Vagner Ll
Russian Academy of Sciences
9 Papers
115 Citations
Vagner Ll is an academic researcher from Russian Academy of Sciences. The author has contributed to research in topics: Complementary DNA & cDNA library. The author has an hindex of 7, co-authored 9 publications.
Chat about Author
Papers
Simple cDNA normalization using kamchatka crab duplex-specific nuclease
Pavel A. Zhulidov,Ekaterina A. Bogdanova,Alex S. Shcheglov,Vagner Ll,George L. Khaspekov,Valery B. Kozhemyako,Mikhail V. Matz,Ella A. Meleshkevitch,Leonid L. Moroz,Sergey Lukyanov,Dmitry A. Shagin +10 more
TL;DR: A novel simple cDNA normalization method that may be effectively used for samples enriched with full-length cDNA sequences and employed to normalize cDNA from nervous tissues of the marine mollusc Aplysia californica to illustrate further the efficiency of the normalization technique.
Isolation, characterization and molecular cloning of Duplex-Specific Nuclease from the hepatopancreas of the Kamchatka crab
Veronika E. Anisimova,Denis V. Rebrikov,Dmitry A. Shagin,Valery B. Kozhemyako,Natalia I Menzorova,Dmitry B. Staroverov,Rustam Ziganshin,Vagner Ll,Valery A. Rasskazov,Sergey Lukyanov,Alex S. Shcheglov +10 more
TL;DR: A new Duplex-Specific Nuclease from Kamchatka crab hepatopancreas is described, determining its primary structure and developing a preparative method for its purification, finding that DSN had unique substrate specificity, cleaving only DNA duplexes longer than 8 base pairs, or DNA in DNA-RNA hybrids.
73
[A method for the preparation of normalized cDNA libraries enriched with full-length sequences].
P A Zhulidov,Ekaterina A. Bogdanova,Alex S. Shcheglov,Irina A. Shagina,Vagner Ll,G L Khazpekov,V V Kozhemiako,Luk'ianov Sa,Dmitry A. Shagin +8 more
TL;DR: A new method is developed based on the properties of the recently characterized duplex-specific nuclease from the hepatopancreas of the Kamchatka crab that enables the normalization of cDNA samples enriched with full-length sequences without use of laborious and ineffective stages of physical separation.
44
Normalization of full-length-enriched cDNA.
Ekaterina A. Bogdanova,Ekaterina V. Barsova,Irina A. Shagina,Scheglov A,Anisimova,Vagner Ll,S. A. Lukyanov,D. A. Shagin +7 more
TL;DR: D Duplex-specific nuclease (DSN) normalization allows the generation of normalized full-length-enriched cDNA libraries to permit a high gene discovery rate and has been evaluated in various plant and animal models.
36
DSN depletion is a simple method to remove selected transcripts from cDNA populations.
Ekaterina A. Bogdanova,Irina A. Shagina,Elena Mudrik,Igor Ivanov,Peter Amon,Vagner Ll,Sergey Lukyanov,Dmitry A. Shagin +7 more
TL;DR: A novel DSN-depletion method allows elimination of selected sequences from full-length-enriched cDNA libraries, and can be applied for subsequent EST sequencing, expression cloning, and functional screening approaches.
22