Tomer Rotstein
Duke University
9 Papers
25 Citations
Tomer Rotstein is an academic researcher from Duke University. The author has contributed to research in topics: Medicine & Biology. The author has an hindex of 4, co-authored 5 publications.
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Papers
Harnessing type I CRISPR–Cas systems for genome engineering in human cells
Peter Cameron,Mary M. Coons,Sanne E. Klompe,Alexandra M. Lied,Stephen C. Smith,Bastien Vidal,Paul Daniel Donohoue,Tomer Rotstein,B. Kohrs,David B. Nyer,Rachel Kennedy,Lynda M. Banh,Carolyn Williams,Mckenzi S. Toh,Matthew J. Irby,Leslie S. Edwards,Chun Han Lin,Arthur L.G. Owen,Tim Künne,John van der Oost,Stan J. J. Brouns,Stan J. J. Brouns,Euan M. Slorach,Chris R. Fuller,Scott Gradia,Steven B. Kanner,Andrew May,Samuel H. Sternberg +27 more
TL;DR: This work demonstrates that highly abundant, previously untapped type I CRISPR–Cas systems can be harnessed for genome engineering applications in eukaryotic cells.
118
Patient-derived micro-organospheres enable clinical precision oncology.
Sheng Ding,C. Hsu,Zhao-xia Wang,Naveen Natesh,R. E. Millen,Marcos Negrete,N Giroux,Grecia O. Rivera,Anders B. Dohlman,Shree Bose,Tomer Rotstein,Kassandra V. Spiller,Athena Yeung,Zhiguo Sun,Chongming Jiang,Rui Xi,Benjamin D. Wilkin,Peggy M. Randon,Ian O. Williamson,Daniel Nelson,Daniel Delubac,Sehwa Oh,Gabrielle Rupprecht,James Isaacs,Jing Jia,Chao-Hu Chen,John Paul Shen,Scott Kopetz,Shannon J. McCall,Amber Smith,Nikolce Gjorevski,Antje Walz,Scott J. Antonia,Estelle Marrer-Berger,Hans Clevers,David S. Hsu,Xiling Shen +36 more
TL;DR: In this paper , the authors used droplet emulsion microfluidics with temperature control and dead-volume minimization to rapidly generate thousands of micro-organospheres (MOSs) from low-volume patient tissues, which serve as an ideal patient-derived model for clinical precision oncology.
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Mucosal Associated Invariant T (MAIT) Cell Responses Differ by Sex in COVID-19.
Chen Yu,Sejiro Littleton,Nicholas S. Giroux,Rose Mathew,Shengli Ding,Joan Kalnitsky,Yuchen Yang,Elizabeth Petzold,Hong A. Chung,Grecia O. Rivera,Tomer Rotstein,Rui Xi,Emily R Ko,Emily R Ko,Ephraim L. Tsalik,Ephraim L. Tsalik,Ephraim L. Tsalik,Gregory D. Sempowski,Thomas N. Denny,Thomas W. Burke,Micah T. McClain,Micah T. McClain,Micah T. McClain,Christopher W. Woods,Xiling Shen,Daniel R. Saban +25 more
- 13 Apr 2021
TL;DR: In this paper, the authors carried out a sex-balanced sampling of peripheral blood mononuclear cells from confirmed COVID-19 inpatients and outpatients, uninfected close contacts, and healthy controls for 36-color flow cytometry and single cell RNA-sequencing, revealing a pronounced reduction of circulating mucosal associated invariant T (MAIT) cells in infected females.
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Chromatin remodeling in peripheral blood cells reflects COVID-19 symptom severity
Nicholas S. Giroux,Shengli Ding,Micah T. McClain,Thomas W. Burke,Elizabeth Petzold,Hong A. Chung,Grecia rivera Palomino,Ergang Wang,Rui Xi,Shree Bose,Tomer Rotstein,Bradly P. Nicholson,Tianyi Chen,Ricardo Henao,Gregory D. Sempowski,Thomas N. Denny,Emily R Ko,Geoffrey S. Ginsburg,Bryan Kraft,Ephraim L. Tsalik,Christopher W. Woods,Christopher W. Woods,Christopher W. Woods,Xiling Shen +23 more
TL;DR: It is indicated that pre-seroconversion chromatin remodeling in certain innate immune populations is associated with divergence in symptom severity, and the identified transcription factors, regulatory elements, and downstream pathways provide potential prognostic markers for COVID-19 subjects.
Mucosal Associated Invariant T (MAIT) Cell Responses Differ by Sex in COVID-19
Chen Yu,Sejiro Littleton,Nicholas S. Giroux,Rose Mathew,Shengli Ding,Joan Kalnitsky,Elizabeth Petzold,Hong Chung,Grecia rivera Palomino,Tomer Rotstein,Rui Xi,Emily R Ko,Emily R Ko,Ephraim L. Tsalik,Ephraim L. Tsalik,Ephraim L. Tsalik,Gregory D. Sempowski,Thomas N. Denny,Thomas W. Burke,Micah T. McClain,Micah T. McClain,Micah T. McClain,Christopher W. Woods,Xiling Shen,Daniel R. Saban +24 more
TL;DR: These findings uncover a female-specific protective MAIT profile, potentially shedding light on reduced COVID-19 susceptibility in females.