Tomohiko Umei
Keio University
19 Papers
27 Citations
Tomohiko Umei is an academic researcher from Keio University. The author has contributed to research in topics: Reprogramming & Medicine. The author has an hindex of 6, co-authored 10 publications.
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Papers
Induction of human cardiomyocyte-like cells from fibroblasts by defined factors
Rie Wada,Naoto Muraoka,Kohei Inagawa,Hiroyuki Yamakawa,Kazutaka Miyamoto,Taketaro Sadahiro,Tomohiko Umei,Ruri Kaneda,Tomoyuki Suzuki,Tomoyuki Suzuki,Kaichiro Kamiya,Shugo Tohyama,Shinsuke Yuasa,Kiyokazu Kokaji,Ryo Aeba,Ryohei Yozu,Hiroyuki Yamagishi,Toshio Kitamura,Keiichi Fukuda,Masaki Ieda +19 more
TL;DR: It is demonstrated that human fibroblasts can be directly converted to iCMs by defined factors, which may facilitate future applications in regenerative medicine.
337
Induction of Cardiomyocyte-Like Cells in Infarct Hearts by Gene Transfer of Gata4, Mef2c, and Tbx5
Kohei Inagawa,Kazutaka Miyamoto,Hiroyuki Yamakawa,Naoto Muraoka,Taketaro Sadahiro,Tomohiko Umei,Rie Wada,Yoshinori Katsumata,Ruri Kaneda,Koji Nakade,Chitose Kurihara,Yuichi Obata,Koichi Miyake,Keiichi Fukuda,Masaki Ieda +14 more
TL;DR: In vitro transduction of GMT retrovirus converted cardiac fibroblasts from the infarct region into cardiomyocyte-like cells with cardiac-specific gene expression and sarcomeric structures, and quantitative RT-PCR demonstrated that FACS-sorted 3F2A-transduced cells expressed cardiac- specific genes.
252
Single-Construct Polycistronic Doxycycline-Inducible Vectors Improve Direct Cardiac Reprogramming and Can Be Used to Identify the Critical Timing of Transgene Expression
Tomohiko Umei,Hiroyuki Yamakawa,Naoto Muraoka,Taketaro Sadahiro,Mari Isomi,Sho Haginiwa,Hidenori Kojima,Shota Kurotsu,Fumiya Tamura,Rina Osakabe,Hidenori Tani,Kaori Nara,Hiroyuki Miyoshi,Keiichi Fukuda,Masaki Ieda +14 more
TL;DR: A single-construct-based polycistronic Dox-inducible vector expressing both the rtTA and TRE-controlled transgenes is developed, which could be invaluable in the study of cardiac reprogramming.
21
Regeneration of Nonhuman Primate Hearts With Human Induced Pluripotent Stem Cell-Derived Cardiac Spheroids.
Hideki Kobayashi,Shugo Tohyama,Hajime Ichimura,Noburo Ohashi,Shuji Chino,Yusuke Soma,Hidenori Tani,Yuki Tanaka,Xiao Yang,Naoko Shiba,Shin Kadota,Kotaro Haga,Taijun Moriwaki,Yuika Morita-Umei,Tomohiko Umei,Otoya Sekine,Yoshikazu Kishino,Hideaki Kanazawa,Hiroyuki Kawagishi,Mitsuhiko Yamada,Kazumasa Narita,Takafumi Naito,Tatsuichiro Seto,Koichiro Kuwahara,Yuji Shiba,Keiichi Fukuda +25 more
TL;DR: It is demonstrated that direct injection of hiPSC-CSs restores the contractile functions of injured primate hearts with an acceptable risk of posttransplant arrhythmia and provides a strong rationale for conducting clinical trials using the equivalent CM products.
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