro There was a fall in the specific radioactivity of the nonadherent platelets which indicated a selective adhesion of young platelets to the collagen In experiments designed to have most of the (35)S label in the oldest platelets it was found that exposure of plasma containing these platelets to collagen resulted in an increase in the specific radioactivity of the nonadherent platelets Similar observations were obtained when glycine-(14)C was used as a platelet label However, when DF(32)P (di-isopropyl phosphorofluoridate-(32)P), which is thought to label platelets of all ages equally, was used, the adherence of platelets to collagen did not result in any changes in the specific activity of the nonadherent platelets In in vivo studies in which we infused a collagen suspension into rabbits 54 hr after giving (35)SO(4) (=) we found that the specific radioactivity of the platelets remaining in the circulation fell This did not occur when we infused the collagen 96 hr after giving the (35)SO(4) (=) The results from these studies indicate that young platelets adhere to collagen more readily than older platelets

/pdf/the-effect-of-platelet-age-on-platelet-adherence-to-collagen-3rhjpc05vh.pdf

The effect of platelet age on platelet adherence to collagen

Isolation and characterization of glycosaminoglycans from human leukocytes and platelets.

A study was made of the relation between the pattern and topography of thrombus formation in models of various vessel configurations coupled into extracorporeal shunts in swine and the development of atherosclerosis at corresponding sites on swine aortas. The pattern and distribution of deposits formed in the models were strikingly similar to the pattern and distribution of incipient atherosclerosis at comparable sites in the vascular tree. The earliest and only consistent component of the flow chamber deposits was the blood platelet. The platelet deposits would frequently stain with oil red O. The cholesterol level of washed human platelets was found to show a good correlation with that in the plasma. This evidence suggests that deposition of particulate matter (chiefly platelets), largely determined by the hydraulic factors, may be an important factor in the early, as well as later, stages of atherosclerosis.

Encrustation and Atherosclerosis: The Analogy Between Early in Vivo Lesions and Deposits Which Occur in Extracorporeal Circulations

The high iron diamine (HID) method has been found to impart density at the ultrastructural level selectively to sites known to contain sulphated complex carbohydrates. Thus, immature primary granules in rabbit heterophils, immature precrystalloid granules in rabbit eosinophils, all granules of rabbit basophils, mouse and rat mast cells and the nucleoids of α-granules of rabbit platelets were stained by HID. Granules of mast cells in rat cervical lymph node varied in the distribution pattern of the HID-reactive component. Mucous droplets within goblets of mouse colonic epithelial cells varied in HID reactivity. Sites known to contain sialomucin but no sulphates, such as mucous cells and apical plasmalemmae in mouse rectosigmoid colon, failed to stain with HID in contrast to their reactivity for dialysed iron at the ultrastructural level. The surface of mast cells and blood cells lacked affinity for HID, indicating that the dialysed iron binding at the surfaces can be attributed to neuraminic acid. HID proved more effective than dialysed iron in visualizing acid mucosubstance in precursor forms of the crystalloid granules in the eosinophil and in mast cell granules. Inclusion of 0.5% glycerol in the HID solution enhanced staining in mouse colon.

Ultrastructural visualization of sulphated complex carbohydrates in blood and epithelial cells with the high iron diamine procedure

Uptake of radioactive sulfate by elements of the blood and the bone marrow of rats.

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Uptake of radioactive sulfate by elements of the blood and the bone marrow of rats.