Mice were immunized intradermally with 10(7) irradiated Mycobacterium leprae organisms, and draining lymph nodes were collected after 4 weeks. Lymph node cells were restimulated in vitro with soluble M. leprae antigen and accessory cells. The resulting T-cell line was propagated in vitro in the presence of M. leprae antigen, accessory cells, and interleukin-2-containing supernatants from concanavalin A-stimulated rat spleen cells. Long-term cultured T cells were Thy-1+ L3T4- Lyt-2+ as revealed by analysis with the fluorescence-activated cell sorter. From this line, T-cell clones with the same phenotype were established. The T-cell clone A4 failed to secret interleukin-2 after stimulation with antigen and accessory cells, and its growth depended on exogeneous interleukin-2. A4 T cells produced gamma interferon in an antigen-specific, H-2-restricted, and interleukin-2-dependent way. Importantly, this T-cell clone was capable of lysing bone marrow macrophages presenting M. leprae antigen. Other T-cell clones as well as native Lyt-2+ T cells from M. leprae-immunized mice were also capable of lysing bone marrow macrophages expressing M. leprae antigens. These findings suggest that specific Lyt-2+ T cells participate in the immune response to M. leprae. It is postulated that cytolysis of M. leprae-infected macrophages or Schwann cells contributes to protection against and pathogenesis of leprosy.

Mycobacterium leprae-specific Lyt-2+ T lymphocytes with cytolytic activity.

/pdf/possible-role-of-helper-and-cytolytic-t-cells-in-t1lmclq12d.pdf

Possible role of helper and cytolytic T cells in mycobacterial infections.

Protection against facultative intracellular bacteria as well as delayed-type hypersensitivity (DTH) to bacterial antigens are mediated by specific T lymphocytes (1–4). To a great extent, studies concerned with characterizing the T lymphocyte population(s) relevant to cell-mediated antibacterial immunity have been performed in the model of experimental infection of mice with Listeria monocytogenes. These investigations revealed that T lymphocytes from infected mice, after appropriate in vitro stimulation, produce multiple lymphokine activities including migration inhibition factor (MIF), macrophage activating factor (MAF), and interferon-γ (IFN-γ) (5–8) and that the T lymphocytes responsible for these activities bear the phenotype Lyt 1+23− (7–10 and Sperling, Kaufmann, Hahn, manuscript in preparation). Furthermore, it was found that both lymphokine-production in vitro and adoptive protection against L. monocytogenes in vivo are restricted by the H-21 locus of the major histocompatibility complex (MHC) (6, 11).

Role of T lymphocyte clones in cell-mediated immunity

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Role of T lymphocyte clones in cell-mediated immunity