Travis W. Murphy
Virginia Tech
8 Papers
Travis W. Murphy is an academic researcher from Virginia Tech. The author has contributed to research in topics: Chromatin immunoprecipitation & Epigenome. The author has an hindex of 7, co-authored 8 publications.
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Papers
Recent advances in the use of microfluidic technologies for single cell analysis
TL;DR: This review of the recent microfluidic developments in single cell analysis based on microwell, microchamber, and droplet platforms covers physical, chemical, and molecular biology approaches for cellular and molecular analysis including newly emerging genome-wide analysis.
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Microfluidics for genome-wide studies involving next generation sequencing
Sai Ma,Travis W. Murphy,Chang Lu +2 more
TL;DR: Recent progress on applying microfluidics to facilitate genome-wide studies and envision rapid growth in these directions, driven by the needs for testing scarce primary cell samples from patients in the context of precision medicine.
MOWChIP-seq for low-input and multiplexed profiling of genome-wide histone modifications.
TL;DR: This protocol provides instructions for the fabrication, assembly and operation of a microfluidic device used for chromatin immunoprecipitation followed by sequencing to profile histone modifications in low-input samples to reduce labor and improve reproducibility.
Microfluidic Platform for Next-Generation Sequencing Library Preparation with Low-Input Samples.
TL;DR: A microfluidic droplet-based system for NGS library preparation, capable of reducing the number of pipetting steps significantly, reducing reagent consumption by 10x, automating much of the process, while supporting extremely low DNA input requirement (10 pg per library).
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Microfluidic Low-Input Fluidized-Bed Enabled ChIP-seq Device for Automated and Parallel Analysis of Histone Modifications
TL;DR: LIFE-ChIP-seq (low-input fluidized-bed enabled chromatin immunoprecipitation followed by sequencing), an automated and high-throughput microfluidic platform capable of running multiple sets of ChIP assays on multiple histone marks in as little as 1 h with as few as 50 cells per assay is demonstrated.